New Non-opioid Analgesics: Understanding Molecular Mechanisms on the Basis of Patch-clamp and chemical Studies

Крылов, Б. В.; Rogachevskii, I. V.; Shelykh, Tatiana N.; Plakhova, Vera B.

doi:10.2174/97816080593001170101

Cited by 18 publications

(41 citation statements)

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“…В последние годы в литературе широко обсуждается вопрос о роли трансдукторной функции Na,K-АТФазы в клетке [13,14,20,56]. Вероятно, действие уабаина является не только ткане-, но и клеточно-специфичным.…”

Section: заключениеunclassified

“…Na,K-АТФаза-опосредованная сигнализация участвует во многих физиологических процессах, включая рост клеток, дифференцировку, апоптоз, воспаление, сократимость мышц и функцию почек [15][16][17][18][19]. В первичном сенсорном нейроне был обнаружен новый механизм мембранной сигнализации, запускаемый уабаином в концентрации, соответствующей его эндогенным значениям (эндогенный уабаин (ЭУ)), функцию трансдуктора сигнала в котором выполняет Na,K-ATФаза [8,20,21]. ЭУ синтезируется в коре надпочечников и гипоталамусе [10,22].…”

Section: Introductionunclassified

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О Молекулярной Природе Различий В Реакции Сенсорных Нейронов И Фибробластов На Уабаин

Халисов¹,

Пеннияйнен²,

Подзорова³

et al. 2021

Журнал Технической Физики

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Atomic force microscopy was used to study under physiologically adequate conditions the effect of ouabain on the mechanical characteristics of sensory neurons and fibroblasts of 10–12-day old chick embryos. Fibroblasts express only the α1-isoform of Na,K-ATPase, and sensory neurons the α1- and α3-isoforms. It was found that the action of ouabain at a concentration corresponding to the endogenous value leads to an increase in the membrane rigidity of sensory neurons, which is apparently due to the activation of the transducer function of Na,K-ATPase, rather than the pumping function. The endogenous concentration of ouabain did not change the mechanical characteristics of fibroblasts. The results obtained suggest that endogenous ouabain modulates the transducer function of the α3-isoform of Na,K-ATPase of the sensory neuron membrane. Thus, the method of atomic force microscopy allows a comparative study of intracellular signaling cascades in living cells.

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Section: заключениеunclassified

Section: Introductionunclassified

О Молекулярной Природе Различий В Реакции Сенсорных Нейронов И Фибробластов На Уабаин

Халисов¹,

Пеннияйнен²,

Подзорова³

et al. 2021

Журнал Технической Физики

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“…Na V 1.8 channels control nociceptive information transmitted to the central nervous system. It is known that agents that are capable of reducing the excitability of nociceptors by reducing the voltage-sensitivity of the activation gating device of the Na V 1.8 channels can claim to be analgesic drug substances [1]. We have obtained data indicating a possible mechanism underlying the relationship between the GABA-ergic and nociceptive systems [2].…”

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confidence: 99%

“…Patch-clamp whole-cell experiments allowed to investigate the voltage-sensitivity of nociceptive membrane as it was described earlier [1,3]. The culture of dissociated DRG sensory neurons isolated from the L5 -S1 domains of newborn Wistar rats makes it possible to study the effect of GABA on Na V 1.8 channels.…”

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confidence: 99%

“…The behavior of the activation gating device of the Na V 1.8 channels in response to the GABA applications was investigated using the Almers' method [1]. It is shown that the application of GABA from the outer side of the sensory neuron membrane at a concentration of 100 nM and 1 μM did not change the value of effective charge (Z eff ) of the activation gating system of the Na V 1.8 channels: Z eff = 6.5 ± 0.3 (n = 19, control data), and 6.3 ± 0.3 (n = 22), after 100 nM GABA application, and 6.4 ± 0.3 (n = 25) after 1 μM GABA application.…”

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The Gaba-Ergic System Does Not Control the Nociceptive Responses of Primary Sensory Neuron

Penniyaynen

Пеннияйнен²,

Подзорова

et al. 2019

Medical academic journal

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The aim of the study was to elucidate the molecular mechanisms of the interconnection of the GABA-ergic and nociceptive systems at the level of the peripheral division of the CNS. The data obtained indicate that GABA does not affect the activation gating device of the NaV1.8 channel of the primary sensory neuron responsible for coding pain signals.This agent in a wide range of concentrations also does not affect the growth of neurites of sensory neurons of embryonic nervous tissue. These results confirm our assumption, expressed earlier that the asynaptic membrane of the primary nociceptive neuron is not under the control of the GABA-ergic system.

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Possible Antinociceptive Mechanisms Triggered by Nanomolar Ouabain Concentrations in Primary Sensory Neurons

Penniyaynen

Khalisov

Подзорова

et al. 2021

Neurosci Behav Physi

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New Non-opioid Analgesics: Understanding Molecular Mechanisms on the Basis of Patch-clamp and chemical Studies

Cited by 18 publications

References 0 publications

О Молекулярной Природе Различий В Реакции Сенсорных Нейронов И Фибробластов На Уабаин

О Молекулярной Природе Различий В Реакции Сенсорных Нейронов И Фибробластов На Уабаин

The Gaba-Ergic System Does Not Control the Nociceptive Responses of Primary Sensory Neuron

Possible Antinociceptive Mechanisms Triggered by Nanomolar Ouabain Concentrations in Primary Sensory Neurons

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