New Records of Lasiodiplodia theobromae in Seeds of Tetrapleura tetraptera from Nigeria and Fruit of Cocos nucifera From Mexico

Dugan, Frank M.; Lupien, S. L.; Osuagwu, A. N.; Uyoh, Edak Aniedi; Okpako, E. C.; Kisha, Theodore J.

doi:10.1111/jph.12384

Cited by 7 publications

(7 citation statements)

References 10 publications

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“…Endophytic Fusarium strains have previously been found to promote germination and seedling growth of an Indonesian peatland grass ( Tamura et al, 2008 ) and germination of orchid seeds ( Bayman and Otero, 2007 ). In addition to the aforementioned roles of L. theobromae in tropical fruit tree diseases, it has also been implicated in seed rot, for instance of slash pine ( Cilliers et al, 1993 ), and to cause reduced germination rates in aridan and coconut seeds ( Dugan et al, 2016 ). The role of seedborne L. theobromae on germination may be more nuanced, however, as it has been found to produce fatty acid esters, which can alternately inhibit and promote tobacco seed germination and seedling growth ( Uranga et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives

et al. 2021

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Seed banks were first established to conserve crop genetic diversity, but seed banking has more recently been extended to wild plants, particularly crop wild relatives (CWRs) (e.g., by the Millennium Seed Bank (MSB), Royal Botanic Gardens Kew). CWRs have been recognised as potential reservoirs of beneficial traits for our domesticated crops, and with mounting evidence of the importance of the microbiome to organismal health, it follows that the microbial communities of wild relatives could also be a valuable resource for crop resilience to environmental and pathogenic threats. Endophytic fungi reside asymptomatically inside all plant tissues and have been found to confer advantages to their plant host. Preserving the natural microbial diversity of plants could therefore represent an important secondary conservation role of seed banks. At the same time, species that are reported as endophytes may also be latent pathogens. We explored the potential of the MSB as an incidental fungal endophyte bank by assessing diversity of fungi inside stored seeds. Using banana CWRs in the genus Musa as a case-study, we sequenced an extended ITS-LSU fragment in order to delimit operational taxonomic units (OTUs) and used a similarity and phylogenetics approach for classification. Fungi were successfully detected inside just under one third of the seeds, with a few genera accounting for most of the OTUs–primarily Lasiodiplodia, Fusarium, and Aspergillus–while a large variety of rare OTUs from across the Ascomycota were isolated only once. Fusarium species were notably abundant–of significance in light of Fusarium wilt, a disease threatening global banana crops–and so were targeted for additional sequencing with the marker EF1α in order to delimit species and place them in a phylogeny of the genus. Endophyte community composition, diversity and abundance was significantly different across habitats, and we explored the relationship between community differences and seed germination/viability. Our results show that there is a previously neglected invisible fungal dimension to seed banking that could well have implications for the seed collection and storage procedures, and that collections such as the MSB are indeed a novel source of potentially useful fungal strains.

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Section: Discussionmentioning

confidence: 99%

Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives

et al. 2021

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“…En algunos casos ha sido posible asociar síntomas con la especie del patógeno debido a ciertas características como la presencia de anillos en las lesiones (Tian et al, 2018). Los síntomas asociados a estos hongos son: cancro en ramas y tronco, declinamiento, gomosis, muerte descendente de ramas, necrosis en hojas, pudriciones de semillas, frutos, pedúnculo, raíz y tizón de brotes e inflorescencias (Slippers et al, 2005;Sandoval-Sánchez et al, 2013;Dugan et al, 2015;Hajlaoui et al, 2015;Netto et al, 2017;Rodríguez-Gálvez et al, 2017).…”

Section: Síntomasunclassified

Botryosphaeriaceae: una familia de hongos, compleja, diversa y cosmopolita

Flores¹,

Rodríguez-Alvarado

Gmez-Dorantes

et al. 2021

Remexca

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En la última década se ha incrementado el interés por estudiar los hongos de la familia Botryosphaeriaceae debido a las enfermedades que inducen en cultivos de importancia económica a su amplia distribución cosmopolita y a la asociación observada entre la patogénesis y el estrés del hospedante. Se han reportado más de diez especies asociadas a síntomas en diferentes partes de una misma planta, lo que indica que un número importante de especies de esta familia no presentan especificidad en rango de hospedantes. Además, diversos estudios han demostrado la capacidad de estos hongos para ‘brincar’ de sus hospedantes nativos originales a cultivos agrícolas que se establecen en áreas cercanas, pertenecientes a la misma familia botánica o a una familia diferente. El objetivo de este trabajo es revisar marcadores morfológicos y moleculares para la identificación taxonómica de especies de la familia Botryosphaeriace, su distribución geográfica, rango de hospedantes agrícola y aspectos de desarrollo para la enfermedad incluyendo modos de dispersión. La información presentada podrá ser de utilidad en la etiología, identificación y diagnóstico de especies de Botryosphaeriace así como el manejo de las enfermedades causadas que ellas inducen.

