New set of microsatellite markers for the spotted-wing Drosophila suzukii (Diptera: Drosophilidae): A promising molecular tool for inferring the invasion history of this major insect pest

Fraimout, Antoine; Loiseau, Anne; Price, Daniel O.; Xuéreb, Anne; Jean-François, Martin; Vitalis, Renaud; Fellous, Simon; Debat, Vincent; Estoup, Arnaud

doi:10.14411/eje.2015.079

Cited by 20 publications

(23 citation statements)

References 21 publications

(13 reference statements)

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“…Throughout the course of the invasion of D. suzukii , its genetic diversity changed through bottlenecks and admixture events (Fraimout et al., ). A comparison of the host genotype across neutral markers (6–28 microsatellites) and six X‐linked loci in coding and noncoding sequences indicated relatively high intraspecific genetic variation within and between populations in the invaded regions (Adrion et al., ; Bahder, Bahder, Hamby, Walsh, & Zalom, ; Fraimout et al., , ). It is therefore reasonable to assume that there is substantial intraspecific genotypic variation in the invaded populations that can contribute to the variable D. suzukii –parasitoid outcome.…”

Section: Step 3: Understanding Variation In D Suzukii–parasitoid Intmentioning

confidence: 99%

Optimization of native biocontrol agents, with parasitoids of the invasive pest Drosophila suzukii as an example

Kruitwagen

Beukeboom

Wertheim

2018

Evolutionary Applications

115

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The development of biological control methods for exotic invasive pest species has become more challenging during the last decade. Compared to indigenous natural enemies, species from the pest area of origin are often more efficient due to their long coevolutionary history with the pest. The import of these well‐adapted exotic species, however, has become restricted under the Nagoya Protocol on Access and Benefit Sharing, reducing the number of available biocontrol candidates. Finding new agents and ways to improve important traits for control agents (“biocontrol traits”) is therefore of crucial importance. Here, we demonstrate the potential of a surprisingly under‐rated method for improvement of biocontrol: the exploitation of intraspecific variation in biocontrol traits, for example, by selective breeding. We propose a four‐step approach to investigate the potential of this method: investigation of the amount of (a) inter‐ and (b) intraspecific variation for biocontrol traits, (c) determination of the environmental and genetic factors shaping this variation, and (d) exploitation of this variation in breeding programs. We illustrate this approach with a case study on parasitoids of Drosophila suzukii, a highly invasive pest species in Europe and North America. We review all known parasitoids of D. suzukii and find large variation among and within species in their ability to kill this fly. We then consider which genetic and environmental factors shape the interaction between D. suzukii and its parasitoids to explain this variation. Insight into the causes of variation informs us on how and to what extent candidate agents can be improved. Moreover, it aids in predicting the effectiveness of the agent upon release and provides insight into the selective forces that are limiting the adaptation of indigenous species to the new pest. We use this knowledge to give future research directions for the development of selective breeding methods for biocontrol agents.

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Section: Step 3: Understanding Variation In D Suzukii–parasitoid Intmentioning

confidence: 99%

Optimization of native biocontrol agents, with parasitoids of the invasive pest Drosophila suzukii as an example

Kruitwagen

Beukeboom

Wertheim

2018

Evolutionary Applications

115

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“…Bahder et al in particular analysed samples of D. suzukii populations collected in California and Washington and determined that while D. suzukii in the former region had high levels of genetic variation, the latter was highly monomorphic [ 22 ]. Furthermore, Fraimout’s group investigated Hawaiian and Spanish populations by exploiting microsatellite markers, finding a significant level of genetic differentiation [ 23 ]. Although both studies exploited two different sets of microsatellites and tested different populations, the authors were led to similar conclusions: they demonstrated the presence of a specific differentiation process among ancestral and derived populations and suggested that for D. suzukii a genetic analysis approach is valuable not only to better understanding of the evolutionary history of the species, but also to manage its great potential for invasiveness.…”

Section: Introductionmentioning

confidence: 99%

“…Taking into account this aspect, we used a population genetic approach to characterise genetic diversity among D. suzukii individuals collected in different regions of Italy. In order to perform this work a set of 15 microsatellites validated by Fraimout and colleagues [ 23 ] were employed. The current research is the first study that provides new insights on the trend of genetic diversity in Italian populations of D. suzukii .…”

