New observations on endometrial physiology after transcervical injection of methylene blue dye

Marconi, Guillermo; Vilela, M.; Quintana, Ramiro; Diradourian, M.; Young, E.; Sueldo, Carlos

doi:10.1016/j.fertnstert.2004.07.947

Cited by 13 publications

(10 citation statements)

References 7 publications

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“…Various studies were designed to assess the value of staining. Later, Marconi et al [11] also reported that endometrium is stained by methylene blue except periovulatory phase. The reason of endometrial staining is explained with apoptosis.…”

Section: Discussionmentioning

confidence: 97%

“Chromohysteroscopy” for evaluation of endometrium in recurrent in vitro fertilization failure

Küçük

Safalı

2008

J Assist Reprod Genet

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Purpose To assess the efficacy of chromohysteroscopy in detecting endometrial pathologies in recurrent in vitro fertilization (IVF) failure. Materials and methods Sixty-four patients in whom conventional hysteroscopy did not show any apparent endometrial pathology were included. Five milliliter of 1% methylene blue dye was introduced through the hysteroscopic inlet. Biopsies were obtained both from dark stained and light stained areas. Results The study group was grouped according to the staining characteristics. Group I included 22 patients in whom focal dark staining was observed. Group II included 41 patients in whom diffuse light blue staining without dark areas was observed. There was no significant difference between two groups in age, smoking, body mass index, number of IVF failure and time to hysteroscopy after the last failure. But, there was a statistically significant difference in the incidence of endometritis between two groups (p=0.007). The power of dark staining for detection of endometritis was calculated as follows: sensitivity 69.2%, specificity 74%, positive predictive value 40.9% and negative predictive value 90.2%. ConclusionChromohysteroscopy improves the efficacy of hysteroscopy in recurrent IVF failure. Observation of diffuse light blue staining without dark areas strongly suggests a normal endometrium free of endometritis.

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Section: Discussionmentioning

confidence: 97%

“Chromohysteroscopy” for evaluation of endometrium in recurrent in vitro fertilization failure

Küçük

Safalı

2008

J Assist Reprod Genet

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“…conducted the initial microhysteroscopic study, after a transcervical injection of MB into the endometrial cavity and observed changes in the endometrial surface during the various phases of the menstrual cycle . They correlated all microhysteroscopic images with the histologic findings from all EB samples . Other authors have explained endometrial staining with apoptosis and stated that structural damage of the cell during apoptosis would allow passage of the MB dye into the cell.…”

Section: Discussionmentioning

confidence: 99%

“…They have also conducted another study on MB dyeing during salpingoscopy and emphasized that the cell and its membrane had to be damaged for the MB to penetrate; in that study, epithelium had been damaged during the infection process . These authors have determined that the endometrium is not an absorptive epithelium in normal circumstances and structural damage of the cells allows passage of MB dye into the cells …”

Section: Discussionmentioning

confidence: 99%

“…15 These authors have determined that the endometrium is not an absorptive epithelium in normal circumstances and structural damage of the cells allows passage of MB dye into the cells. [13][14][15] Hysteroscopy might help identification of pathological findings missed by endometrial biopsy, like the atrophic mucosa. Endometrial sampling should be complementary to hysteroscopy in cases of abnormal uterine bleeding.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of two modalities: A novel technique, ‘chromohysteroscopy’, and blind endometrial sampling for the evaluation of abnormal uterine bleeding

Alay¹,

Usta

Ozay

et al. 2014

J of Obstet and Gynaecol

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Aim: The objective of this study was to compare classical blind endometrial tissue sampling with hysteroscopic biopsy sampling following methylene blue dyeing in premenopausal and postmenopausal patients with abnormal uterine bleeding. Material and Methods: A prospective case-control study was carried out in the Office Hysteroscopy Unit. Fifty-four patients with complaints of abnormal uterine bleeding were evaluated. Data of 38 patients were included in the statistical analysis. Three groups were compared by examining samples obtained through hysteroscopic biopsy before and after methylene blue dyeing, and classical blind endometrial tissue sampling. First, uterine cavity was evaluated with office hysteroscopy. Methylene blue dye was administered through the hysteroscopic inlet. Tissue samples were obtained from stained and non-stained areas. Blind endometrial sampling was performed in the same patients immediately after the hysteroscopy procedure. The results of hysteroscopic biopsy from methylene blue stained and non-stained areas and blind biopsy were compared. Results: No statistically significant differences were determined in the comparison of biopsy samples obtained from methylene-blue stained, non-stained areas and blind biopsy (P > 0.05). Conclusions:We suggest that chromohysteroscopy is not superior to endometrial sampling in cases of abnormal uterine bleeding. Further studies with greater sample sizes should be performed to assess the validity of routine use of endometrial dyeing.

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“…Chromohysteroscopy was first introduced after the study by Kucuk and Safali in 2008, where they combined chromo endoscopy and hysteroscopy as a new avenue to improve the diagnostic value of hysteroscopy (by using methylene blue dye) in the setting of assisted reproduction. 7 Endometritis is inflammation of the endometrial lining of the uterus. In addition to the endometrim, inflammation may involve the myometrium and occasionally the parametrium.…”

Section: Introductionmentioning

confidence: 99%

Chromohysteroscopy after failed intracytoplasmic sperm injection

Khalifa

Samra

Said

et al. 2016

Int J Reprod Contracept Obstet Gynecol

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New observations on endometrial physiology after transcervical injection of methylene blue dye

Cited by 13 publications

References 7 publications

“Chromohysteroscopy” for evaluation of endometrium in recurrent in vitro fertilization failure

“Chromohysteroscopy” for evaluation of endometrium in recurrent in vitro fertilization failure

Comparison of two modalities: A novel technique, ‘chromohysteroscopy’, and blind endometrial sampling for the evaluation of abnormal uterine bleeding

Chromohysteroscopy after failed intracytoplasmic sperm injection

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