New Phenolic Compounds in Posidonia oceanica Seagrass: A Comprehensive Array Using High Resolution Mass Spectrometry

Abstract

The studies on the Posidonia oceanica Delile (P. oceanica) phenolic composition have been focused on the foliar tissues and have often neglected the phenolic compounds in rhizomes or roots alike. With the current improvements in high resolution mass spectrometry (HRMS) analyzers, such as the Orbitrap MS, there is a new opportunity to more deeply study P. oceanica. One of the benefits is the possibility of conducting an exhaustive phenolic monitoring, which is crucial in the search for new stressor-specific biomarkers of coastal deterioration. For this purpose, the different tissues (leaf, rhizome, and root) of P. oceanica seagrass from several marine sampling areas were analyzed through target, suspected, and non-target screenings. This paper brings a fast and tissues-specific extraction, as well as a detection method of phenolic compounds applying for the first time the potential of HRMS (Exactive Orbitrap) in P. oceanica samples. As a result, 42 phenolic compounds were satisfactorily detected, of which, to our knowledge, 24 were not previously reported in P. oceanica, such as naringenin, naringenin chalcone and pinocembrin, among others. Information here reported could be used for the evaluation of new stressor-specific biomarkers of coastal deterioration in the Mediterranean waters. Furthermore, the followed extraction and analytical method could be considered as a reference protocol in other studies on marine seagrasses due to the exhaustive search and satisfactory results.

Antioxidant Bioactivity of Extracts from Beach Cast Leaves of Posidonia oceanica (L.) Delile

“…P. oceanica leaves have a high content of secondary metabolites, mainly represented by phenolic compounds making this seagrass a potential useful source of antioxidant molecules, to counter oxidative stress, which leads wrinkle formation, skin aging, cancer and cardiovascular diseases [1,7,[30][31][32][33]. Excessive and prolonged exposure to ultra-violet radiation (UV) is one of the main factor for the cellular over-production of radical species, such as reactive oxygen species (ROS) [34,35].…”

Grain Germination Changes the Profile of Phenolic Compounds and Benzoxazinoids in Wheat: A Study on Hard and Soft Cultivars

“…The phytochemical compounds of wheat extracts were identified using two different sets of liquid chromatograph (LC) equipment. The first exploratory analysis was conducted using an LC system connected to a mass spectrometer (MS) that had a hybrid Orbitrap analyzer and an electrospray ionization source (ESI, Q-ExactiveTM, Thermo Fisher Scientific, Bremen, Germany), using the chromatographic conditions previously described by Astudillo-Pascual et al [ 62 ].…”

Determination of PAHs, PAH-Derivatives and Other Concerning Substances in Posidonia oceanica Seagrass and Marine Sediments by High Resolution Mass Spectrometry

“…Following a procedure already described in Astudillo-Pascual et al [35], P. oceanica seagrasses were cleaned from sand and salt using distilled water. Afterward, adult leaves and rhizomes were selected for further procedures, while roots, young leaves, basal sheath, and epiphytes were removed and not considered in this study.…”