New perfluorinated polycationic dimerizable detergents for the formulation of monomolecular DNA nanoparticles and their in vitro transfection efficiency

Fabio, Karine; Giorgio, Christophe Di; Vierling, Pierre

doi:10.1016/j.bbagen.2005.03.007

Cited by 21 publications

(28 citation statements)

References 28 publications

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“…The resulting lipoplexes have a mean diameter of 23 nm, a negative zeta potential and are stable in physiological conditions. Then, numerous other studies were published following this pioneering approach [18,19].…”

Section: Colloidal Stability and Size Of Lipoplexesmentioning

confidence: 98%

Physicochemical Parameters of Non-Viral Vectors that Govern Transfection Efficiency

Barteau¹,

Chèvre²,

Letrou-Bonneval³

et al. 2008

CGT

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Gene therapy is based on the vectorization of nucleic acids to target cells and their subsequent expression. Cationic lipids and polymers are the most widely used vectors for the delivery of DNA into cultured cells. Nowadays, numerous reagents made of these cationic molecules are commercially available and used by researchers from the academic and industrial field. By contrast their evaluations in preclinical programs have revealed that their use for in vivo applications will be more problematic than their massive use in vitro. This is mostly due to the physicochemical properties of cationic vectors/DNA complexes, which are the result of their mode of interaction. Indeed, these cationic vectors interact through electrostatic forces with negatively charged DNA. This results in the formation of highly organized positively charged supramolecular structures where DNA molecules are condensed. Association of DNA with cationic lipids under a micellar or liposomal form leads to lamellar organization with DNA molecules sandwiched between lipid bilayers. Although the lamellar phase is the common described structure, as evidenced by small-angle X-ray scattering and electron microscopy, some cationic lipid combined with a hexagonal forming lipid could also result with DNA in an inverted hexagonal structure. Despite a lot of effort, the precise mechanism of gene transfer with cationic vector is still ill-defined. Here, our objective was to overview the main relationships between the physico chemical properties of cationic lipid/DNA complexes and their transfection efficiency. An overview of a new class of vectors consisting of amphiphilic block copolymers designed for in vivo delivery is also presented and discussed.

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Section: Colloidal Stability and Size Of Lipoplexesmentioning

confidence: 98%

Physicochemical Parameters of Non-Viral Vectors that Govern Transfection Efficiency

Barteau¹,

Chèvre²,

Letrou-Bonneval³

et al. 2008

CGT

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“…This process is the basis of the wide application of surfactants in isolation and purification of membrane proteins [4][5][6], in DNA extraction [7,8] and as drug delivery vehicles [9][10][11][12][13][14], among others.…”

Section: Introductionmentioning

confidence: 99%

Mixed micelle formation between amino acid-based surfactants and phospholipids

Faustino

Calado

García‐Río

2011

Journal of Colloid and Interface Science

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“…Several groups have also investigated the use of disulfide bonds to develop monomolecular particles by the dimerization of a thiol-containing, cationic detergent, entrapping only one DNA molecule per lipoplex (Blessing et al 1998;Ouyang et al 2000;Dauty et al 2001;Fabio et al 2005). The strategy relies on the ability of the cationic detergent to condense DNA into discrete particles that can consist of a single nucleic acid molecule.…”

Section: Release Of Dna In Cytoplasm By Intracellular Disulfide Reducmentioning

confidence: 99%

Nature as a source of inspiration for cationic lipid synthesis

Labas

Beilvert

Barteau³

et al. 2009

Genetica

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Synthetic gene delivery systems represent an attractive alternative to viral vectors for DNA transfection. Cationic lipids are one of the most widely used non-viral vectors for the delivery of DNA into cultured cells and are easily synthesized, leading to a large variety of well-characterized molecules. This review discusses strategies for the design of efficient cationic lipids that overcome the critical barriers of in vitro transfection. A particular focus is placed on natural hydrophilic headgroups and lipophilic tails that have been used to synthesize biocompatible and non-toxic cationic lipids. We also present chemical features that have been investigated to enhance the transfection efficiency of cationic lipids by promoting the escape of lipoplexes from the endosomal compartment and DNA release from DNA-liposome complexes. Transfection efficiency studies using these strategies are likely to improve the understanding of the mechanism of cationic lipid-mediated gene delivery and to help the rational design of novel cationic lipids.

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New perfluorinated polycationic dimerizable detergents for the formulation of monomolecular DNA nanoparticles and their in vitro transfection efficiency

Cited by 21 publications

References 28 publications

Physicochemical Parameters of Non-Viral Vectors that Govern Transfection Efficiency

Physicochemical Parameters of Non-Viral Vectors that Govern Transfection Efficiency

Mixed micelle formation between amino acid-based surfactants and phospholipids

Nature as a source of inspiration for cationic lipid synthesis

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