New perspectives on an ancient pathogen: thoughts for World Tuberculosis Day 2022

Beste, Dany J. V.

doi:10.1099/mic.0.001178

Cited by 2 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While the cell envelope collection is still publishing more articles, many of which I will cover in the Summer Musings , the Mycobacterial collection, led by Microbiology Senior Editor Riccardo Manganelli, now contains 28 new and retrospective pieces. Mycobacterium tuberculosis (Mtb) expert and Microbiology Editor, Dany Beste (@danybeste), from the University of Surrey, UK, closes out the collection with a reflective piece on this ancient pathogen [16] and pulls together the diverse articles included in the collection. She focusses on papers revealing fundamental mechanisms of cell growth and survival, as well as others relating to targets for the development of new antimicrobials, including improved understanding of its particularly complex cell envelope.…”

Section: Full-textmentioning

confidence: 99%

Microbial Musings – Spring 2022

Thomas

2022

Microbiology

View full text Add to dashboard Cite

Section: Full-textmentioning

confidence: 99%

Microbial Musings – Spring 2022

Thomas

2022

Microbiology

View full text Add to dashboard Cite

Expanding the boundaries of atomic spectroscopy at the single-cell level: critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells

et al. 2023

View full text Add to dashboard Cite

Metals have a fundamental role in microbiology, and accurate methods are needed for their identification and quantification. The inability to assess cellular heterogeneity is considered an impediment to the successful treatment of different diseases. Unlike bulk approaches, single-cell analysis allows elemental heterogeneity across genetically identical populations to be related to specific biological events and to the effectiveness of drugs. Single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS) can analyse single cells in suspension and measure this heterogeneity. Here we explore advances in instrumental design, compare mass analysers and discuss key parameters requiring optimisation. This review has identified that the effect of pre-treatment of cell suspensions and cell fixation approaches require further study and novel validation methods are needed as using bulk measurements is unsatisfactory. SP-ICP-MS has the advantage that a large number of cells can be analysed; however, it does not provide spatial information. Techniques based on laser ablation (LA) enable elemental mapping at the single-cell level, such as laser-induced breakdown spectroscopy (LIBS) and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The sensitivity of commercial LIBS instruments restricts its use for sub-tissue applications; however, the capacity to analyse endogenous bulk components paired with developments in nano-LIBS technology shows great potential for cellular research. LA-ICP-MS offers high sensitivity for the direct analysis of single cells, but standardisation requires further development. The hyphenation of these trace elemental analysis techniques and their coupling with multi-omic technologies for single-cell analysis have enormous potential in answering fundamental biological questions.

show abstract

New perspectives on an ancient pathogen: thoughts for World Tuberculosis Day 2022

Cited by 2 publications

References 26 publications

Microbial Musings – Spring 2022

Microbial Musings – Spring 2022

Expanding the boundaries of atomic spectroscopy at the single-cell level: critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells

Contact Info

Product

Resources

About