New SPE-LC-MS/MS method for simultaneous determination of multi-class cyanobacterial and algal toxins

Zervou, Sevasti‐Kiriaki; Christophoridis, Christophoros; Kaloudis, Triantafyllos; Triantis, Theodoros M.; Hiskia, Anastasia

doi:10.1016/j.jhazmat.2016.07.020

Cited by 115 publications

(91 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, LC-MS can be used simultaneously for several classes of compounds. Zervou et al (2017) provide a good example of this multitoxin approach where 12MCs, NOD, CYN, ANTX-a, and the marine algal toxins domoic acid and okadaic acid were concentrated by a dual-cartridge solid-phase extraction procedure and determined in a single LC-MS/MS run. One obvious complication with LC-MS/MS analyses is the need for reference materials which are currently not commercially available for all cyanotoxins.…”

Section: Occurrence Of Cyanobacteria and Cyanotoxins And Methods Formentioning

confidence: 99%

Toxic cyanobacteria and cyanotoxins in European waters – recent progress achieved through the CYANOCOST Action and challenges for further research

et al. 2017

Self Cite

View full text Add to dashboard Cite

This review aims to summarise the outcomes of some recent European research concerning toxic cyanobacteria and cyanotoxins, with an emphasis on developments within the framework of the CYANOCOST Action: COST Action ES1105, Cyanobacterial Blooms and Toxins in Water Resources: Occurrence, Impacts and Management. Highlights of the Action include phycological and ecological studies, development of advanced techniques for cyanotoxin analysis, elucidation of cyanotoxin modes of action, management techniques to reduce cyanobacterial mass development, and research on methods and practices for cyanotoxin removal during drinking water treatment. The authors have identified a number of gaps in knowledge. Proposed directions for future research on toxic cyanobacteria and cyanotoxins are also discussed.

show abstract

Section: Occurrence Of Cyanobacteria and Cyanotoxins And Methods Formentioning

confidence: 99%

Toxic cyanobacteria and cyanotoxins in European waters – recent progress achieved through the CYANOCOST Action and challenges for further research

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has been described some cyanobacterial strains are able to produce different cyanotoxins or different congeners of a cyanotoxin . There are several studies concerning the co‐occurrence of MCs‐ and CYN‐producing cyanobacteria as well as the presence of combinations of both cyanotoxins at the same time . In this sense, Graham et al found that MCs are the most abundant cyanotoxins in blooms from USA, being present in all of them.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic and morphological effects of microcystin‐LR, cylindrospermopsin, and their combinations on the human hepatic cell line HepG2

Gutiérrez-Praena

Guzmán-Guillén

Pichardo

et al. 2018

Environmental Toxicology

View full text Add to dashboard Cite

Cylindrospermopsin (CYN) and Microcystin-LR (MC-LR) are toxins produced by different cyanobacterial species, which are found mainly in freshwater reservoirs. Both of them can induce, separately, toxic effects in humans and wildlife. However, little is known about the toxic effects of the combined exposure, which could likely happen, taking into account the concomitant occurrence of the producers. As both cyanotoxins are well known to induce hepatic damage, the human hepatocellular HepG2 cell line was selected for the present study. Thus, the cytotoxicity of both pure cyanotoxins alone (0-5 μg/mL CYN and 0-120 μg/mL MC-LR) and in combination for 24 and 48 h was assayed, as long as the cytotoxicity of extracts from CYN-producing and nonproducing cyanobacterial species. The potential interaction of the combination was evaluated by the isobologram or Chou-Talalay's method, which provides a combination index as a quantitative measure of the two cyanotoxins interaction's degree. Moreover, a morphological study of the individual pure toxins and their combinations was also performed. Results showed that CYN was the most toxic pure cyanotoxin, being the mean effective concentrations obtained ≈4 and 90 μg/mL for CYN and MC-LR, respectively after 24 h. However, the simultaneous exposure showed an antagonistic effect. Morphologically, autophagy, at low concentrations, and apoptosis, at high concentrations were observed, with affectation of the rough endoplasmic reticulum and mitochondria. These effects were more pronounced with the combination. Therefore, it is important to assess the toxicological profile of cyanotoxins combinations in order to perform more realistic risk evaluations.

show abstract

“…The determination of CYN, ATX‐a, NOD, and 12 MCs ([D‐ Asp3]MC‐RR, MC‐RR, MC‐YR, MC‐HtyR, [D‐ Asp3]MC‐LR, MC‐LR, MC‐HilR, MC‐WR, MC‐LA, MC‐LY, MC‐LW, MC‐LF) was conducted according to the LC‐MS/MS method described by Zervou et al. ().…”

Section: Methodsmentioning

confidence: 99%

Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea

Konstantinou

Voultsiadou

Panteris

et al. 2019

Journal of Phycology

Self Cite

View full text Add to dashboard Cite

Cyanobacterial diversity associated with sponges remains underestimated, though it is of great scientific interest in order to understand the ecology and evolutionary history of the symbiotic relationships between the two groups. Of the filamentous cyanobacteria, the genus Leptolyngbya is the most frequently found in association with sponges as well as the largest and obviously polyphyletic group. In this study, five Leptolyngbya‐like sponge‐associated isolates were investigated using a combination of molecular, chemical, and morphological approach and revealed a novel marine genus herein designated Leptothoe gen. nov. In addition, three new species of Leptothoe, Le. sithoniana, Le. kymatousa, and Le. spongobia, are described based on a suite of distinct characters compared to other marine Leptolyngbyaceae species/strains. The three new species, hosted by four sponge species, showed different degrees of host specificity. Leptothoe sithoniana and Le. kymatousa hosted by the sponges Petrosia ficiformis and Chondrilla nucula, respectively, seem to be more specialized than Le. spongobia, which was hosted by the sponges Dysidea avara and Acanthella acuta. All three species contained nitrogen‐fixing genes and may contribute to the nitrogen budget of sponges. Leptothoe spongobia TAU‐MAC 1115 isolated from Acanthella acuta was shown to produce microcystin‐RR indicating that microcystin production among marine cyanobacteria could be more widespread than previously determined.

show abstract

New SPE-LC-MS/MS method for simultaneous determination of multi-class cyanobacterial and algal toxins

Cited by 115 publications

References 54 publications

Toxic cyanobacteria and cyanotoxins in European waters – recent progress achieved through the CYANOCOST Action and challenges for further research

Toxic cyanobacteria and cyanotoxins in European waters – recent progress achieved through the CYANOCOST Action and challenges for further research

Cytotoxic and morphological effects of microcystin‐LR, cylindrospermopsin, and their combinations on the human hepatic cell line HepG2

Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea

Contact Info

Product

Resources

About