New Sexing Strains for Mediterranean Fruit Fly Ceratitis capitata: Transforming Females into Males

Saccone, Giuseppe; Pane, Andrea; Simone, A. De; Salvemini, Marco; Milano, Andreina; Annunziata, Luigi; Mauro, U.; Polito, Lino C.

doi:10.1007/978-1-4020-6059-5_7

Cited by 26 publications

(30 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, such splicing modules can be genetically modified and used to engineer strains carrying dominant female-specific lethal factors [Fu et al, 2007] or female-male-reverting factors which target disruption of the tra gene [Saccone et al, 2007]. The prospects are that the very same strategy can be applied to a wide range of pest insects, e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the notion that sex determination pathways in insects converge and are built on conserved regulatory modules offers a useful concept for designing new genetic strategies in pest control management [Saccone et al, 2007;Morrison et al, 2010]. For instance, such splicing modules can be genetically modified and used to engineer strains carrying dominant female-specific lethal factors [Fu et al, 2007] or female-male-reverting factors which target disruption of the tra gene [Saccone et al, 2007].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sex Determination in Insects: Variations on a Common Theme

Bopp

Saccone

Beye

2013

Sex Dev

Self Cite

132

144

View full text Add to dashboard Cite

Recent studies in a representative selection of holometabolous insects suggest that, despite diversity at the instructive level, the signal-relaying part of the sex-determining pathway is remarkably well conserved. In principle, it is composed of the transformer gene (tra), which acts as a common binary switch that transduces the selected sexual fate, female when ON, male when OFF, to the downstream effector doublesex(dsx) that controls overt sexual differentiation. An interesting recurrent feature is that tra is switched ON in the early zygote by maternally provisioned tra activity. Different male-determining signals evolved, which prevent maternal activation of zygotic tra to allow for male development. In some species, where lack of maternal activation leaves tra in the OFF state, novel female-determining signals were deployed to activate zygotic tra. It appears that both the instructive end of the pathway upstream of tra as well as the executive end downstream of dsx are primary targets of evolutionary divergence, while the transduction part seems less prone to changes. We propose that this is a feature shared with many other signaling cascades that regulate developmental fates.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sex Determination in Insects: Variations on a Common Theme

Bopp

Saccone

Beye

2013

Sex Dev

Self Cite

132

144

View full text Add to dashboard Cite

show abstract

“…The sex-determination pathway can provide novel targets for generating genetic sexing strains [35] for sex-separation and for interference of the sex ratio to reduce mosquito populations. For example, knocking down the female isoform of dsx by RNAi in the larval stage resulted in female lethality [36]; alternative RNAi attempts resulted in reduced female fecundity [37].…”

Section: Sex As a Target For Vector Controlmentioning

confidence: 99%

Control of Mosquito-Borne Infectious Diseases: Sex and Gene Drive

Adelman

2016

Trends in Parasitology

101

View full text Add to dashboard Cite

Sterile male releases have successfully reduced local populations of the dengue vector, Aedes aegypti, but challenges remain in scale and in separating sexes prior to release. The recent discovery of the first mosquito male determining factor (M factor), will facilitate our understanding of the genetic programs that initiate sexual development in mosquitoes. Manipulation of the M-factor and possible intermediary factors may result in female-to-male conversion or female killing, enabling efficient sex-separation and effective reduction of target mosquito populations. Given recent breakthroughs in the development of CRISPR-Cas9 reagents as a source of gene drive, more advanced technologies at driving maleness, the ultimate disease refractory phenotype, become possible and may represent efficient and self-limiting methods to control mosquito populations.

show abstract

“…For sex separation of Tephritids, transgenic sexing strains were developed for different fruit fly genera: (i) an RNA interference (RNAi) system developed for C. capitata based on knockdown of transcripts of the sex determination gene transformer (tra) [30], (ii) lethality systems relying on a simplified single component tetracycline expression system and developed for C. capitata and the olive fruit fly, Bactrocera oleae [31,32], (iii) lethality systems relying on a tetracyclineregulated binary expression system [23], embryonic promoters/enhancers and proapoptotic hid effector, and developed for C. capitata, A. suspensa, and the Mexican fruit fly, Anastrepha ludens [33][34][35]. Unlike the two proof-of-principle transgenic sexing systems based on lethality earlier developed in Drosophila, all the afore-mentioned transgenic sexing systems based on lethality in Tephritids employed the sex-specifically spliced intron of the gene transformer (tra) to confer lethality only to the female individuals.…”

Section: Tephritid Fruit Fliesmentioning

confidence: 99%

Developing the Arsenal Against Pest and Vector Dipterans: Inputs of Transgenic and Paratransgenic Biotechnologies

Ogaugwu¹,

Durvasula²

2017

Biological Control of Pest and Vector Insects

View full text Add to dashboard Cite

Insects are the most numerous of all animals and are found in almost every inhabitable place on earth. The order Diptera (true flies) contains many members that are notorious agricultural pests, nuisance or vectors of diseases. The list is long: mosquitoes, tsetse flies, screw worms, fruit flies, sand flies, blow flies, house flies, gall and biting midges, black flies, leaf miners, horse flies, and so on. Efforts to combat some of these pests and vectors have resulted in control measures such as the chemical, physical, and cultural control methods. These methods, though largely beneficial, have disadvantages and limitations, which sometimes seem to outweigh the problems initially sought to be controlled. The chemical method, for example, is not environment-friendly since it negatively affects many nontarget organisms and disrupts ecosystem balance. Development of insecticide resistance by pests/vectors is another concern. Molecular biotechnology has introduced vast arrays of novel ways to tackle pests and disease vectors, as well as improve the potency of existing control methods. This chapter looks at transgenic and paratransgenic biotechnologies and how they have been applied so far to develop and expand the arsenal against dipteran pests and disease vectors. Further, we discuss the advantages, disadvantages, and limitations of these technologies.

show abstract

New Sexing Strains for Mediterranean Fruit Fly Ceratitis capitata: Transforming Females into Males

Cited by 26 publications

References 17 publications

Sex Determination in Insects: Variations on a Common Theme

Sex Determination in Insects: Variations on a Common Theme

Control of Mosquito-Borne Infectious Diseases: Sex and Gene Drive

Developing the Arsenal Against Pest and Vector Dipterans: Inputs of Transgenic and Paratransgenic Biotechnologies

Contact Info

Product

Resources

About