New procedure for separation and analysis of the main components of cyanobacterial EPS

Copyrolysis of Biomass with Ca3(PO4)2 and MgO for Loading Enterobacter hormaechei Wu15 to Promote Pea Growth in Saline–Alkali Soil

“…56,57 Moreover, EPS contains extracellular DNA (eDNA), proteins, polysaccharides, and divalent cations. 58,59 Polysaccharides can promote the attachment of nitrogen fixing bacteria to plant roots and soil particles, thereby mediating a symbiotic relationship, which is equally important for root and rhizosphere colonization. The presence of divalent cations also facilitates the absorption of nutrients by plant roots.…”

“…Hence, Ca 3 (PO 4 ) 2 and MgO play a very positive role in the adsorption performance of biochar, and the Wu15 adsorption of BC1Ca8Mg2 increases by 11.1% compared with that of BC (Figure ). Second, PGPR is beneficial for plant growth in saline–alkali soils, mainly through effective rhizosphere colonization to exert biological control functions. , Previous studies have shown that PGPR “responds” to external environmental signals and forms a biofilm, which is composed of a group of cells that aggregate or adhere to the surface. , These cells are embedded in their own extracellular polymer (EPS) matrix, which can provide a protective barrier for plants against harsh environments and salt stress. , Moreover, EPS contains extracellular DNA (eDNA), proteins, polysaccharides, and divalent cations. , Polysaccharides can promote the attachment of nitrogen fixing bacteria to plant roots and soil particles, thereby mediating a symbiotic relationship, which is equally important for root and rhizosphere colonization. The presence of divalent cations also facilitates the absorption of nutrients by plant roots.…”

Deciphering the Roles of Extracellular Polymeric Substances (EPS) in Shaping Disinfection Kinetics through Permanent Removal via Genetic Disruption

“…To examine the feasibility of the genetic disruption strategy for validating the lasting absence of EPS without affecting cell viability, we compared it with the two conventional EPS removal methods, shaking and ultrasonication. ,, Although TEM images of the cells immediately after the treatments showed that both conventional methods could disrupt the EPS matrix surrounding the microbial cells, they failed to eliminate the EPS completely (Figure A) and exhibited denser cellular interactions (Figure S4). By contrast, the genetic disruption resulted in the complete removal of the EPS capsule layer from cells.…”

“…The intricate compositions and varied structures of EPS make their isolation from microbial cellular surfaces to create an enduringly EPS-deficient model strain, while minimizing the impact on microbial cells, a complex and difficult task. Several physical and chemical methods, including centrifugation, sonication, NaOH, cation exchange resin, or a combination of these methods, have been applied. − However, these methods have shown limited effectiveness, often leading to cell lysis . Additionally, the EPS complex can reassemble and regrow, impeding in situ and time-continuous studies.…”

Nachhaltigkeit in der Bioverfahrenstechnik

“…Die Pigmente und Phycobiliproteine ko ¨nnen im Anschluss chromatographisch aufgetrennt, charakterisiert und be-stimmt, oder die Konzentration mittels UV-VIS berechnet werden. Zur Charakterisierung der EPS wurde eine Separationsmethode entwickelt, die es ermo ¨glicht, die EPS in ihre drei Hauptbestandteile aufzuteilen -(i) Polysaccharide, (ii) Proteine und (iii) Fettsa ¨uren -sowie im Anschluss daran mittels chromatographischer oder kolorimetrischen Methoden zu analysieren [34,35]. Alternativ ko ¨nnen die EPS, oder einzelne Fraktionen auch auf ihre antimikrobielle Wirkung hin untersucht werden [14].…”