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“…Fabaceae (Linaldeddu et al, 2015), naranjo agrio (Alves et al, 2008), coco (Dugan et al, 2015), mango (Johnson et al, 1992;Marques et al, 2013), papayo (Netto et al, 2014), álamo, sauce (Hashemi & Mohammadi, 2016) guapuruvú (Schizolobium parahyba var. Amazonicum) (Mehl et al, 2014), berenjena (Solanum melongena) (Woodward et al, 2005), níspero (Eriobotrya japonica) (González et al, 2017), yaca (Artocarpus heterophyllus) (Ni et al, 2008), palma (Dypsis lutescens) (Pereira et al, 2015), kenaf (Norhayati et al, 2016), Jatropha (Machado et al, 2014) y varias especies introducidas de árboles forestales, de otros países han facilitado la dispersión de este patógeno (Segura-Contreras et al, 2015).…”

Section: P a L A B R A S C L A V Eunclassified

“…It has also been reported that species of Lasiodiplodia are common in the tropics, causing diseases in plants, in pre and post-harvest and that their cellulolytic activity allows for penetration and colonization of the plant in a similar way to those of a soft rot causing-fungus, using the starch and other saccharides present in the initial substrate of the wood (Muñoz et al, 2015). The imports of other fruit hosts of this pathogen from other countries such as the nut and the English walnut (Li et al, 1995), grape (Linaldeddu et al, 2015;Rodríguez et al, 2015), sweet orange, oak, retama: Fabaceae (Linaldeddu et al, 2015), bitter orange (Alves et al, 2008), coconut (Dugan et al, 2015), mango (Johnson et al, 1992;Marques et al, 2013), papaya (Netto et al, 2014), poplar, willow (Hashemi & Mohammadi, 2016) Brazilian firetree (Schizolobium parahyba var. Amazonicum) (Mehl et al, 2014), eggplant (Solanum melongena) (Woodward et al, 2005), loquat (Eriobotrya japonica) (González et al, 2017), jackfruit (Artocarpus heterophyllus) (Ni et al, 2008), golden cane palm (Dypsis lutescens) (Pereira et al, 2015), kenaf (Norhayati et al, 2016), Jatropha (Machado et al, 2014) and various introduced species of forest trees from other countries have eased the dispersion of this pathogen (Segura-Contreras et al, 2015).…”

Section: P a L A B R A S C L A V Ementioning

confidence: 99%

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2019

rbio

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Muestras de árboles de lima 'Persa', de 5 a 8 años de edad, con síntomas de gomosis, canceres y muerte descendente de ramas fueron colectadas en huertos de los municipios de Tlaltizapán, Ayala, Coatlán del Río y Amayuca en el estado de Morelos, México, durante agosto del 2015. Las muestras fueron procesadas mediante procedimientos de rutina, sembradas en papadextrosa-agar y purificadas mediante puntas de hifa. Los patógenos aislados fueron inoculados en plantas sanas para evaluar su patogenicidad. Se realizó la identificación morfológica y molecular y se construyó un árbol filogenético bayesiano con agrupamiento de clados. Con base a caracteres morfológicos se identificó a Lasiodiplodia sp. El análisis molecular determinó 16 aislados, que se agruparon en 4 clados y tres especies, Lasiodiplodia citricola, L. pseudotheobromae y L. theobromae, las cuales son consideradas como nuevos reportes causando enfermedad en lima 'Persa' para el Estado de Morelos, México. Samples of 'Persian' lime trees, from 5 to 8 years old, with symptoms of gummosis, cankers and dieback of branches were collected in orchards in the municipalities of Tlaltizapan, Ayala, Coatlan del Rio and Amayuca in the state of Morelos, Mexico, during August 2015. The samples were processed by routine procedures and sown in potato dextrose-agar and purified by means of hypha tips. The isolated pathogens were inoculated in healthy plants in order to evaluate their pathogenicity. Morphological and molecular identification was carried out and a Bayesian phylogenetic tree was constructed with clades grouping.

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New Records of Lasiodiplodia theobromae in Seeds of Tetrapleura tetraptera from Nigeria and Fruit of Cocos nucifera From Mexico

Cited by 7 publications

References 10 publications

Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives

Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives

Botryosphaeriaceae: una familia de hongos, compleja, diversa y cosmopolita

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