New records of Saprolegniaceae isolated from rainbow trout, from their eggs, and water in a fish farm from the State of México

Vega-Ramírez, Ma. Teresa; Moreno-Lafont, Martha; Valenzuela, Ricardo; Cervantes-Olivares, Roberto; Aller-Gancedo, J. Miguel; Fregeneda-Grandes, Juan-Miguel; Damas-Aguilar, José L.; García-Flores, Valeria; López-Santiago, Rubén

doi:10.7550/rmb.28627

Cited by 18 publications

(16 citation statements)

References 7 publications

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“…is the most common fungal disease affecting fish and their eggs in fresh and brackish water (Eli et al., ). This disease causes serious losses in fish farms and hatcheries and is a key disease in farmed and wild fish in many countries (Ke et al., ; Karouei, ; Albert et al., ; Vega‐Ramírez et al., ). The hyphae grow through the epidermis and dermis into the musculature and usually cause dermatitis and myositis (Hussein and Hatai, ).…”

Section: Introductionmentioning

confidence: 99%

The in vitro antifungal activity of 30 Chinese herb extracts to Saprolegnia sp.

et al. 2015

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Chinese traditional medicines have been used for several thousands of years in Asian countries, not only in humans but also in many animal species. These compounds prevent and control different types of diseases including internal diseases as well as some infectious diseases, where the aetiological agent is viral, bacterial, parasitic or mycotic. Rhizoma coptidis is believed to inhibit Shigella dysenteriae and that Radix isatidis can prevent flu caused by the influenza virus. It is thus hypothesized that some of these traditional herbal compounds will have anti-fungal activity. Saprolegniosis is a disease common in fish and their eggs in both fresh and brackish water; a newer, safer medication against Saprolegnia is needed after the prohibition in many countries of the extremely effective fungicide, malachite green. In the present study an attempt is made to identify herbal compounds that have anti-Saprolegnia activity. A strain of Saprolegnia, CCF1301, was isolated from the skin of infected grass carp (Ctenopharyngodon idella) and identified as Saprolegnia ferax by the 26S rDNA D1/D2 region and ITS region. This strain was used to evaluate the antifungal activity of thirty Chinese traditional herbal medicine extracts, and a modified dish dilution method was developed for the evaluation. Saprolegnia-infected rapeseeds with visible hyphae were transplanted onto prepared PDA plates containing 2 g L À1 herbal plant extracts and incubated at 20°C for 48 h. Each herbal plant species was tested in triplicate. Those herbal plant extracts that showed negative mycelium presence at 2 g L À1 were further tested for minimum inhibitory concentration (MIC) evaluation. The results showed that Syzygium aromaticum, Magnolia officinalis, Melaphis chinensis, Euphorbia fischeriana Steud, and Sophora flavescentis exhibited enhanced growth inhibition at 2 g L À1 and MIC values of 500, 62.5, 250, 62.5, 250 mg L À1 concentrations, respectively. It was obvious that Magnolia officinalis and Euphorbia fischeriana Steud exhibited the best antifungal activity. Since there is a high natural toxicity in Euphorbia fischeriana Steud, its applicability as the main ingredient in an aquaculture therapeutic formulation requires further research. Thus, Magnolia officinalis would appear to be the more valuable antifungal herbal species with which to pursue further research.

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Section: Introductionmentioning

confidence: 99%

The in vitro antifungal activity of 30 Chinese herb extracts to Saprolegnia sp.

et al. 2015

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“…In the embryonic stage, the typical symptom is the outgrowth of "cotton like" mycelium (Fernández-Benéitez et al, 2008;Rezinciuc et al, 2014). Currently, the lack of an effective chemical control has resulted in a rapid increase in the number of reported cases of saprolegniosis in fish farms (Cao et al, 2012;Fregeneda-Grandes et al, 2007;Ghiasi et al, 2010;Rezinciuc et al, 2014;Thoen et al, 2011;Vega-Ramírez et al, 2013). In the past the consequence of this disease was generally discounted because it was easily controlled with malachite green (Bailey, 1984;Robertson et al, 2009;van West, 2006).…”

