New Sources of Anthocyanins

Máriássyová, M.; Šilhár, S.; Kováč, Milan

doi:10.1533/9781845698140.6.311

Cited by 4 publications

(8 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, the [Co(phen) 3 ] 3+ marker was accumulated from its 5×10 -7 M solution in 10 mM phosphate buffer under stirring for 120 s at an open circuit. The DP voltammogram was recorded immediately (without a medium exchange) from +0.4 to -0.5 V at the pulse amplitude of 100 mV and the scan rate of 25 mV/s.…”

Section: Methodsmentioning

confidence: 99%

“…Then, the DNA/SPE was regenerated by a removal of the accumulated [Co(phen) 3 ] 3+ ions from the DNA layer treating the sensor in solution of a high ionic strength (100 mM phosphate buffer pH 7.0) under stirring during 120 s. A negligible marker peak current was checked by the DPV record in blank. DNA damage and effect of antioxidants were detected by the measurement of the DPV marker signal (as described above) after the pretreatment of the sensor in blank solution (peak current I 0 was obtained in triplicate) or in the cleavage mixture (…”

Section: Methodsmentioning

confidence: 99%

“…The DNA redox marker [Co(phen) 3 ] 3+ can be accumulated effectively from its solution within the DNA layer on the electrode surface at both an open circuit [19-21, 24, 25] as well as a polarization of the electrode by a positive potential which is often used to stimulate association interactions of DNA [18,26]. Voltammetric behavior of [Co(phen) 3 ] 3+ at the DNA-modified gold electrode [24], glassy carbon electrode [20], carbon paste electrode [25,26] as well as carbon-based screen-printed electrode [18,19] was reported previously.…”

Section: Detection Of Dna Damagementioning

confidence: 99%

“…They are used in the food industry to delay the oxidation process [2,3]. The most active dietary antioxidants belong to the family of phenolic and polyphenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

“…This scheme is based on the known quantification of the original double stranded (ds) DNA using the [Co(phen) 3 ] 3+ complex ion as a dsDNA electrochemical label. The amount of [Co(phen) 3 ] 3+ bound to the DNA layer decreases with a decrease in the portion of original dsDNA in the course of cleavage reaction [20,21]. Two standards of phenolic acids as well as four aqueous plant extracts were tested as the antioxidants.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode

Labuda

Bučková

Heilerová

et al. 2002

Sensors

View full text Add to dashboard Cite

Abstract:A simple procedure for the voltammetric detection of antioxidative activity of plant extracts based on the protection from DNA damage at the electrode surface is reported. A disposable electrochemical DNA biosensor fabricated as a carbon-based screen-printed electrode modified by a surface layer of the calf thymus double stranded (ds) DNA was used as a working electrode in combination with a silver/silver chloride reference electrode and a separate platinum auxiliary electrode. The [Co(phen) 3 ] 3+ ion served as the dsDNA redox marker and the [Fe(EDTA)] -complex with hydrogen peroxide under the electrochemical reduction of the iron atom were used as the DNA cleavage mixture. A remarkable antioxidative activity of phenolic antioxidants such as rosmarinic and caffeic acids as standards and the extracts of lemon balm, oregano, thyme and agrimony was found which is quite in agreement with an antiradical activity determined spectrophotometrically using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Detection Of Dna Damagementioning

confidence: 99%

“…They are used in the food industry to delay the oxidation process [2,3]. The most active dietary antioxidants belong to the family of phenolic and polyphenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode

Labuda

Bučková

Heilerová

et al. 2002

Sensors

View full text Add to dashboard Cite

show abstract

Transformation of vegetable waste into value added products:

Laufenberg

Kunz

Nystroem

2003

Bioresource Technology

552

114

View full text Add to dashboard Cite

The study of irrigation influence on nutritional value of Lonicera kamtschatica - cultivar Gerda 25 and Lonicera edulis berries under the Nitra conditions during 2001-2003

Juríková¹,

Matuškovič²

2007

Hort. Sci. (Prague)

View full text Add to dashboard Cite

Two species of edible honeysuckle were studied in the trial established in the autumn 1994. The spacing used was 2.0 × 1.5 m and the experiment included irrigated as well as non-irrigated variants. The aim of this study was to provide a summary of nutritional values of Lonicera kamtschatica and Lonicera edulis berries under the Nitra conditions during the period [2001][2002][2003]. The berries were examined for the content of dry mater, sugar, organic acids, ascorbic acid, mineral substances -K, Ca, P, Mg, Na and anthocyanins from pomace and juice. The results of our experiments refer to the exceptionally high content of ascorbic acid ranging from 28.56 up to 86.96 mg/100 g, potassium 10,175 to 14,764 mg/kg and anthocyanins from pomace 6.245-17.36 g/kg. The values of ascorbic acid varied and they were statistically significantly depending on the year; similarly, only year can be taken into account as a statistically significant factor for forming content of sugar, magnesium and sodium. The content of dry mater, organic acids, potassium, calcium and anthocyanins in juice was influenced not only by year but also by species. The interaction species -year as a source of statistically significant differences is evident in the case of phosphorus and organic acids. A significant influence of irrigation on all evaluated nutrients was not confirmed. covered with black polyethylene mulch. In order to examine the influence of drip -irrigation on the nutritional value of fruit the experiment comprised 2 variants, irrigated and non-irrigated, placed in 2 blocks.The soil and climatic conditions of the site were as follows: open level, 130 m above sea level, corn processing area, clay-loam -drift, pH 6.4, mould content 3.5%, precrop black fallow, average rainfall 564 mm per year, average temperature during vegetation period 16.3°C. The actual values of average rainfall and temperature in March-June 2001-2003 are given in Tables 1 and 2. 12 shrubs were planted for each variant. The research of the chemical composition of Lonicera berries was carried out at the Plant Research Institute in Malanta in [2001][2002][2003]. The samples of edible honeysuckle were analyzed for the content of dry mater, sugar, organic acids, ascorbic acid and mineral matters -potassium (K), calcium (Ca), phosphorus (P), sodium (Na), magnesium (Mg) and for the content of anthocyanins from pomace and juice.In order to determine the content of dry matter, berries were dehumidified until constant weight was reached; organic acids were determined by the NaOH titration according to Príbela (1967) and were calculated on citric acid; the percentage of sugar content was determined by the method of SOMOGYI; the amount of ascorbic acid was obtained by polarography; mineral matters were identified in ash and the content of mineral substances was determined by atomic spectroscopy (AAS) after drying and burning berries in the drying kiln; the content of anthocyanins was determined by the method of Füleki and Francis (1968). Statistical analyses were conduct...

show abstract

New Sources of Anthocyanins

Cited by 4 publications

References 2 publications

Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode

Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode

Transformation of vegetable waste into value added products:

The study of irrigation influence on nutritional value of Lonicera kamtschatica - cultivar Gerda 25 and Lonicera edulis berries under the Nitra conditions during 2001-2003

Contact Info

Product

Resources

About