New parameters in assessment of human donor corneal stroma

Borderie, Marie; Grieve, Kate; Irsch, Kristina; Ghoubay, Djida; Georgeon, Cristina; Sousa, Céline de; Laroche, L.; Borderie, Vincent

doi:10.1111/aos.13351

Cited by 16 publications

(18 citation statements)

References 37 publications

(53 reference statements)

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“…Regarding ophthalmic applications, FFOCT has been used for the characterization of various ex vivo tissues from the anterior and posterior segments of animal eyes [43]. It was shown to provide valuable assessment of human corneal grafts, which could potentially improve outcome of corneal transplantation [44][45][46]. Grieve and colleagues pioneered in vivo ocular FFOCT by imaging cellular features of the anterior segment of the rat eye [47].…”

Section: Introductionmentioning

confidence: 99%

In vivo high resolution human corneal imaging using full-field optical coherence tomography

Mazlin¹,

Xiao²,

Dalimier³

et al. 2018

Biomed. Opt. Express

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We present the first full-field optical coherence tomography (FFOCT) device capable of imaging of the human cornea. We obtained images of the epithelial structures, Bowman's layer, sub-basal nerve plexus (SNP), anterior and posterior stromal keratocytes, stromal nerves, Descemet's membrane and endothelial cells with visible nuclei. Images were acquired with a high lateral resolution of 1.7 µm and relatively large field-of-view of 1.26 mm x 1.26 mm - a combination, which, to the best of our knowledge, has not been possible with other human eye imaging methods. The latter together with a contactless operation, make FFOCT a promising candidate for becoming a new tool in ophthalmic diagnostics.

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Section: Introductionmentioning

confidence: 99%

In vivo high resolution human corneal imaging using full-field optical coherence tomography

Mazlin¹,

Xiao²,

Dalimier³

et al. 2018

Biomed. Opt. Express

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“…At the Quinze-Vingts National Eye Hospital (in collaboration with Prof. Vincent Borderie's anterior segment service), we have been investigating the device's potential for examining donor corneas before transplantation, as an addition and improvement upon current eye-banking procedures for graft quality control [4]. In fact, we have already demonstrated the capability of FF-OCT to provide structural information superior to histology and to identify more disease indicators as was possible with the combination of clinical OCT and confocal microscopy [5]. Further, the technique was able to identify new quantitative parameters in the assessment of human donor corneal stroma [4,5] (note that the transparency of the cornea largely relates directly to stromal structure [6,7]).…”

Section: Methods and Resultsmentioning

confidence: 99%

“…In fact, we have already demonstrated the capability of FF-OCT to provide structural information superior to histology and to identify more disease indicators as was possible with the combination of clinical OCT and confocal microscopy [5]. Further, the technique was able to identify new quantitative parameters in the assessment of human donor corneal stroma [4,5] (note that the transparency of the cornea largely relates directly to stromal structure [6,7]). Most notably, objective analysis of stromal light backscattering with FF-OCT (also in collaboration with Prof. Karsten Plamann from the Laboratoire d'Optique et Biosciences) has shown promise in the quantification of corneal transparency, as well as in differentiating abnormal from normal corneal tissues [8].…”

Section: Methods and Resultsmentioning

confidence: 99%

Towards In-Vivo Characterization and Deep Imaging of the Cornea and Beyond

Irsch¹

2018

Latin America Optics and Photonics Conference

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“…8,9 We used corneal reflectivity (or backscattering) on optical coherence tomography (OCT) scans to assess changes in transparency as previously reported. 10 Corneal backscattering was measured with IMAGEJ® software (National Institutes of Health (NIH), Madison, WI, USA) using scans centered on the corneal vertex, and mean intensity depth profiles were generated (i.e. corneal backscattering as a function of depth).…”

Section: Sd-octmentioning

confidence: 99%

Multimodal imaging of pre-Descemet corneal dystrophy

Alafaleq¹,

Georgeon²,

Grieve³

et al. 2019

European Journal of Ophthalmology

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Purpose: The aim of this study was to assess structural and histological changes associated with pre-Descemet corneal dystrophy with multimodal in vivo imaging. Methods: Retrospective case series including eight corneas from four unrelated male patients with pre-Descemet corneal dystrophy characterized by the presence of punctiform gray opacities located just anterior to the Descemet membrane at slit-lamp examination of both eyes. In vivo confocal microscopy images were obtained in the central, paracentral, and peripheral corneal zones from the superficial epithelial cell layer down to the corneal endothelium in both eyes. Spectral domain optical coherence tomography scans (central and limbal zones) and mapping of both corneas were acquired. Results: Diffuse small extracellular stromal deposits, presence of enlarged hyperreflective keratocytes in the posterior stroma with either hyperreflective or hyporeflective intracellular dots, and presence of activated keratocytes in the very anterior stroma were observed in all corneas with in vivo confocal microscopy. Spectral domain optical coherence tomography scans showed a hyperreflective line anterior to Descemet’s membrane running from limbus to limbus and associated with a second thinner hyperreflective line just beneath Bowman’s layer. Fine hyperreflective particles were observed in the posterior, mid, and anterior stroma on optical coherence tomography scans. Conclusion: The clinical presentation and structural anomalies found in isolated sporadic pre-Descemet corneal dystrophy are in favor of a degenerative process affecting corneal keratocytes with no epithelial or endothelial involvement. The maximum damage is found just anterior to the Descemet membrane resulting in pre-Descemet membrane location of stromal opacities. Multimodal imaging of cornea reveals that the disorder affects the whole stroma and it permits better understanding of pre-Descemet corneal dystrophy pathophysiology together with ascertained diagnosis.

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New parameters in assessment of human donor corneal stroma

Abstract: Full-field optical coherence microscopy (FFOCM) provides precise and reliable parameters for non-invasive assessment of human donor corneal stroma during storage, enabling detection of stromal disorders that could impair the results of keratoplasty.

Cited by 16 publications

References 37 publications

In vivo high resolution human corneal imaging using full-field optical coherence tomography

In vivo high resolution human corneal imaging using full-field optical coherence tomography

Towards In-Vivo Characterization and Deep Imaging of the Cornea and Beyond

Multimodal imaging of pre-Descemet corneal dystrophy

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