New role of a histone chaperone, HirA: Involvement in kojic acid production associated with culture conditions in Aspergillus oryzae

Kudo, Hayato; Arakawa, Gen-ya; Shirai, Shinnichi; Ogawa, Mitsuhiro; Shindo, Hitoshi; Hosaka, Masaru; Tokuoka, Masafumi

doi:10.1016/j.jbiosc.2021.12.001

Cited by 8 publications

(2 citation statements)

References 33 publications

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“…Wei et al reported that the production of validamycin A ( 25 ) by Streptomyces hygroscopicus 5008 could be activated at the transcriptional level by simply adding hydrogen peroxide (H 2 O 2 ; which generates ROS) to the culture medium [ 96 ]. In addition, there are also reports of cases in which denatured proteins are folded by molecular chaperones in high-temperature environments, and such activity may affect the production of secondary metabolites [ 97 ]. Saito et al are currently attempting to determine how HSMs are produced.…”

Section: Reviewmentioning

confidence: 99%

Methodology for awakening the potential secondary metabolic capacity in actinomycetes

Saito,

Arai

2024

Beilstein J. Org. Chem.

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Secondary metabolites produced by actinomycete strains undoubtedly have great potential for use in applied research areas such as drug discovery. However, it is becoming difficult to obtain novel compounds because of repeated isolation around the world. Therefore, a new strategy for discovering novel secondary metabolites is needed. Many researchers believe that actinomycetes have as yet unanalyzed secondary metabolic activities, and the associated undiscovered secondary metabolite biosynthesis genes are called “silent” genes. This review outlines several approaches to further activate the metabolic potential of actinomycetes.

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Section: Reviewmentioning

confidence: 99%

Methodology for awakening the potential secondary metabolic capacity in actinomycetes

Saito,

Arai

2024

Beilstein J. Org. Chem.

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“…Kojic acid has great application value in such fields as the food, cosmetic, pharmaceutical and agriculture industries, and is a secondary metabolite produced by Aspergillus oryzae [ 1 , 2 , 3 ]. Some progress has been made in studying the mechanism underlying kojic acid synthesis [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. The global transcriptional regulator of the secondary metabolite laeA negatively mediates kojic acid production through the regulation of the kojic acid synthesis genes kojA , kojR and kojT [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Major Facilitator Superfamily Transporter Gene Aokap4 near the Kojic Acid Gene Cluster Is Involved in Growth and Kojic Acid Production in Aspergillus oryzae

Chen

et al. 2022

JoF

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Kojic acid is an important secondary metabolite of industrial importance produced by Aspergillus oryzae. The kojic acid gene cluster plays an essential role in kojic acid production, and harbors kojA, kojR and kojT. The deletion of kojT, encoding a major facilitator superfamily (MFS) transporter, did not completely abolish kojic acid production, implying that other transporters are required for the transport of kojic acid. The aim of this study is to look for the transporters involved in kojic acid production. Here, Aokap4 encoding a novel MFS transporter was identified, which was adjacent to kojT in the kojic acid gene cluster. The deletion of Aokap4 contributed to the hyphal growth, conidial production and biomass of A. oryzae. Moreover, Aokap4 is required for heat- and cell-wall-stress tolerance but not oxidative and osmotic stress. The disruption of Aokap4 impaired kojic acid production with the reduced expression of kojA, kojR and kojT. Furthermore, when kojT was deleted in the Aokap4-disrupted strain, the yield of kojic acid declined to the same level as that of the kojT-deletion mutant, whereas the production of kojic acid was recovered when kojT was overexpressed in the Aokap4 knockout strain, suggesting that kojT acts downstream of Aokap4. AoKap4 was the second identified MSF transporter involved in kojic acid production after kojT was found a decade ago. This study contributes to a better understanding of the biological roles of the MFS transporter and lays a foundation for future studies on kojic acid synthesis in A. oryzae.

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Disruption of Aokap6 near the kojic acid gene cluster affects the growth and kojic acid production in Aspergillus oryzae

Chen

Chen²,

Wang³

et al. 2022

World J Microbiol Biotechnol

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New role of a histone chaperone, HirA: Involvement in kojic acid production associated with culture conditions in Aspergillus oryzae

Cited by 8 publications

References 33 publications

Methodology for awakening the potential secondary metabolic capacity in actinomycetes

Methodology for awakening the potential secondary metabolic capacity in actinomycetes

A Novel Major Facilitator Superfamily Transporter Gene Aokap4 near the Kojic Acid Gene Cluster Is Involved in Growth and Kojic Acid Production in Aspergillus oryzae

Disruption of Aokap6 near the kojic acid gene cluster affects the growth and kojic acid production in Aspergillus oryzae

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