Section: Introductionmentioning

confidence: 99%

Genetic variability in Italian populations of Drosophila suzukii

Tait

Vezzulli

Sassù³

et al. 2017

BMC Genet

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Background Drosophila suzukii is a highly destructive pest species, causing substantial economic losses in soft fruit production. To better understand migration patterns, gene flow and adaptation in invaded regions, we studied the genetic structure of D. suzukii collected across Italy, where it was first observed in 2008. In particular, we analysed 15 previously characterised Simple Sequence Repeat (SSR) markers to estimate genetic differentiation across the genome of 278 flies collected from nine populations.ResultsThe nine populations showed high allelic diversity, mainly due to very high heterozygosity. The high Polymorphism Information Content (PIC) index values (ranging from 0.68 to 0.84) indicated good discrimination power for the markers. Negative fixation index (F IS) values in seven of the populations indicated a low level of inbreeding, as suggested by the high number of alleles. STRUCTURE, Principal Coordinate and Neighbour Joining analysis also revealed that the Sicilian population was fairly divergent compared to other Italian populations. Moreover, migration was present across all populations, with the exception of the Sicilian one, confirming its isolation relative to the mainland.ConclusionsThis is the first study characterising the genetic structure of the invasive species D. suzukii in Italy. Our analysis showed extensive genetic homogeneity among D. suzukii collected in Italy. The relatively isolated Sicilian population suggests a largely human-mediated migration pattern, while the warm climate in this region allows the production of soft fruit, and the associated D. suzukii reproductive season occurring much earlier than on the rest of the peninsula.Electronic supplementary materialThe online version of this article (10.1186/s12863-017-0558-7) contains supplementary material, which is available to authorized users.

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“…The European and Mediterranean Plant Protection Organization (EPPO) has listed Drosophila suzukii on its A2 List and recently added two new drosophilid species, Zaprionus indianus Gupta and Zaprionus tuberculatus Malloch, on its Alert List (EPPO, 2016a). For D. suzukii, the study of microsatellite markers suggests that European populations come from both Asiatic and North American populations (Fraimout et al, 2015(Fraimout et al, , 2017, but the pathways of introduction are still unknown. The sources of introduction in Europe of these three species are unknown, as are their origins.…”

Section: Introductionmentioning

confidence: 99%

“…The sources of introduction in Europe of these three species are unknown, as are their origins. For D. suzukii, the study of microsatellite markers suggests that European populations come from both Asiatic and North American populations (Fraimout et al, 2015(Fraimout et al, , 2017, but the pathways of introduction are still unknown.…”

Section: Introductionmentioning

confidence: 99%

The drosophilid risk on imports

Balmès

Mouttet

2019

EPPO Bulletin

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The introduction and spread of Drosophila suzukii (Diptera, Drosophilidae) in Europe has had a major impact on agricultural production. The origin of this pest is known, but not the relative importance of the main pathways of entry and spread. Drosophilid larvae may be detected during phytosanitary import inspections, and this study attempted to identify 18 larvae, originating from 8 countries, intercepted by phytosanitary inspectors in France (mainland and overseas territories). Given the difficulties in identifying larval stages using morphology, molecular analysis was carried out on the larvae. While the authors expected that the larvae would be mainly from the genus Drosophila, in fact the majority were from the genus Zaprionus (14 specimens, 78% of the analysis). These included Zaprionus indianus and Zaprionus tuberculatus which were found 7 times (39%) and 3 times (17%), respectively. New host plants are recorded for Zaprionus tuberculatus.

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New set of microsatellite markers for the spotted-wing Drosophila suzukii (Diptera: Drosophilidae): A promising molecular tool for inferring the invasion history of this major insect pest

Cited by 20 publications

References 21 publications

Optimization of native biocontrol agents, with parasitoids of the invasive pest Drosophila suzukii as an example

Optimization of native biocontrol agents, with parasitoids of the invasive pest Drosophila suzukii as an example

Genetic variability in Italian populations of Drosophila suzukii

The drosophilid risk on imports

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