A total of 255 porcupine stool samples and necropsied 27 porcupines were collected and studied about the presence of helminthes in 3 different districts of Cau Ngang, Cang Long and Cau Ke in Tra Vinh province. The stool analysis method showed that 76.5% of surveyed porcupines were infected by roundworms. The roundworm infection rate increased in accordance with the age of porcupines. The 24 month old porcupine group had the highest infection rate of 90%, following closely by the group from 13-24 months and the group from 3-12 months old had the infection rate of 86.7%, and 70% respectively. The least infection rate (46.7%) belonged to young porcupines (<3 months). Enterobius vermicularis, Trichuris infundibulus, and Neoascaris mackerrasae were found in porcupines at all ages. The prevalence of Enterobius vermicularis, Trichuris infundibulus, and Neoascaris mackerrasae was 38.8%, 30.2% and 7.5%; respectively. The multiple infection rate of 3 species (based on egg morphology) per individual was 50.77%, and rate of 2 species/ individual occupied 49.23%. The necropsied method illustrated that porcupines were infected by roundworms with 96.3% and this infection rate also increased by their age. The infection rate in porcupines was 88.90%, 100%, 100%; respectively on the corresponding age groups: 3-12; >13; >24 months old. In addition, Trichuris infundibulus, Enterobius vermicularis and Neoascaris mackerrasa were also found by this method. However, there was significant difference from the infection rates among species as compared with the stool analysis method. In fact, 96.3% of surveyed porcupines were infected by 2 species of Trichuris infundibulus, Enterobius vermicularis, and Neoascaris mackerrasa could be only found in 18.5% of studied porcupines.