New Record of Western Flower Thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in South India

Suganthy, M.; Rageshwari, S.; Senthilraja, C.; Nakkeeran, S.; Malathi, V. G.; Ramaraju, K.; Renukadevi, P.

doi:10.22161/ijeab/1.4.33

Cited by 7 publications

(4 citation statements)

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“…T. palmi, S. dorsalis, T. tabaci, and F. schultzei have been reported so far to transmit five tospoviruses such as groundnut bud necrosis virus (GBNV), watermelon bud necrosis virus (WBNV), peanut yellow spot virus (PYSV), capsicum chlorosis virus (CaCV) and Irish yellow spot virus (IYSV) [4]. Recently F. occidentalis, a potential vector of TSWV has been reported from the Nilgiri hills of India [9,10]. Fig.…”

Section: Discussionmentioning

confidence: 99%

“…AG35F-AG36R, AG47F-AG48R, AG87F-AG88R, and AG79F-AG80R for T. palmi, S. dorsalis, T. tabaci, and F. schultzei with the templates of the four thrips vectors separately and mixed templates of all four thrips vectors. Lane 1: 100 bp plus DNA ladder; Lane 2: water control; Lane 3-6: PCR amplicons using species-specific primers with DNA templates of respective thrips vectors, T. palmi (3), S. dorsalis (4), T. tabaci (5), and F. schultzei; Lane 7-11: PCR amplicons using cocktails of primer pairs specific to T. palmi, S. dorsalis, T. tabaci, and F. schultzei with DNA templates of T. palmi (7), S. dorsalis (8), T. tabaci (9), F. schultzei (10), and mixed templates of all four thrips vectors (11). The multiplex PCR using a cocktail of all four thrips vectors amplified products of 568 bp, 713 bp, 388 bp, and 200 bp of T. palmi, S. dorsalis, T. tabaci, and F. schultzei, respectively.…”

Section: Multiplex Pcr Assaymentioning

confidence: 99%

“…Thrips palmi Karny [5,6], Scirtothrips dorsalis Hood [7], T. tabaci Lindeman [8], and Frankliniella schultzei Trybom [7] are reported to transmit five tospoviruses in India [4]. Although F. occidentalis (Pergande) has been recently reported in India [9,10], its distribution and role in spreading tospoviruses under Indian conditions are yet to be studied.…”

Section: Introductionmentioning

confidence: 99%

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A multiplex PCR assay for rapid identification of major tospovirus vectors reported in India

et al. 2020

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Background: To date, four thrips vectors have been reported to transmit five different tospoviruses in India. Their identification at an early stage is crucial in formulating appropriate pest management strategies. Since morphometric key-based thrips identification based on the adult stage is time-consuming, there is a need to develop diagnostic tools which are rapid, accurate, and independent of developmental stages. Here, we report a multiplex PCR assay to identify four major thrips vectors viz. Thrips palmi, T. tabaci, Scirtothrips dorsalis, and Frankliniella schultzei present in India. Results: Cytochrome oxidase subunit III and internal transcribed spacer region 2 were utilized to design speciesspecific primers. Of 38 pairs of primers tested, primer pairs AG35F-AG36R, AG47F-AG48R, AG87F-AG88R, and AG79F-AG80R amplified 568 bp, 713 bp, 388 bp, and 200 bp products from the DNA templates of T. palmi, S. dorsalis, T. tabaci, and F. schultzei, respectively at same PCR conditions. The specificity of the primer pairs was validated with a large number of known specimens and no cross-reactivity was observed with other thrips species. The multiplex PCR assay with a cocktail of all the four primer pairs detected four thrips vectors efficiently and could discriminate all of them concurrently in a single reaction. Conclusion: The multiplex PCR reported in this study could identify the major thrips vectors reported in India. The assay will be useful in ascertaining distribution profile of major thrips vectors, disease epidemiology, screening large samples, and quarantine.

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Section: Discussionmentioning

confidence: 99%

Section: Multiplex Pcr Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A multiplex PCR assay for rapid identification of major tospovirus vectors reported in India

et al. 2020

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“…These markers detected the immature stages as well as adults, even up to 1:120 dilutions without any cross-reactivity to other thrips species [ 55 , 56 ]. In addition to morphology, COI sequences were used to substantiate the incursion of new species, such as H. longisensibilis Xie, Mound, and Zhang in northern Brazil [ 57 ]; and T. parvispinus (Karny) [ 58 ], Podothrips erami Minaei, and F. occidentalis in India [ 59 , 60 ]. Furthermore, strain-specific COI PCR primers were shown to efficiently discriminate strains and reproductive stages of T. tabaci [ 61 ].…”

Section: Pcr-based Identification Of Thrips Using Molecular Markersmentioning

confidence: 99%

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

Ghosh

Jangra

Dietzgen

et al. 2021

Insects

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Thrips are insect pests of economically important agricultural, horticultural, and forest crops. They cause damage by sucking plant sap and by transmitting several tospoviruses, ilarviruses, carmoviruses, sobemoviruses, and machlomoviruses. Accurate and timely identification is the key to successful management of thrips species. However, their small size, cryptic nature, presence of color and reproductive morphs, and intraspecies genetic variability make the identification of thrips species challenging. The use of molecular and electronic detection platforms has made thrips identification rapid, precise, sensitive, high throughput, and independent of developmental stages. Multi-locus phylogeny based on mitochondrial, nuclear, and other markers has resolved ambiguities in morphologically indistinguishable thrips species. Microsatellite, RFLP, RAPD, AFLP, and CAPS markers have helped to explain population structure, gene flow, and intraspecies heterogeneity. Recent techniques such as LAMP and RPA have been employed for sensitive and on-site identification of thrips. Artificial neural networks and high throughput diagnostics facilitate automated identification. This review also discusses the potential of pyrosequencing, microarrays, high throughput sequencing, and electronic sensors in delimiting thrips species.

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The Occurrence, Biology, Serology and Molecular Biology of Tospoviruses in Indian Agriculture

Basavaraj

Mandal

Gawande

et al. 2017

A Century of Plant Virology in India

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New Record of Western Flower Thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in South India

Cited by 7 publications

References 2 publications

A multiplex PCR assay for rapid identification of major tospovirus vectors reported in India

A multiplex PCR assay for rapid identification of major tospovirus vectors reported in India

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

The Occurrence, Biology, Serology and Molecular Biology of Tospoviruses in Indian Agriculture

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