New semen freezing method for chicken and drake using dimethylacetamide as the cryoprotectant

Abstract

The aim is to optimize the dimethylacetamide ( DMA ) straw freezing technology of Black silkies rooster semen through the handy patent equipment, screening the formula of freezing basic extender and optimizing the DMA addition method, and then by comparing the fertility of DMA straw frozen semen with the pellet frozen semen. After the DMA straw freezing technology is optimized, it is extended to the Youxian Partridge drake semen. The result showed that the frozen sperm motility of Lake and Ravie ( LR ) group is 64%, the fertility 49.57% and the hatchability 91.52%, all of which are superior to those of FEB, Beltsville Poultry Semen Extender ( BPSE ) and Lake ( P < 0.05). The sperm motility of adding DMA stock solution is 59%, which is superior to adding DMA directly into diluted semen ( P > 0.05). The fertility and hatchability of DMA straw group are 77.61% and 92.30%, respectively, and it is significantly higher than those in the pellet group ( P < 0.01; P < 0.05). The fresh drake sperm motility of induction collection method is 71%, the massage collection method 61% and the frozen drake sperm motility of induction 33% while the massage 19%. The fertility of frozen drake semen group is 85.93%, while that of the fresh semen group is 88.17%. The frozen drake semen fertility of the highest batch is 93.8%. In conclusion, the world's advanced fertility of frozen semen can be obtained both in the chicken and drake through the optimized DMA straw freezing technology and the method of screening freeze-resistant individuals.

Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen

“…Meanwhile, glycerol has contraceptive action. It can lead to a significant reduction in fertility, which requires the removal of glycerol after thawing before artificial insemination ( Tang et al, 2021 ). Initially, glycerol removal method involves a systematic stepwise dilution and centrifugation for reducing the glycerol concentration gradually after thawing.…”

Raptors bred in captivity: semen characteristics and assisted reproduction outcome in goshawk (Accipiter gentilis)

“…However, some of these techniques cannot guarantee the high success rate achieved by the cooperative method because these are stressful to the birds and involve the capture and manipulation of non-sedated birds. The influence of the semen collection method on the quality of the fresh semen has been clearly demonstrated in a number of avian species ( Castillo, Marzoni & Romboli, 2009 ; Łukaszewicz, Kowalczyk & Rzońca, 2011 ; Castillo et al, 2021 ; Tang et al, 2021 ). The massage method, the most common approach for semen collection ( Łukaszewicz, Kowalczyk & Rzońca, 2015 ; Dogliero et al, 2016 ; Piacek et al, 2020 ), may also stimulate urination and/or blood emission in the case that the handler’s dexterity is insufficient, with the consequent contamination of the ejaculate ( Madeddu et al, 2009 ).…”

Effect of Dimethylacetamide Concentration on Motility, Quality, Antioxidant Biomarkers, Anti-Freeze Gene Expression, and Fertilizing Ability of Frozen/Thawed Rooster Sperm

“…The results of post-thawed sperm motility were appropriate around the level of 6% DMA concentration in different poultry species [ 21 , 22 ]. However, mean fertility obtained with cryopreserved chicken semen with DMA remains low and variable, ranging from 10% to 77% [ 10 , 20 , 23 ].…”