New record forGalba neotropica(d'Orbigny, 1835) in Argentina, with a detailed analysis of its morphology and molecular characteristics

Abstract

2015): New record for Galba neotropica (d'Orbigny, 1835) in Argentina, with a detailed analysis of its morphology and molecular characteristics, Molluscan Research, We report for the first time the presence of Galba neotropica in Santa Fe province, Pampean Argentina. Until the present work, the identity of the intermediate host of Fasciola hepatica in this region, the most important livestock production area of Argentina, was unknown. This report extends the geographic distribution of G. neotropica and is the first to provide molecular and morphological information on this species in Argentina. During summer 2013, snails were collected on private livestock farms with high prevalence of fasciolosis in cattle. Specimens were identified by using the nuclear sequences of the internal transcribed spacers ITS-1 and ITS-2, the shell and the shape and size of the male reproductive organs. Molecularly, ITS-1 and ITS-2 nuclear sequences exhibit 100% nucleotide identity with G. neotropica from Lima, Peru. Morphologically, G. neotropica from Santa Fe province was indistinguishable from Galba viatrix and Galba cubensis, the other two cryptic species within this South American group of lymnaeids. The combination of molecular and morphological analyses is strongly recommended to identify lymnaeids at species level. The identity of the intermediate host and the subsequent knowledge of its susceptibility, behaviour, distribution, ecology and biology are important components in developing effective measures to control fasciolosis.

Frecuencia de fascioliasis en ganado y de Fasciola hepatica en caracoles Lymnaeidae en el distrito de Huanca, Arequipa, Perú

“…En la comunidad de San Basilio no se encontraron ejemplares Lymnaeidae, posiblemente debido a las condiciones medioambientales que estarían limitando su distribución y abundancia; por ello la importancia de contar con estudios ambientales y su influencia en el ciclo biológico de los caracoles. En este sentido, Pujadas et al (2015) en Santa Fe, Argentina, describieron en su estudio las características fisicoquímicas de temperatura (20.6-29 °C), conductividad (0.6-2.3 mS/cm) y pH ligeramente alcalino (7.6-8.3). Asimismo, Iturbe y Muñiz (2012) en su trabajo en Cusco, Perú, determinaron la calidad fisicoquímica de las aguas de los biotopos de los caracoles.…”

“…Las características conquiológicas de otros Lymnaeidae han sido reportados. Así, especímenes de Galba cubensis procedentes del Delta del Ebro en España, presentaron conchillas con dimensiones levemente mayores (alto: 8.1 mm; ancho: 4.7 mm) pero una altura menor (3.8 mm) (Schniebs et al, 2018); caracoles Galba neotropica de Santa Fe, Argentina, mostraron longitudes de conchillas entre 4.1 y 8.5 mm y ancho entre 2.5 y 5.1 mm (Pujadas et al, 2015); asimismo, especímenes de Lymnaea neotropica y L. viator en Catamarca, Argentina, presentaron medidas superiores, además de tener peristoma delgado y ápice de la espira obtuso (Bargues et al, 2016), en tanto que para L. schirazensis se han reportado proporciones similares a las del presente estudio (Bargues et al, 2011). Investigaciones en Perú realizadas por Iturbe y Muñiz (2012) en San Jerónimo, Cusco, han descrito las conchillas de Galba truncatula dimensiones semejantes a los de la Zona del presente estudio (largo: 5.08 mm, ancho: 2.85 mm, ancho: 1.74 mm, largo de la abertura: 2.64 mm) aunque con mayor longitud para el largo de la espira (2.6 mm).…”

First molecular characterization of Galba neotropica in Uruguay

“…For instance, Galba truncatula (= Lymnaea truncatula), the main intermediate host of F. hepatica in Europe, has been also found in distant locations such as the Bolivian Altiplano (Mas-Coma et al, 2001). Moreover, other lymnaeids such as Galba viatrix and Galba neotropica seem to have also a high adaptive behavior, as they were found in diverse environments in Argentina and Chile (Kleiman et al 2007;Mera y Sierra et al 2009;Artigas et al 2011;Standley et al 2013;Pujadas et al 2015). In South America, the most important lymnaeid species involved in the life cycle of F. hepatica seemed to be Pseudosuccinea columella, G. truncatula, G. viatrix, Galba cubensis and G. neotropica (Bargues et al, 2007;Artigas et al, 2011).…”

The first record of Galba cubensis (L. Pfeiffer, 1839) (Mollusca: Gastropoda: Lymnaeidae) from open fields of Europe

“…In 2007, G. neo tropica was described by barGueS et al (2007). It was found in Peru, Argentina, Venezuela, and Uruguay (PuJadaS et al 2015, barGueS et al 2017 and is also an intermediate host for Fasciola hepatica (PuJadaS et al 2015, barGueS et al 2016, 2017. There is still some uncertainty about the possible synonymy of G. cubensis, G. viator andG.…”