New selective media for<i>pseudomonas</i>strains producing fluorescent pigment

Katoh, Kunihiko; Itoh, Kunio

doi:10.1080/00380768.1983.10434655

Cited by 74 publications

(50 citation statements)

References 9 publications

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“…Indigenous and genetically modified Pseudomonas populations were estimated for each sample by preparing from a well mixed, macerated 1 g root sample a 10-fold dilution range in 1/4 strength Ringer's solution. Aliquots of 0·1 ml of appropriate dilutions were plated onto fluorescent Pseudomonas selective agar (Katoh and Itoh 1983) amended with 50 mg ml −1 X-gal. The plates were incubated for 7 d at 25°C and blue (recombinants) and white fluorescent colonies (indigenous Pseudomonas) were enumerated on each plate.…”

Section: Microbial Populationsmentioning

confidence: 99%

Plant mediated interactions between Pseudomonas fluorescens, Rhizobium leguminosarum and arbuscular mycorrhizae on pea

Andrade

Leij

Lynch

1998

Letters in Applied Microbiology

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G . A ND R AD E, F .A .A . M. DE L EI J A N D J .M . LY NC H . 1998. Using undisturbed sandy loam soil cores heavily infested with mycorrhizae, the effects of the antibiotic-producing Pseudomonas fluorescens strain F113 and its non-antibiotic derivative Ps. fluorescens F113G22 on nodulation by introduced and indigenous Rhizobium were studied. Furthermore, the effects of the different microbial inocula on the colonization of the pea roots by mycorrhizae were studied. It was found that Ps. fluorescens F113 enhanced nodulation by Rhizobium fourfold, while the nodules produced were much larger and strongly pigmented (pink) compared with those in other treatments. The proportion of roots colonized by arbuscular mycorrhizae was not significantly affected by the different treatments.

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Section: Microbial Populationsmentioning

confidence: 99%

Plant mediated interactions between Pseudomonas fluorescens, Rhizobium leguminosarum and arbuscular mycorrhizae on pea

Andrade

Leij

Lynch

1998

Letters in Applied Microbiology

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“…Four strains (Ps 1-2, Ps 1-10, Ps 5-5 and Ps 9-14 T ) from soils of the Goesan, Samchok and Umsong regions, three strains (Ps 2-22, Ps 3-1 and Ps 3-10 T ) from soil of Umsong Region and four strains (Pss 14, Pss 25, Pss 26 T and Pss 27) from soil of Jinju Region in Korea were isolated using P1 agar medium (l 21 : 1 g KH 2 PO 4 , 0?5 g MgSO 4 .7H 2 O, 0?2 g KCl, 5 g NaNO 3 , 1 g deoxycholic acid, 5 g betaine and 15 g agar; Kato & Itoh, 1983). In general, all strains were cultured on trypticase soy agar (TSA) medium at 30˚C unless otherwise stated.…”

mentioning

confidence: 99%

Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea

Kwon

Kim

Park

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

195

106

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“…Filamentous fungal populations were then quantified by plating a ten fold dilution series onto 10% malt extract agar (100 ppm streptomycin and 50 ppm rose bengal) plates following incubation at 20°C for 5 days before enumeration. P1 medium 16 (amended with 50 ppm X-Gal as appropriate) incubated at 25°C for 5 days was used for the enumeration of fluorescent Pseudomonas species.…”

Section: Transformation Of Sbw25mentioning

confidence: 99%

Survival and Ecological Fitness of Pseudomonas fluorescens Genetically Engineered with Dual Biocontrol Mechanisms

et al. 2004

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The antibiotic, 2, 4-diacetylphloroglucinol (Phl), is produced by a range of naturally occurring fluorescent pseudomonads. One isolate, P.fluorescens F113, protects pea plants from the pathogenic fungus, Pythium ultimum, by reducing the number of pathogenic lesions on plant roots but with a concurrent reduction in the emergence of plants such as pea. The genes responsible for Phl production have been shown to be functionally conserved between the wild type (wt) P. fluorescens strains F113 and Q2-87. In this study the genes from F113 were isolated using an optimised long PCR method and a 6.7-kb gene cluster inserted into the chromosome of the non-Phl producing P. fluorescens strain SBW25 EeZY6KX. This strain is a lacZY, km R marked derivative of the wild type SBW25 which effects biological control against the plant pathogen, Pythium ultimum by competitive exclusion as a result of its strong rhizosphere colonising ability. We describe here the integration of the Phl antifungal and competitive exclusion mechanisms into a single strain, and the impact this has on survival and plant emergence in microcosms. The insertion of the Phl biosynthetic genes from the F113, into the SBW25 chromosome gave a Phl-producing transformant (strain Pa21) able to suppress P. ultimum through antibiotic production. The growth of Pa21 was not reduced in flask culture at 20 o C, compared with its parent strain. When inoculated on pea seedlings, the strain containing the Phl operon behaved similarly to the SBW25 EeZY6KX parent but did not show the tendency of the wt Phl producer F113 to cause lower pea seed emergence. SBW25 EeZY6KX has significantly lower indigenous populations in the root than the F113 and control. This is indicative of this strain's strong colonising presence. Pa21, the Phl-modified strain is able to exclude the resident population from roots to the same degree as the SBW25 EeZY6KX from which it is derived. This suggests that it has maintained its competitiveness around the root systems of plants even with the introduction of the Phl locus. Thus, strain Pa21 possesses the qualities necessary to provide effective

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New selective media forpseudomonasstrains producing fluorescent pigment

Cited by 74 publications

References 9 publications

Plant mediated interactions between Pseudomonas fluorescens, Rhizobium leguminosarum and arbuscular mycorrhizae on pea

Plant mediated interactions between Pseudomonas fluorescens, Rhizobium leguminosarum and arbuscular mycorrhizae on pea

Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea

Survival and Ecological Fitness of Pseudomonas fluorescens Genetically Engineered with Dual Biocontrol Mechanisms

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