New pieces in the transmission cycle of the hepatitis E virus in South America: first viral detection in wild boars from Argentina

Pisano, María Belén; Winter, Marina; Raimondo, Natalia; Martinez‐Wassaf, Maribel G.; Abate, S; Ré, Viviana Elizabeth

doi:10.1093/trstmh/trz034

Cited by 9 publications

(7 citation statements)

References 7 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These researchers also report finding the largest proportion of HEV viremic pigs that excrete viruses via faeces among the seropositive and younger animals. Results corroborating seroconversion in artificially or naturally HEV infected pigs have been presented by several researchers, without statements on statistical calculations, however [49][50][51]. Furthermore, comparative studies on the detection of HEV RNA in the liver, which represents the target organ for HEV, and in serum positively screened wild boars showed almost identical results [18,52].…”

Section: Discussionmentioning

confidence: 84%

Detection of Hepatitis E Virus Infections in Wild Boars in Southwest Germany Using a Stepwise Laboratory Diagnostic Approach

et al. 2022

View full text Add to dashboard Cite

Hepatitis E virus (HEV) is the main cause of enterically transmitted hepatitis in humans worldwide. Among HEV, genotypes 3 and 4 are considered zoonotic agents associated with domestic pigs and wild boars, showing an increasing trend in Europe. The aim of this study is to contribute data on the prevalence of HEV in wild boars in Southwest Germany and to present a time and cost-effective two-step laboratory diagnostic approach for serological monitoring of blood samples. This method uses enzyme-linked immunosorbent assay (ELISA), followed by testing for HEV RNA by reverse transcription real-time PCR (RT-qPCR). A total of 2295 blood samples were collected in 234 municipalities in 12 counties in the period from 2016 to 2020. There was an overall seroprevalence of 10.8%, ranging from 3.6% to 17.5% per county and 7.5% to 14% per year. Retesting of these blood samples for HEV RNA revealed 15.7% viremic wild boars originating from 30 municipalities in 11 counties. Viremic wild boars were found in seven regional clusters, including 84% of the animals that tested positive for HEV. Seropositive animals <1 year of age were significantly more likely to be viremic than those >1 year. Further characterization of HEV RNA resulted in the identification of genotype 3. Altogether, serological monitoring of the blood samples, complemented by successive and targeted investigations into the presence of HEV RNA based on blood samples, provide reliable information on the seroprevalence and virus load in wild boars, which proved to be a relevant and persistent sylvatic reservoir for HEV.

show abstract

Section: Discussionmentioning

confidence: 84%

Detection of Hepatitis E Virus Infections in Wild Boars in Southwest Germany Using a Stepwise Laboratory Diagnostic Approach

et al. 2022

View full text Add to dashboard Cite

show abstract

“…ELISA testing for HEV was performed in 101 serum samples from captive adult peccaries held in two reserves. Of note, the ELISA kit used in this study has been extensively used for the detection of anti‐HEV antibodies in human and non‐human reservoirs (Mirazo et al, 2018; Pankovics et al., 2020; Pisano et al., 2019). Specific anti‐HEV antibodies were found in animals from both enclosures with an overall prevalence of 24.7% (25/101).…”

Section: Resultsmentioning

confidence: 99%

Hepatitis E Virus (HEV) infection in captive white‐collared peccaries ( Pecari tajacu ) from Uruguay

Ferreiro

Herrera

Gonzalez

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

Hepatitis E virus (HEV) is a major cause of acute hepatitis worldwide with 3.3 million symptomatic cases per year and about 70.000 deaths (World Health Organization, 2017). In high-income countries and non-endemic regions, Hepatitis E is regarded as an emerging disease of increasing concern. In this setting, cases are mainly sporadic and autochthonous, and infection seems to occur zoonotically by direct contact or through the consumption of raw or undercooked meat from reservoir animals (Colson & Decoster, 2019). HEV belongs

show abstract

“…In contrast, immunoassays such as ELISA, western blot, and immuno uorescence, can be used to detect viral particles and antibodies, their set-up is simpler, and the cost is lower [43][44][45][46] . Some of the serological diagnostic tests for humans have been adapted to detect antibodies in animals 33,34 . A disadvantage of these methods is that they require conventional antibodies (polyclonal or monoclonal antibodies) as their main reagents and their production is complex and expensive.…”

Section: Discussionmentioning

confidence: 99%

“…In the U.S. there is a commercial kit available for swine based on the ORF2 and ORF3 proteins (PrioCHECK® HEV Antibody ELISA) 32 . Researchers have used kits intended for humans and changed the speci c conjugated antibody for swine 33 . Other detection kits are based on a sandwich ELISA and detect anti-HEV antibodies in different species using ORF2 or a synthetic peptide as both capture and detection reagent, the latter conjugated with Horse Radish Peroxidase (HRP) 34 .…”

Section: Introductionmentioning

confidence: 99%

A Multispecies Competitive Nanobody-Based Elisa for the Detection of Antibodies Against Hepatitis E Virus

Arce

Pavan

Bok

et al. 2023

Preprint

View full text Add to dashboard Cite

The hepatitis E virus (HEV) is an emergent zoonotic virus causing viral hepatitis worldwide. Clinically, hepatitis E is not easily distinguished from other types of acute viral hepatitis. There is a need for HEV diagnostic assays to detect and prevent interspecies transmission among susceptible populations. Nanobodies (Nbs) are expressed recombinantly in different systems, produced with high yields, and have superior physicochemical properties compared with conventional antibodies (Ab). Several Nbs against ORF2, the capsid protein and main antigen, were selected and produced in E. coli. Nb39 and Nb74 specifically recognized HEV ORF2 (genotypes 3 and 4). A competitive ELISA (cELISA) was developed and validated using a reference panel of human (n = 86) and swine sera (n = 116) tested in comparison with a commercial kit. The optimal cutoff values determined by ROC analysis were 69.16% (human) and 58.76% (swine); the sensitivity and specificity were high: 97.4% (IC95%; 86.5–99.5%) and 95.8% (IC95%; 86.0-98.8%) for human vs. 100% (IC95%: 93.5–100%) and 98.3% (IC95%: 91.0-99.7%) for swine. Further, the cELISA detected total anti-HEV antibodies in wild boar, deer, and mice. To our knowledge, this is the first report of production of Nbs against HEV-3 ORF2 for diagnostic purposes.

show abstract

New pieces in the transmission cycle of the hepatitis E virus in South America: first viral detection in wild boars from Argentina

Cited by 9 publications

References 7 publications

Detection of Hepatitis E Virus Infections in Wild Boars in Southwest Germany Using a Stepwise Laboratory Diagnostic Approach

Detection of Hepatitis E Virus Infections in Wild Boars in Southwest Germany Using a Stepwise Laboratory Diagnostic Approach

Hepatitis E Virus (HEV) infection in captive white‐collared peccaries ( Pecari tajacu ) from Uruguay

A Multispecies Competitive Nanobody-Based Elisa for the Detection of Antibodies Against Hepatitis E Virus

Contact Info

Product

Resources

About