New Rapid Assay for Nafcillin by Spectrofluorimetry in Pharmaceutical Dosage

Murillo, J. A.; Alañón, A.

doi:10.1080/00032719308017480

Cited by 8 publications

(11 citation statements)

References 10 publications

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“…Select a suitable trajectory which passes through the excitation and emission maxima of amiloride and obtain matrix isopotential synchronous spectra by means of Ftotal. 10 Calculate the first derivative according to the Savitzky and Golay algorithm. 23,24 Finally, determine the amiloride content by measuring the derivative signal at the emission wavelength of 392.0 nm and using the appropriate calibration graph.…”

Section: Methodsmentioning

confidence: 99%

“…Trajectory A was obtained from the spectrum corresponding to the arithmetic mean of 10 total fluorescence spectra of the different urine samples by means of the Ftotal program. 10 Urine sample solutions containing amiloride gave signals smaller than those obtained with aqueous standard solutions, owing to some type of binding with other components of the urine.…”

Section: Determination Of Amiloride In Urinementioning

confidence: 96%

“…Once the trajectory had been defined, the spectrum was obtained by means of the Ftotal program. 10 The spectra obtained from it displayed the same format as those obtained directly with the Aminco Bowman Series 2 spectrofluorimeter.…”

Section: Softwarementioning

confidence: 99%

“…This trajectory is obtained by means of a program developed in BASIC. 10 It is always possible to find the matrix trajectory that passes through the maximum fluorescence excitation and emission wavelengths of the component to be determined. Therefore, the same sensitivity is achieved as in direct determinations in the absence of background fluorescence.…”

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confidence: 99%

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Direct Determination of Amiloride in Urine Using Isopotential Fluorimetry

Pulgarı́n

Molina²,

López³

1997

Analyst

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A method for the determination of amiloride at concentrations between 15 and 152 ng ml-1 by means of matrix isopotential synchronous fluorescence spectrometry and derivative techniques is proposed. This method is useful for the determination of compounds in samples with unknown background fluorescence without the need for tedious pre-separation. As amiloride is widely used as a doping substance in sport, the method was successfully applied to the determination of amiloride in urine. To obtain maximum sensitivity and adequate selectivity, factors affecting fluorescence intensity were studied in the amiloride band centered and lambda ex = 362 nm and lambda em = 415 nm. As a result, the determination was performed in an ethanol-water (1 + 1, v/v) medium at pH 6.3, adjusted by using sodium citrate-citric acid (0.1 M) as buffer solution. The concentration of amiloride in urine samples can be calculated by recording its total luminescence spectrum and applying the isopotential trajectory of the urine that cuts the selected band of amiloride. The unknown analytical signal of urine is eliminated in the MISF spectrum obtained, be means of its first derivative. A calibration graph was constructed by measuring first derivative values at lambda ex = 357nm and lambda em = 392 nm. Analytical parameters of the proposed method were calculated according to the error propagation theory. The sensitivity, repeatability, reproducibility and limit of determination achieved with the proposed method are adequate for the determination of amiloride in urine.

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Section: Methodsmentioning

confidence: 99%

Section: Determination Of Amiloride In Urinementioning

confidence: 96%

Section: Softwarementioning

confidence: 99%

mentioning

confidence: 99%

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Direct Determination of Amiloride in Urine Using Isopotential Fluorimetry

Pulgarı́n

Molina²,

López³

1997

Analyst

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“…To get the most sensitivity this trajectory should pass through the fluorescence maximum of both analytes. This trajectory is obtained by means of BASIC program developed by our research group [12]. This technique has been used for drug determinations in complex matrix like biological fluids and can be improved by the application of derivative methods and the zero-crossing technique [13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of two anti-inflammatory drugs in serum using isopotential fluorimetry

Pulgarı́n

Molina

Robles

2008

Analytica Chimica Acta

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Antibiotics, Pharmaceutical Analysis of

Gilpin

Pachla³

2000

Encyclopedia of Analytical Chemistry

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An antibiotic is a chemical substance, produced by microorganisms (or recently, synthetic routes) which has the capacity to inhibit the growth of other microorganisms/bacterial agents. The terminology for antibiotics stems from the Latin term of antibiosis and the noun antibiotic was coined by Professor Waksman in 1942. Considered as a single therapeutic class, antibiotics are one of the most diverse groups of medicinal agents. They can be grouped into several subclasses depending on their source, structure and activity. These are the cephalosporins, penicillins, quinolones, streptomyces and tetracycline. In addition to these agents, the sulfonamides and other miscellaneous compounds are also included owing to their antibacterial activity.

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New Rapid Assay for Nafcillin by Spectrofluorimetry in Pharmaceutical Dosage

Cited by 8 publications

References 10 publications

Direct Determination of Amiloride in Urine Using Isopotential Fluorimetry

Direct Determination of Amiloride in Urine Using Isopotential Fluorimetry

Simultaneous determination of two anti-inflammatory drugs in serum using isopotential fluorimetry

Antibiotics, Pharmaceutical Analysis of

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