New Perspectives for in Vitro Risk Assessment of Multiwalled Carbon Nanotubes: Application of Coculture and Bioinformatics

Abstract

Nanotechnology is a rapidly expanding field with wide application for industrial and medical use; therefore, understanding the toxicity of engineered nanomaterials is critical for their commercialization. While short term in vivo studies have been performed to understand the toxicity profile of various nanomaterials, there is a current effort to shift toxicological testing from in vivo observational models to predictive and high-throughput in vitro models. However, conventional monoculture results of nanoparticle exposure are often disparate and not predictive of in vivo toxic effects. A coculture system of multiple cell types allows for cross-talk between cells and better mimics the in vivo environment. This review proposes that advanced coculture models, combined with integrated analysis of genome-wide in vivo and in vitro toxicogenomic data, will lead to the development of predictive multi-gene expression-based models to better determine toxicity profiles of and the potential human health risk of exposure to nanomaterials.

Small airway epithelial cells exposure to printer-emitted engineered nanoparticles induces cellular effects on human microvascular endothelial cells in an alveolar-capillary co-culture model

“…The use of the co-culture model which allows for the study of epithelial and endothelial cellular communication within the alveolar-capillary unit, offers an advantage over mono-culture cell models commonly used in nanotoxicology studies. It is worth nothing that a comparative study recently performed by our group showed that the in vitro alveolar-capillary co-culture model correlates with in vivo results more closely than a mono-culture model upon treatment with multi-walled carbon nanotubes (MWCNT) (Snyder-Talkington BN, 2012). …”

Recent progress in synthesis, properties and potential applications of SiC nanomaterials

“…De hecho, la mayoría de las propiedades típicas que los caracteriza (pequeño tamaño en al menos una dimensión entre 1-100 nm, su gran área superficial por peso y su reactividad) son precisamente las propiedades que los hacen tecnológica-mente tan valiosos y estas son probablemente las razones para sus efectos dañinos potenciales [65,66,67].…”

Analysis of Dose-Dependent Effect of Zinc Oxide Nanoparticles on the Oxidative Stress and Antioxidant Enzyme Activity in Adipocytes

“…Generation of cytotoxicity due to the interaction of metal oxide nanoparticles with the biological system is still unclear [9]. There are several studies that have reported cytotoxicity of ZnO nanoparticles in various cell lines.…”