New Perspectives in Stem Cell Transplantation and Associated Therapies to Treat Retinal Diseases: From Gene Editing to 3D Bioprinting

Bovi dos Santos, Gabrieli; de Lima-Vasconcellos, Théo Henrique; Móvio, Marília Inês; Birbrair, Alexander; Del Debbio, Carolina Beltrame; Kihara, Alexandre Hiroaki

doi:10.1007/s12015-024-10689-4

Cited by 3 publications

(1 citation statement)

References 158 publications

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“…Advances in gene therapy and retinal imaging have driven remarkable progress in the design of human clinical trials and clinical testing of novel therapeutics for IRDs [6][7][8][9]. This includes the utilization of small molecules, DNA-and RNA-based therapies, microelectrode arrays, and cellular transplantation [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Retinal Imaging Findings in Inherited Retinal Diseases

Corradetti,

Verma,

Tojjar

et al. 2024

JCM

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Inherited retinal diseases (IRDs) represent one of the major causes of progressive and irreversible vision loss in the working-age population. Over the last few decades, advances in retinal imaging have allowed for an improvement in the phenotypic characterization of this group of diseases and have facilitated phenotype-to-genotype correlation studies. As a result, the number of clinical trials targeting IRDs has steadily increased, and commensurate to this, the need for novel reproducible outcome measures and endpoints has grown. This review aims to summarize and describe the clinical presentation, characteristic imaging findings, and imaging endpoint measures that are being used in clinical research on IRDs. For the purpose of this review, IRDs have been divided into four categories: (1) panretinal pigmentary retinopathies affecting rods or cones; (2) macular dystrophies; (3) stationary conditions; (4) hereditary vitreoretinopathies.

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Section: Introductionmentioning

confidence: 99%

Retinal Imaging Findings in Inherited Retinal Diseases

Corradetti,

Verma,

Tojjar

et al. 2024

JCM

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Advances in induced pluripotent stem cell‐derived cardiac myocytes: technological breakthroughs, key discoveries and new applications

Clancy,

Santana

2024

The Journal of Physiology

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A transformation is underway in precision and patient‐specific medicine. Rapid progress has been enabled by multiple new technologies including induced pluripotent stem cell‐derived cardiac myocytes (iPSC‐CMs). Here, we delve into these advancements and their future promise, focusing on the efficiency of reprogramming techniques, the fidelity of differentiation into the cardiac lineage, the functional characterization of the resulting cardiac myocytes, and the many applications of in silico models to understand general and patient‐specific mechanisms controlling excitation–contraction coupling in health and disease. Furthermore, we explore the current and potential applications of iPSC‐CMs in both research and clinical settings, underscoring the far‐reaching implications of this rapidly evolving field. image

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Bioprinting of a Biomimetic Microenvironment for a Retinal Regenerative Approach

Belgio,

Mantero,

Iervolino

et al. 2024

Applied Sciences

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There is an ongoing effort to advance methodologies for culturing functional photoreceptors in vitro for retinal regenerative strategies. To support the formation of functional photoreceptors, a scaffold should replicate the native environment. The aim of this study was to optimize a sodium alginate–gelatin (SA-G) bioink to mimic the retinal properties while ensuring the printing of constructs with high shape fidelity. The optimized bioink was thoroughly characterized in terms of its physical, mechanical, and rheological properties, printability assessment, and preliminary biocompatibility. The material showed a constant degradation rate, which is crucial for effective tissue regeneration as it provides support for cell differentiation and polarization while gradually degrading to allow cell proliferation and matrix deposition. The optimized bioink displayed stiffness comparable to the native photoreceptor layer, potentially providing appropriate mechanical cues for photoreceptor maturation. Additionally, it exhibited shear-thinning behavior, the presence of yield stress, and fast recovery kinetics, which are essential for successful extrusion. The high shape fidelity of 3D-printed constructs suggested the feasibility of printing complex patterns to drive photoreceptor polarization. The preliminary cell results demonstrated homogeneous cell distribution and sustained cell viability over time. Overall, these findings indicate that the optimized bioink can provide the mechanical and topographical cues necessary for cultivating photoreceptors in vitro for retinal regeneration.

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New Perspectives in Stem Cell Transplantation and Associated Therapies to Treat Retinal Diseases: From Gene Editing to 3D Bioprinting

Cited by 3 publications

References 158 publications

Retinal Imaging Findings in Inherited Retinal Diseases

Retinal Imaging Findings in Inherited Retinal Diseases

Advances in induced pluripotent stem cell‐derived cardiac myocytes: technological breakthroughs, key discoveries and new applications

Bioprinting of a Biomimetic Microenvironment for a Retinal Regenerative Approach

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