Section: Introductionmentioning

confidence: 99%

Saprolegnia species affecting the salmonid aquaculture in Chile and their associations with fish developmental stage

et al. 2014

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The rapid increase in the aquaculture production of salmonids has been followed by a rise in several diseases. In particular, saprolegniosis can account for at least 10% of the annual economic loss in salmonids. In this study, we investigated the main Saprolegnia species involved in saprolegniosis of salmonids in Chile, and their association with specific developmental stages of the host fish. For this purpose, we studied 244 isolates of Saprolegniaaffected Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), and king salmon (Oncorhynchus tshawytscha) from the salmon farming regions, using a recently developed identification strategy based on molecular taxonomical operational units. We found that the Saprolegnia species associated with diseased salmon were Saprolegnia australis, Saprolegnia delica, Saprolegnia diclina, Saprolegnia ferax, Saprolegnia parasitica and two new Saprolegnia species observed during this study. In order to determine whether there were any specific species associations with different stages in the fish life cycle, we applied mosaic plots and correspondence analyses for categorical data. These analyses showed a strong association of S. parasitica with samples from the adult stage of the fish (χ 2 = 196.29, p b 0.0001), while the species S. australis, S. diclina and Saprolegnia sp. 2 were strongly associated with embryonic stages (eggs or alevins) (χ 2 = 196.29, p b 0.0001). This work represents the first detailed molecular characterization of Saprolegnia species involved in saprolegniosis in Chile, and the first study showing specific association of different Saprolegnia species with different stages in the salmonid life cycle.

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“…The sexual reproduction in oomycete is usually accomplished when the antheridial cell enters into the oögonium through a fertilization tube or cytoplasmic contact and fertilizes an oösphere. However, the identification and classification of Saprolegnia are problematic even after molecular characterization by different techniques due to the morphology of asexual zoosporangia and the way to produce spores, which determine the delineation of genera in the Saprolegniales (Vega-Ramírez et al 2013 ). In the present study, BODIPY 505/515nm was used as a lipophilic bright greenish fluorescent dye that required a minimum staining time of 5 min with low quantity of dye.…”

Section: Resultsmentioning

confidence: 99%

Potential of aquatic oomycete as a novel feedstock for microbial oil grown on waste sugarcane bagasse

Patel

Μάτσακας

Pruthi

et al. 2018

Environ Sci Pollut Res

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Biodiesel production from vegetable oils is not sustainable and economical due to the food crisis worldwide. The development of a cost-effective non-edible feedstock is essential. In this study, we proposed to use aquatic oomycetes for microbial oils, which are cellulolytic fungus-like filamentous eukaryotic microorganisms, commonly known as water molds. They differ from true fungi as cellulose is present in their cell wall and chitin is absent. They show parasitic as well as saprophytic nature and have great potential to utilize decaying animal and plant debris in freshwater habitats. To study the triacylglycerol (TAG) accumulation in the aquatic oomycetes, the isolated water mold Achlya diffusa was cultivated under semi-solid-state conditions on waste sugarcane bagasse, which was compared with the cultivation in Czapek (DOX) medium. A. diffusa grown on waste sugarcane bagasse showed large lipid droplets in its cellular compartment and synthesized 124.03 ± 1.93 mg/gds cell dry weight with 50.26 ± 1.76% w/w lipid content. The cell dry weight and lipid content of this water mold decreased to 89.54 ± 1.21 mg/gds and 38.82% w/w, respectively, when cultivated on standard medium Czapek-Dox agar (CDA). For the fatty acid profile of A. diffusa grown in sugarcane bagasse and CDA, in situ transesterification (IST) and indirect transesterification (IDT) approaches were evaluated. The lipid profile of this mold revealed the presence of C12:0, C14:0, C16:0, C18:0, C18:1, C18:2, C20:0, and C21:0 fatty acids, which is similar to vegetable oils. The biodiesel properties of the lipids obtained from A. diffusa satisfied the limits as determined by international standards ASTM-D6751 and EN-14214 demonstrating its suitability as a fuel for diesel engines.

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New records of Saprolegniaceae isolated from rainbow trout, from their eggs, and water in a fish farm from the State of México

Cited by 18 publications

References 7 publications

The in vitro antifungal activity of 30 Chinese herb extracts to Saprolegnia sp.

The in vitro antifungal activity of 30 Chinese herb extracts to Saprolegnia sp.

Saprolegnia species affecting the salmonid aquaculture in Chile and their associations with fish developmental stage

Potential of aquatic oomycete as a novel feedstock for microbial oil grown on waste sugarcane bagasse

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