New or little-known Boreoheptagyia (Diptera, Chironomidae) in China inferred from morphology and DNA barcodes

Abstract

The male adult of Boreoheptagyia zhengi Lin & Liu, sp. nov. is described and illustrated based on material collected in China. Associated morphological characteristics and reference to its DNA barcode are provided. Boreoheptagyia kurobebrevis (Sasa & Okazawa, 1992) is newly recorded from China based on both a male and female, with additional associated data on the DNA barcode of the male adult. A neighbor-joining tree based on available Boreoheptagyia DNA barcodes and a key to the adults of Boreoheptagyia from China are given.

Mitogenomes provide new insights of evolutionary history of Boreheptagyiini and Diamesini (Diptera: Chironomidae: Diamesinae)

“…The cytochrome c oxidase I (COI) barcode sequence for each species was obtained by Sanger sequencing herein and from previous study (Lin, Yu, et al, 2021 ), and served as the “bait” references to acquire the best‐fit and targeted mitochondrial contigs by BLAST (Altschul et al, 1990 ) search in Geneious 2020.2.1 (Kearse et al, 2012 ). Moreover, clean reads were mapped onto the obtained mitogenome using Geneious to check the accuracy of the assembly.…”

“…The DNA and vouchers of the species are deposited at the college of Life Sciences, Nankai University, Tianjin, China. Specimens were identified morphologically using relevant taxonomic revisions and species descriptions (Lin, Chang, et al, 2021; Lin, Yu, et al, 2021; Makarchenko et al, 2008, 2021; Makarchenko & Wang, 2017; Moubayed‐Breil & Orsini, 2016; Oliver, 1983, 1989; Reiss, 1968; Sun et al, 2019), belonging to two tribes of Diamesinae.…”

DNA barcodes and morphology reveal new species within the Rheotanytarsus guineensis species group from China (Diptera: Chironomidae)

“…The DNA barcode, corresponding to the 658‐bp fragment of the 5′‐end of the mitochondrial gene cytochrome c oxidase I (COI), has been identified as the core of a global bio‐identification system at the species level and it has been considered the most commonly used marker for identifying nonbiting midge species delimitation (Hebert, Cywinska, et al, 2003; Hebert, Ratnasingham, et al, 2003). COI barcodes have provided important evidence to confirm new species (Anderson et al, 2013; Ekrem et al, 2018; Gadawski et al, 2022; Han et al, 2020, 2023; Kodama et al, 2018; Lin et al, 2015, 2018, 2019, 2020; Lin, Jiang, et al, 2021; Lin, Mo, et al, 2021; Lin et al, 2021; Song et al, 2018; Stur et al, 2019). In the R. guineensis group, DNA barcodes for only one species ( Rheotanytarsus ringei Lehmann, 1970) were reported in past studies.…”

New species, a new combination, and DNA barcodes of Parachironomus Lenz, 1921 (Diptera, Chironomidae)

“…The DNA barcodes corresponding to the 658-bp fragment of the mitochondrial gene cytochrome c oxidase I ( COI ) has been identified as the core of a global bio-identification system at the species level ( Hebert et al 2003a , b ). DNA barcodes also proved to be useful in the delimitation of non-biting midge species and has provided important evidence to confirm new species ( Anderson et al 2013 ; Lin et al 2015 , 2021 ; Giłka et al 2018 ; Liu et al 2021 ).…”