New plant binary vectors with selectable markers located proximal to the left T-DNA border

Becker, Detlef; Kemper, Elke; Schell, Jeff; Masterson, Robert

doi:10.1007/bf00028908

Cited by 565 publications

(406 citation statements)

References 11 publications

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“…The lengths of the amplified sequences were 1867 bp (AtCKX1), 1603 bp (AtCKX2), 1452 bp (AtCKX3), 1926 bp (AtCKX4), 1817 bp (AtCKX5), and 1828 bp (AtCKX6). All promoter sequences were inserted into vector pGPTV-BAR (Becker et al, 1992) upstream of the GUS reading frame. Dideoxy sequencing of the amplified DNA fragments was performed with an ABI PRISM BigDye Terminator cycle sequencing reaction kit (Perkin-Elmer Applied Biosystems Division) to exclude PCR and cloning errors.…”

Section: Generation Of Atckx-overexpressing Plants and Transgenic Plamentioning

confidence: 99%

Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity

Werner

Motyka

Laucou³

et al. 2003

Plant Cell

1,272

1,243

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Cytokinins are hormones that regulate cell division and development. As a result of a lack of specific mutants and biochemical tools, it has not been possible to study the consequences of cytokinin deficiency. Cytokinin-deficient plants are expected to yield information about processes in which cytokinins are limiting and that, therefore, they might regulate. We have engineered transgenic Arabidopsis plants that overexpress individually six different members of the cytokinin oxidase/dehydrogenase ( AtCKX ) gene family and have undertaken a detailed phenotypic analysis. Transgenic plants had increased cytokinin breakdown (30 to 45% of wild-type cytokinin content) and reduced expression of the cytokinin reporter gene ARR5 : GUS ( ␤ -glucuronidase). Cytokinin deficiency resulted in diminished activity of the vegetative and floral shoot apical meristems and leaf primordia, indicating an absolute requirement for the hormone. By contrast, cytokinins are negative regulators of root growth and lateral root formation. We show that the increased growth of the primary root is linked to an enhanced meristematic cell number, suggesting that cytokinins control the exit of cells from the root meristem. Different AtCKX-green fluorescent protein fusion proteins were localized to the vacuoles or the endoplasmic reticulum and possibly to the extracellular space, indicating that subcellular compartmentation plays an important role in cytokinin biology. Analyses of promoter: GUS fusion genes showed differential expression of AtCKX genes during plant development, the activity being confined predominantly to zones of active growth. Our results are consistent with the hypothesis that cytokinins have central, but opposite, regulatory functions in root and shoot meristems and indicate that a fine-tuned control of catabolism plays an important role in ensuring the proper regulation of cytokinin functions.

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Section: Generation Of Atckx-overexpressing Plants and Transgenic Plamentioning

confidence: 99%

Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity

Werner

Motyka

Laucou³

et al. 2003

Plant Cell

1,272

1,243

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“…The pGPTVkan-LBC3, kindly provided by Mette Groenlund (University of Aahrus, Denmark), is a derivative of the pGPTVkan plasmid (Becker et al 1992), containing the 1.9 kb fragment of the Glycine max LBC3 promoter cloned into SalI-BamHI sites.…”

Section: T-dna Constructs Preparationmentioning

confidence: 99%

Tissue-specific down-regulation of LjAMT1;1 compromises nodule function and enhances nodulation in Lotus japonicus

et al. 2008

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Plant ammonium transporters of the AMT1 family are involved in N-uptake from the soil and ammonium transport, and recycling within the plant. Although AMT1 genes are known to be expressed in nitrogen-fixing nodules of legumes, their precise roles in this specialized organ remain unknown. We have taken a reverse-genetic approach to decipher the physiological role of LjAMT1;1 in Lotus japonicus nodules. LjAMT1;1 is normally expressed in both the infected zone and the vascular tissue of Lotus nodules. Inhibition of LjAMT1;1 gene expression, using an antisense gene construct driven by a leghemoglobin promoter resulted in a substantial reduction of LjAMT1;1 transcript in the infected tissue but not the vascular bundles of transgenic plants. As a result, the nitrogen-fixing activity of nodules was partially impaired and nodule number increased compared to control plants. Expression of LjAMT1;1-GFP fusion protein in plant cells indicated a plasma-membrane location for the LjAMT1;1 protein.Taken together, the results are consistent with a role of LjAMT1;1 in retaining ammonium derived from symbiotic nitrogen fixation in plant cells prior to its assimilation.

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“…Two series of binary vectors, pB and pH types, carrying a phosphinothricin N-acetyltransferase (BAR) or a hygromycin phosphotransferase gene (HPT), respectively, were similarly constructed from pGPTV-BAR or pGPTV-HYG (Becker et al, 1992) as follows.…”

Section: Construction Of Binary Vectors and Transformation Of Plantsmentioning

confidence: 99%

Functional conservation and maintenance of expression pattern of FIDDLEHEAD-like genes in Arabidopsis and Antirrhinum

Efremova¹,

Schreiber

Bär³

et al. 2004

Plant Mol Biol

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In Arabidopsis, loss of function of the epidermis-specific FDH gene coding for a putative b-ketoacyl-CoA synthase results in ectopic organ fusions in mutants. Corresponding mutants are not available for Antirrhinum majus, however, organ fusions can be induced in both species by chloroacetamide inhibitors of b-ketoacyl-CoA synthases using a chemical genetics approach. We isolated the ortholog of FDH from Antirrhinum majus, the ANTIRRHINUM FIDDLEHEAD (AFI ) gene, and showed that AFI complements fdh when expressed in the epidermis under control of the FDH promoter. Like FDH, the AFI gene exhibits protodermis-and epidermis-specific expression, and its promoter directs the expression of reporter genes to the epidermis in transgenic Antirrhinum and Arabidopsis. We demonstrate down-regulation of the FDH promoter in the epidermis of the ovary septum, thereby supporting the assumption that FDH-like genes may directly facilitate the cell-cell interactions that need to occur during carpel fusion and pollen tube growth. Up-regulation of FDH in the stomium, on the other hand, provides evidence for its possible involvement in cell separation during anther dehiscence. Down-regulation of the FDH and AFI promoters in the septum is observed in transgenic Arabidopsis but not in Antirrhinum plants. This probably reflects differences in the ontogeny of the ovary septum between the two species. We also show that epidermis-specific FDH-like genes may not be able to efficiently elongate fatty acid chains when misexpressed in seeds.

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New plant binary vectors with selectable markers located proximal to the left T-DNA border

Cited by 565 publications

References 11 publications

Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity

Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity

Tissue-specific down-regulation of LjAMT1;1 compromises nodule function and enhances nodulation in Lotus japonicus

Functional conservation and maintenance of expression pattern of FIDDLEHEAD-like genes in Arabidopsis and Antirrhinum

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