New SNP markers of the honeybee vitellogenin gene (Vg) used for identification of subspecies Apis mellifera mellifera L.

Il’yasov, R. S.; Poskryakov, A. V.; Николенко, А. Г.

doi:10.1134/s1022795415020088

Cited by 16 publications

(17 citation statements)

References 12 publications

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“…While SNP analyses can differentiate other honey bee subspecies belonging to different evolutionary groups 29 , 57 , 58 , mitogenome sequences are not able to differentiate A . m .…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial genome diversity and population structure of two western honey bee subspecies in the Republic of South Africa

Eimanifar

Kimball

Braun

2018

Sci Rep

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Apis mellifera capensis Eschscholtz and A.m. scutellata Lepeletier are subspecies of western honey bees that are indigenous to the Republic of South Africa (RSA). Both subspecies have invasive potential and are organisms of concern for areas outside their native range, though they are important bees to beekeepers, agriculture, and the environment where they are native. The aim of the present study was to examine genetic differentiation among these subspecies and estimate their phylogenetic relationships using complete mitochondrial genomes sequences. We used 25 individuals that were either assigned to one of the subspecies or designated hybrids using morphometric analyses. Phylogenetic analyses of mitogenome sequences by maximum likelihood (ML) and Bayesian inference identified a monophyletic RSA clade, subdivided into two clades. A haplotype network was consistent with the phylogenetic trees. However, members of both subspecies occurred in both clades, indicating that A.m. capensis and A.m. scutellata are neither reciprocally monophyletic nor do they exhibit paraphyly with one subspecies nested within the other subspecies. Furthermore, no mitogenomic features were diagnostic to either subspecies. All bees analyzed from the RSA expressed a substantial level of haplotype diversity (most samples had unique haplotypes) but limited nucleotide diversity. The number of variable codons across protein-coding genes (PCGs) differed among loci, with CO3 exhibiting the most variation and ATP6 the least.

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“…While SNP analyses can differentiate other honey bee subspecies belonging to different evolutionary groups 29 , 57 , 58 , mitogenome sequences are not able to differentiate A . m .…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial genome diversity and population structure of two western honey bee subspecies in the Republic of South Africa

Eimanifar

Kimball

Braun

2018

Sci Rep

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“…Genetic structure among populations was additionally investigated using STRUCTURE 2.3.3 [ 57 ]. A total of 10 6 simulations using 10 5 burn-in steps and MCMC (Markov Chain Monte Carlo algorithm) steps were run for all samples ( N = 4,388) simultaneously, considering a K interval [ 1 – 30 ] with ten iterations each. The optimal number of clusters was estimated using the ΔK method of Evanno [ 58 ].…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity and differentiation among insular honey bee populations in the southwest Indian Ocean likely reflect old geographical isolation and modern introductions

et al. 2017

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With globalization the Western honey bee has become a nearly cosmopolitan species, but it was originally restricted to the Old World. This renowned model of biodiversity has diverged into five evolutionary lineages and several geographic “subspecies.” If Apis mellifera unicolor is indubitably an African subspecies endemic to Madagascar, its relationship with honey bees from three archipelagos in the southwest Indian Ocean (SWIO) hotspot of biodiversity is misunderstood. We compared recent mtDNA diversity data to an original characterization of the nuclear diversity from honey bees in the Mascarenes and Comoros archipelagos, using 14 microsatellites, but also additional mtDNA tRNALeu-cox2 analysis. Our sampling offers the most comprehensive dataset for the SWIO populations with a total of 3,270 colonies from 10 islands compared with 855 samples from Madagascar, 113 from Africa, and 138 from Europe. Comprehensive mitochondrial screening confirmed that honey bees from La Réunion, Mauritius, and Comoros archipelagos are mainly of African origin (88.1% out of 2,746 colonies) and that coexistence with European lineages occurs only in the Mascarenes. PCA, Bayesian, and genetic differentiation analysis showed that African colonies are not significantly distinct on each island, but have diversified among islands and archipelagos. FST levels progressively decreased in significance from European and African continental populations, to SWIO insular and continental populations, and finally among islands from the same archipelago. Among African populations, Madagascar shared a nuclear background with and was most closely related to SWIO island populations (except Rodrigues). Only Mauritius Island presented clear cytoplasmic disequilibrium and genetic structure characteristic of an admixed population undergoing hybridization, in this case, between A. m. unicolor and A. m. ligustica, A. m. carnica and A. m. mellifera-like individuals. Finally, global genetic clustering analysis helped to better depict the colonization and introduction pattern of honey bee populations in these archipelagos.

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“…In the last few years, studies on Single Nucleotide Polymorphisms (SNPs) and the molecular evolution of Vg have broadened our knowledge of the functions of Vg in behaviour, immunity, longevity and social organization in the honeybee Apis mellifera (Havukainen et al, 2011;Kent et al, 2011;Ilyasov et al, 2015aIlyasov et al, , 2015b. After sequencing most of the Vg coding region in dozens of honeybee workers, Kent et al (2011) found that Vg exhibits high levels of nucleotide diversity in Ap.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, Ilyasov et al . (, ) found 26 SNPs in Vg that can be used as markers for genetic cataloguing of honeybee colonies. However, much remains to be discovered about how SNPs are involved in modulating Vg transcription in insects, although there are likely to be many such SNPs.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation of the vitellogenin gene through a fecundity‐related single nucleotide polymorphism within a GATA‐1 binding motif in the brown planthopper, Nilaparvata lugens

Sun

Kang

Cai

et al. 2018

Insect Molecular Biology

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Identifying the Single Nucleotide Polymorphisms (SNPs) with functions in insect fecundity promises to provide novel insight into genetic mechanisms of adaptation and to aid in effective control of insect populations. We previously identified several SNPs within the vitellogenin (Vg) promoter region between a high-fecundity population (HFP) and a low-fecundity population (LFP) of the brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae). Here, we found that an A-to-T (HFP allele to LFP allele) transversion at nucleotide -953 upstream of Vg in a Nilaparvata lugens GATA-1 (NlGATA-1) binding motif is associated with the level of Vg transcription. We also characterized NlGATA-1, containing a double CX CX CX C zinc finger, which has been implicated in the activation of Vg gene expression. Knockdown of the NlGATA-1 gene results in a reduced basal level of expression of the Vg gene and fewer offspring of N. lugens in vivo, whereas overexpression of NlGATA-1 in cells increased Vg promoter activity. Moreover, upon cotransfection with NlGATA-1 expression vector, the luciferase activities of Vg reporter vectors with the A allele were significantly higher than those with the T allele. These findings support a mechanism in which a SNP within the promoter of Vg is associated with the level of Vg transcription by altering the binding activity of NlGATA-1 and subsequently affecting fecundity in N. lugens.

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New SNP markers of the honeybee vitellogenin gene (Vg) used for identification of subspecies Apis mellifera mellifera L.

Cited by 16 publications

References 12 publications

Mitochondrial genome diversity and population structure of two western honey bee subspecies in the Republic of South Africa

Mitochondrial genome diversity and population structure of two western honey bee subspecies in the Republic of South Africa

Genetic diversity and differentiation among insular honey bee populations in the southwest Indian Ocean likely reflect old geographical isolation and modern introductions

Transcriptional regulation of the vitellogenin gene through a fecundity‐related single nucleotide polymorphism within a GATA‐1 binding motif in the brown planthopper, Nilaparvata lugens

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