New separation method for monoclonal immunoradiometric assays and its application to assays for thyrotropin and human choriogonadotropin.

Rattle, S J; Purnell, D R; Williams, Pierre-Marc; Siddle, Kenneth; Forrest, G C

doi:10.1093/clinchem/30.9.1457

Cited by 55 publications

(8 citation statements)

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“…Analysis of serum PRL and CORT concentrations was carried out blind to patient status in the Department of Clinical Biochemistry, King's College Hospital. The prolactin was assayed using the immunoradiometric method MAIAClone from Serano (Hampshire, UK) described by Rattle et al (1984), and CORT was assayed using the Coat-A-Coat radioimmunoassay from DPC (California, USA). The inter-assay coefficient of variance was less than 6% for both assays.…”

Section: Methodsmentioning

confidence: 99%

Reduced Prolactin and Cortisol Responses to d-Fenfluramine in Depressed Compared to Healthy Matched Control Subjects

Cleare

Murray²,

O’Keane³

1996

Neuropsychopharmacology

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d-Fenfluramine, a specific 5-HT releasing agent without the catecholamine effects of d,l-fenfluramine, was used as a neuroendocrine challenge in 19 subjects with major depression and 19 healthy controls. Patients and controls were matched for age, sex, weight, and menstrual status. 5-HT-mediated prolactin and cortisol responses were both significantly attenuated in the depressed group. Patients with a history of a suicide attempt had lower cortisol responses than those without. Peak cortisol responses were inversely related to baseline cortisol levels. There were also significant relationships between hormone responses and both age and weight. These findings replicate those of a previous study using this challenge and reiterate the role of reduced 5-HT activity in suicide. They also reinforce the need for careful matching in neuroendocrine studies.

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Section: Methodsmentioning

confidence: 99%

Reduced Prolactin and Cortisol Responses to d-Fenfluramine in Depressed Compared to Healthy Matched Control Subjects

Cleare

Murray²,

O’Keane³

1996

Neuropsychopharmacology

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“…The results are expressed as mi.u./ml of standards calibrated against the WHO 1st IRP for human LH (68/40). The use of high-affinity monoclonal antibodies to FSH and LH offers an increased level of sensitivity and specificity compared with traditional methods (Soos & Siddle, 1983;Rattle et al, 1984). Oestradiol-17ß was measured in serum by a direct radioimmunoassay using a kit purchased from Biodata (Serono Diagnostics Ltd).…”

Section: Methodsmentioning

confidence: 99%

Effects of supraphysiological concentrations of progesterone on the characteristics of the oestradiolinduced gonadotrophin surge in women

Messinis

Templeton²

1990

Reproduction

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Intramuscular injections of oestradiol benzoate were given to 8 normally cyclic women in the early follicular phase of 3 different cycles. Progesterone was also injected in the second (low dose) and the third cycle (high dose). Oestradiol induced simultaneous surges of LH and FSH in all women and the onset of these surges was advanced by progesterone. Low-dose progesterone induced a significant increase in the amplitude and the duration of the LH and FSH surges, while high-dose progesterone decreased the duration significantly. In contrast to the oestrogen-only treatment cycles, when the women were treated with progesterone, basal LH and FSH concentrations were suppressed significantly not only before the onset but also after the end of the surge. The results suggest that progesterone affects the dimension of the oestradiol-induced gonadotrophin surge by exerting both a stimulatory and an inhibitory effect on pituitary gonadotrophin secretion. Supraphysiological concentrations of progesterone decreased the duration of the oestradiol-induced gonadotrophin surge significantly and this is possibly part of the mechanism which attenuates the endogenous LH surge in women superovulated for in-vitro fertilization.

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“…Serum and amniotic fluid TSH concentrations were determined by a double monoclonal RIA (Rattle et al, 1984); fT4 and T3 were also determined by RIA, as previously described (Wilkins et al, 1985). Tanner's and Nellhause charts were used for assessing height and head circumference growth parameters respectively; whereas psychomotor achievement testing was performed according to Gesell and Amatruda's developmental schedules (Gesell, 1979).…”

Section: Patient a N D Methodsmentioning

confidence: 99%

Congenital hereditary hypothyroidism—prenatal diagnosis and treatment

et al. 1990

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Intrauterine diagnosis of congenital hypothyroidism was established on the basis of TSH concentration in amniotic fluid in the 22nd week of gestation for the offspring of a couple both known to have an iodide organification defect. Prenatal treatment consisted of intramniotic injections of 500 mcg Na-1-thyroxine, which was administered from the first amniocentesis until one week before delivery. Following delivery, the diagnosis was confirmed by the elevated level of TSH, 60.5 uU/ml, and a gradual decrease of fT4 to 0.8 ng/ml. Regular substitution therapy was commenced on the third day of life. The normal shape and location of the thyroid gland was demonstrated by Technetium scintiscan. At 18 months the infant revealed no significant deviation from normalcy in growth or mental capacity. This experience indicates that testing of amniotic fluid for TSH in the 22nd week of gestation can be diagnostic for congenital primary hypothyroidism. Furthermore, it is suggested that the treatment approach described is warranted in all cases in which there is a high risk of congenital primary hypothyroidism.

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New separation method for monoclonal immunoradiometric assays and its application to assays for thyrotropin and human choriogonadotropin.

Cited by 55 publications

References 0 publications

Reduced Prolactin and Cortisol Responses to d-Fenfluramine in Depressed Compared to Healthy Matched Control Subjects

Reduced Prolactin and Cortisol Responses to d-Fenfluramine in Depressed Compared to Healthy Matched Control Subjects

Effects of supraphysiological concentrations of progesterone on the characteristics of the oestradiolinduced gonadotrophin surge in women

Congenital hereditary hypothyroidism—prenatal diagnosis and treatment

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