New Skin for Old

Phillips, Tania J.

doi:10.1001/archderm.134.3.344

Cited by 93 publications

(18 citation statements)

References 52 publications

(55 reference statements)

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“…After 7 days in 3D-gel culture, there are no detectable viable EGFP-transduced cells (g), whereas those transduced with Bcl-2 are still organized into cords (h) (ϫ400). available for clinical usage (26). This matrix supported the organization of HUVEC into primitive capillary-like structures by 18 to 24 h in culture and led to the development of human EC-lined microvessels perfused by the mouse circulation after implantation.…”

Section: Discussionmentioning

confidence: 94%

In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse

Schechner

Nath

Zheng

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

309

260

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We have identified conditions for forming cultured human umbilical vein endothelial cells (HUVEC) into tubes within a threedimensional gel that on implantation into immunoincompetent mice undergo remodeling into complex microvessels lined by human endothelium. HUVEC suspended in mixed collagen͞ fibronectin gels organize into cords with early lumena by 24 h and then apoptose. Twenty-hour constructs, s.c. implanted in immunodeficient mice, display HUVEC-lined thin-walled microvessels within the gel 31 days after implantation. Retroviral-mediated overexpression of a caspase-resistant Bcl-2 protein delays HUVEC apoptosis in vitro for over 7 days. Bcl-2-transduced HUVEC produce an increased density of HUVEC-lined perfused microvessels in vivo compared with untransduced or control-transduced HUVEC. Remarkably, Bcl-2-but not control-transduced HUVEC recruit an ingrowth of perivascular smooth-muscle ␣-actin-expressing mouse cells at 31 days, which organize by 60 days into HUVEC-lined multilayered structures resembling true microvessels. This system provides an in vivo model for dissecting mechanisms of microvascular remodeling by using genetically modified endothelium. Incorporation of such human endothelial-lined microvessels into engineered synthetic skin may improve graft viability, especially in recipients with impaired angiogenesis.

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Section: Discussionmentioning

confidence: 94%

In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse

Schechner

Nath

Zheng

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

309

260

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“…For dermatologists, the most important uses are: (1) injectable collagens which are widely in use in esthetic dermatology and dermatologic surgery and (2) collagen-based implantable devices used for hemostasis in large surface wounds and for tissue substitution and/or for healing of acute and chronic wounds [6, 7, 8, 9, 10]. If one can effectively treat chronic ulcers on an outpatient basis, one can prevent expensive and lengthy hospitalization.…”

Section: Clinical Use Of Collagen Materialsmentioning

confidence: 99%

Collagen Uses in Dermatology – An Update

Ruszczak

Schwartz

1999

Dermatology

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“…1 Sulfonate-containing hydrogels are of particular interest because of their tunable swelling 2 and mechanical properties, as well as their ability to bind cationic drugs. 3,4 Chondroitin sulfate and dermatin sulfate are important natural sulfonate-containing polysaccharides that have been used in cartilage tissue engineering, 5 skin substitution, 6 and treatment of joint diseases. 7 Chondroitin sulfate has been incorporated into hydrogels to tailor their swelling, 3 elasticity, 3,8 and drug release rate, 3,9,10 and to promote adhesion of cells to hydrogels.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Injectable Sulfonate-Containing Hydrogels

et al. 2016

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Sulfonate-containing hydrogels are of particular interest because of their tunable mechanical and swelling properties, as well as their biological effects. Polysulfonate copolymers were synthesized by reacting 2-acrylamido-2-methylpropanesulfonic acid (AMPS), acrylamide (AM), and acrylic acid (AA). We found that the incorporation rate of sulfonate-containing monomer and the molecular weight of the copolymer were significantly enhanced by increasing the ionic strength of the solution. We introduced thiol groups by modifying the pendant carboxylates or copolymerizing along with a disulfide-containing monomer. The thiol-containing copolymers were reacted with a 4-arm acrylamide-terminated poly(ethylene glycol) via a thiol–ene click reaction, which was mediated by a photoinitiator, a redox initiator, or a base-catalyzed Michael-Addition. We were able to tailor the storage modulus (33–1800 Pa) and swelling capacity (1–91 wt %) of the hydrogel by varying the concentration of the copolymers. We determined that the injectable sulfonate-containing hydrogels were biocompatible up to 20 mg/mL, as observed by an electric cell–substrate impedance sensing (ECIS) technique, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using three different cell lines: human retinal pigment epithelial cells (ARPE-19), fibroblasts (NIH 3T3), and Chinese hamster ovary cells (CHO).

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New Skin for Old

Cited by 93 publications

References 52 publications

In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse

In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse

Collagen Uses in Dermatology – An Update

Synthesis and Characterization of Injectable Sulfonate-Containing Hydrogels

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