New sources of rice blast resistance obtained from Thai indigenous upland rice germplasm

Chumpol, Atitaya; Chankaew, Sompong; Saepaisan, Suwita; Monkham, Tidarat; Sanitchon, Jirawat

doi:10.1007/s10681-018-2267-3

Cited by 5 publications

(1 citation statement)

References 20 publications

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“…Disease symptoms occur in all stages of plant growth, beginning with blast discoloration and wilting of the foliage [4]. Neck blast can be found at the flowering stage, accelerating plant death [5]. Severe damage was also observed within areas of intensive planting with high doses of nitrogen application [6].…”

Section: Introductionmentioning

confidence: 99%

Improvement of a RD6 Rice Variety for Blast Resistance and Salt Tolerance through Marker-Assisted Backcrossing

et al. 2020

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RD6 is one of the most favorable glutinous rice varieties consumed throughout the north and northeast of Thailand because of its aroma and softness. However, blast disease and salt stress cause decreases in both yield quantity and quality during cultivation. Here, gene pyramiding via marker-assisted backcrossing (MAB) using combined blast resistance QTLs (qBl 1, 2, 11, and 12) and Saltol QTL was employed in solving the problem. To pursue our goal, the RD6 introgression line (RGD07005-12-165-1), containing four blast-resistant QTLs, were crossed with the Pokkali salt tolerant variety. Blast resistance evaluation was thoroughly carried out in the fields, from BC2F2:3 to BC4F4, using the upland short-row and natural field infection methods. Additionally, salt tolerance was validated in both greenhouse and field conditions. We found that the RD6 “BC4F4 132-12-61” resulting from our breeding programme successfully resisted blast disease and tolerated salt stress, while it maintained the desirable agronomic traits of the original RD6 variety. This finding may provide a new improved rice variety to overcome blast disease and salt stress in Northeast Thailand.

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Section: Introductionmentioning

confidence: 99%

Improvement of a RD6 Rice Variety for Blast Resistance and Salt Tolerance through Marker-Assisted Backcrossing

et al. 2020

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Identification of Rhizoctonia solani, as the cause of rice sheath blight and the source of its resistance, from Thai indigenous lowland rice germplasm

et al. 2021

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Integrated genetic analysis of leaf blast resistance in upland rice: QTL mapping, bulked segregant analysis and transcriptome sequencing

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Chen

et al. 2022

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Background Elite upland rice cultivars have the advantages of less water requirement along with high yield but are usually susceptible to various diseases. Rice blast caused by Magnaporthe oryzae is the most devastating disease in rice. Identification of new sources of resistance and the introgression of major resistance genes into elite cultivars are required for sustainable rice production. Results In this study, an upland rice genotype UR0803 was considered an emerging source of blast resistance. An F2 mapping population was developed from a cross between UR0803 and a local susceptible cultivar LTH. The individuals from the F2 population were evaluated for leaf blast resistance in three trials at four-time points (0, 24, 48, and 72 hours) after inoculation. Bulked segregant analysis by high-throughput sequencing and SNP-index algorithm was performed to map the candidate region related to disease resistance trait. A major quantitative trait locus (QTL) for leaf blast resistance was identified on chromosome 11 in an interval of 1.61 Mb genomic region. The candidate region was further shortened to a 108.9 Kb genomic region by genotyping the 955 individuals with fourteen SNP markers. Transcriptome analysis was further performed between the resistant and susceptible parents, yielding a total of 5,044 differentially expressed genes (DEGs). There were four DEGs in the candidate QTL region, of which, two (Os11g0700900 and Os11g0704000) were up-regulated and the remaining (Os11g0702400 and Os11g0703600) were down-regulated in the susceptible parent after inoculation. These novel candidate genes were functionally annotated to catalytic response against disease stimulus in cellular membranes. The results were further validated by a quantitative real-time PCR analysis. Conclusion The fine mapping of a novel QTL for blast resistance by integrative BSA mapping and transcriptome sequencing enhanced the genetic understanding of the mechanism of blast resistance in upland rice. The most suitable genotypes with resistance alleles would be useful genetic resources in rice blast resistance breeding.

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New sources of rice blast resistance obtained from Thai indigenous upland rice germplasm

Cited by 5 publications

References 20 publications

Improvement of a RD6 Rice Variety for Blast Resistance and Salt Tolerance through Marker-Assisted Backcrossing

Improvement of a RD6 Rice Variety for Blast Resistance and Salt Tolerance through Marker-Assisted Backcrossing

Identification of Rhizoctonia solani, as the cause of rice sheath blight and the source of its resistance, from Thai indigenous lowland rice germplasm

Integrated genetic analysis of leaf blast resistance in upland rice: QTL mapping, bulked segregant analysis and transcriptome sequencing

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