New polymorphic microsatellite markers of the endangered meadow viper (Vipera ursinii) identified by 454 high-throughput sequencing: when innovation meets conservation

Metzger, César; Ferchaud, Anne‐Laure; Geiser, Céline; Ursenbacher, Sylvain

doi:10.1007/s12686-011-9411-x

Cited by 10 publications

(8 citation statements)

References 15 publications

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“…The use of NGS in this study and numerous others (e.g. Castoe et al, 2010;Metzger et al, 2011;Frère et al, 2012) demonstrated that this methodology constitutes an efficient, rapid and economic way to characterise numerous polymorphic genetic markers.…”

supporting

confidence: 64%

“…However, recent high-throughput genomic sequencing technologies (e.g. 454 pyrosequencing) in combination with bioinformatics tools now represent a fast and cost-effective method to produce a large amount of multi-locus species-specific markers (Abdelkrim et al, 2009;Santana et al, 2009;Metzger et al, 2011). In this study, we used this method to develop specific microsatellite loci markers for the Asp viper and test for polymorphism within a Swiss population.…”

mentioning

confidence: 99%

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Development of polymorphic microsatellite loci markers for the Asp viper (Vipera aspis) using high-throughput sequencing and their use for other European vipers

et al. 2013

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The Asp viper (Vipera aspis) is a highly endangered species in the Swiss Plateau and in the Jura Mountains of Switzerland. In the past, numerous populations disappeared due to human activities, but currently the trend is reversed and several locations seem favourable for this species. However, it is not known if the remaining populations are still genetically variable and viable. In this study, 12 new mierosatellite loci markers were developed for Vipera aspis using Next Generation Sequencing (NGS) techniques (454 sequencing). All markers proved to be polymorphic with 2-9 alíeles. When cross-tested with two Eurasian widespread viper species, 8 of the newly developed loci provided reliable amplification for V. berus and 3 loci for V. ammodytes.

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confidence: 64%

mentioning

confidence: 99%

Development of polymorphic microsatellite loci markers for the Asp viper (Vipera aspis) using high-throughput sequencing and their use for other European vipers

et al. 2013

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“…In preliminary studies we tested published microsatellite primers that had been developed for adders (V. berus) [43,44], selecting five polymorphic loci that we found to amplify consistently, with a minimum of stutter bands. To increase the number of loci, we also evaluated 15 congeneric microsatellite markers which had been developed for meadow vipers (V. ursinii) [45], selecting three that successfully amplified and demonstrated polymorphism in adder samples (results not shown). PCR was performed in 10 μl volumes with 20-100 ng DNA, 5 μl mastermix (HotStarTaq Plus or Multiplex; Qiagen), 5 μmol/L unlabelled reverse primer and 5 μmol/L fluorophore-labelled forward primer (Applied Biosystems).…”

Section: Microsatellite Genotypingmentioning

confidence: 99%

Genetic and demographic vulnerability of adder populations: Results of a genetic study in mainland Britain

Ball

Hand²,

Willman

et al. 2020

PLoS ONE

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Genetic factors are often overlooked in conservation planning, despite their importance in small isolated populations. We used mitochondrial and microsatellite markers to investigate population genetics of the adder (Vipera berus) in southern Britain, where numbers are declining. We found no evidence for loss of heterozygosity in any of the populations studied. Genetic diversity was comparable across sites, in line with published levels for mainland Europe. However, further analysis revealed a striking level of relatedness. Genetic networks constructed from inferred first degree relationships suggested a high proportion of individuals to be related at a level equivalent to that of half-siblings, with rare inferred full-sib dyads. These patterns of relatedness can be attributed to the high philopatry and low vagility of adders, which creates high local relatedness, in combination with the polyandrous breeding system in the adder, which may offset the risk of inbreeding in closed populations. We suggest that reliance on standard genetic indicators of inbreeding and diversity may underestimate demographic and genetic factors that make adder populations vulnerable to extirpation. We stress the importance of an integrated genetic and demographic approach in the conservation of adders, and other taxa of similar ecology.

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“…Eight microsatellite markers (17FAM, 37NED, 3NED, 64VIC, 71FAM from Carlsson et al ., ; and Vu55FAM, Vu38VIC, Vu58VIC from Metzger et al . ) were used to identify the genetic background of each individual. These markers can also be used in paternity analysis, when questions arise about the origin of certain individuals, and for the selection of genetic sires from the available options in a breeding group.…”

Section: Hungarian Meadow Viper Conservation Centrementioning

confidence: 99%

Conservation of the Hungarian meadow viper Vipera ursinii rakosiensis

Péchy¹,

Halpern²,

Sós³

et al. 2014

International Zoo Yearbook

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In order to stop the decline of Hungarian meadow viper Vipera ursinii rakosiensis, in 2004 MME BirdLife Hungary together with national parks and Budapest Zoo started a complex conservation programme, supported by the European Union LIFE-Nature fund. The Hungarian Meadow Viper Conservation Centre was established with 16 adult individuals, collected from six different populations. By 2013 the number of vipers bred reached c. 1700 individuals. First reintroductions took place in March 2010, with 30 adult snakes released into a reconstructed habitat in Kiskunság National Park. By 2013, a total of 240 snakes had been released into three locations. Snakes were released by relocating the animals in the artificial burrows they used in the semi-natural terrariums at the Hungarian Meadow Viper Conservation Centre. At the release sites vipers were recorded 255 times during post-release monitoring, and 69 individuals were identified. Eighteen of the observed ΘΘ were gravid, and ten juvenile or subadult individuals were documented. In order to develop a remote-tracking method, pre-programmed radio-tags with a detection range of 200-300 m were surgically implanted into the abdomens of 16 vipers. These tags also operated as temperature loggers, recording data every 5 minutes for one year. Zoos play an important role in communicating the results of this captive-breeding and release programme. Exhibits of live Hungarian meadow vipers are located at Budapest Zoo and Schönbrunn Zoo in Vienna, Austria, and there are information points about the species located in all Hungarian zoos.

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New polymorphic microsatellite markers of the endangered meadow viper (Vipera ursinii) identified by 454 high-throughput sequencing: when innovation meets conservation

Cited by 10 publications

References 15 publications

Development of polymorphic microsatellite loci markers for the Asp viper (Vipera aspis) using high-throughput sequencing and their use for other European vipers

Development of polymorphic microsatellite loci markers for the Asp viper (Vipera aspis) using high-throughput sequencing and their use for other European vipers

Genetic and demographic vulnerability of adder populations: Results of a genetic study in mainland Britain

Conservation of the Hungarian meadow viper Vipera ursinii rakosiensis

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