New selective thiolate gold(<scp>i</scp>) complexes inhibit the proliferation of different human cancer cells and induce apoptosis in primary cultures of mouse colon tumors

Abás, Elisa; Pena-Martinez, Raquel; Aguirre-Ramírez, Diego; Rodríguez‐Diéguez, Antonio; Laguna, Mariano; Grasa, Laura

doi:10.1039/c9dt04423j

Cited by 20 publications

(24 citation statements)

References 61 publications

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“…values were higher than other reported for gold(I) complexes [46,47,65], but similar to the documented interactions for potential drugs based on ruthenium or nickel [66,67]. Therefore, our complexes can be transported by BSA protein throughout the body, one molecule each of BSA according to the results of n parameter (except for C1 or C5, where two molecules of gold complex could be found in the same BSA structure).…”

Section: Bovine Serum Albumin Interactionsupporting

confidence: 81%

“…According to the K b values observed in Table 3, there was a moderated interaction force between complex-BSA. These values were higher than other reported for gold(I) complexes [46,47,65], but similar to the documented interactions for potential drugs based on ruthenium or nickel [66,67].…”

Section: Bovine Serum Albumin Interactionsupporting

confidence: 79%

“…This differentiation process is growth-dependent, where the cells undergo differentiation from “undifferentiated proliferative crypt-type cells” in the exponential phase of growth, to “differentiated enterocyte-type cells” in the stationary phase [ 45 ]. The cell culture of Caco-2/TC7 cells (passages 30–50) was performed according to a previously reported method [ 46 , 47 ].…”

Section: Methodsmentioning

confidence: 99%

“…Cell survival was measured using the MTT test [ 48 ]. The assay depends on the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, Madrid, Spain) by the mitochondrial dehydrogenase of viable cells to a blue formazan product that can be measured spectrophotometrically, as previously described [ 46 , 47 ].…”

Section: Methodsmentioning

confidence: 99%

“…Cell homogenates needed for this assay were prepared according to a previously described method [ 47 ]. Protein oxidation was analyzed by carbonyl-level measurement as previously described [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

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Selective Anticancer and Antimicrobial Metallodrugs Based on Gold(III) Dithiocarbamate Complexes

et al. 2021

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New dithiocarbamate cycloaurated complexes have been synthesized and their physicochemical and in vitro antitumor properties have been evaluated. All the performed studies highlighted good transport through the blood and biodistribution, according to the balance between the properties of hydrophilicity/lipophilicity and the binding of moderate strength to the BSA protein. Furthermore, none of the complexes exhibited reduction or decomposition reactions, presenting excellent physiological stability. The in vitro cytotoxic effect was evaluated on human colon cancer cell line Caco-2/TC7, and the complexes showed great antiproliferative activity and excellent selectivity, as much less effect was detected on normal Caco-2/TC7 cells. Most of the complexes exhibit antiproliferative activity that was better than or similar to auranofin, and at least nine times better than that of cisplatin. Its action mechanism is still under discussion since no evidence of cell cycle arrest was found, but an antioxidant role was shown for some of the selective complexes. All complexes were also tested as antimicrobial drugs, exhibiting good activity towards S. aureus and E. coli. bacteria and C. albicans and C. neoformans fungi.

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