New poly(ionic liquid)-grafted silica multi-mode stationary phase for anion-exchange/reversed-phase/hydrophilic interaction liquid chromatography

Abstract

A new poly(ionic liquid)-grafted silica stationary phase was prepared and characterized. It was then applied for multi-mode chromatographies, including ion-exchange, reversed-phase, and hydrophilic interaction liquid chromatographies for the effective separation of anions, hydrophobic compounds, and small polar molecules, respectively.

Glucaminium ionic liquid-functionalized stationary phase for the separation of nucleosides in hydrophilic interaction chromatography

“…The high conversion rate of tertiary amine was visualized by the exiguous difference between calculated and experimental C/N ratio. From the value of nitrogen (N) content (average of the two tests), the density of the glucaminium bonded to the surface of silica was calculated to be 0.64 μmol m −2 , according to the following equation [27][28][29][30]:…”

Versatile ligands for high-performance liquid chromatography: An overview of ionic liquid-functionalized stationary phases

“…Polymeric SCIL SP could be prepared not only from imidazoles with alkenyl as substituent, but also from imidazole with substituent containing alkenyl group [89]. An v-bromoester synthesized from acryloyl chloride and 11-bromoundecanol was employed to quaternize 1-methylimidazole, the obtained polymerizable IL possessed two polar sites.…”

Novel imidazolium-embedded N,N-dimethylaminopropyl-functionalized silica-based stationary phase for hydrophilic interaction/reversed-phase mixed-mode chromatography

“…Since it could produce multiple interaction mechanisms between the stationary phases and analytes, complex samples with different characteristics could be efficiently separated via mixed-mode stationary phases. Recently, a variety of mixedmode stationary phases, including RPLC/anion-exchange chromatography [10][11][12][13][14], RPLC/cation-exchange chromatography [15], HILIC/anion-exchange chromatography [16][17][18], HILIC/cation-exchange chromatography [19], RPLC/NPLC/ anion-exchange chromatography [20], HILIC/RPLC/anionexchange chromatography [21,22], and HILIC/RPLC [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39], have been reported and further used for separation of both small molecules and macromolecules. For example, Sun et al [12] prepared benzimidazole modified silica-based stationary phase for RPLC/anion-exchange chromatography.…”

“…Recently, they have been exploited for preparation of novel stationary phases [11,21,30]. For example, in our previous work, imidazoliumembedded amine stationary phase was designed and prepared.…”