New natural host of Southern rice black-streaked dwarf virus

Zhu, Jiayu; Zhou, Qian; Cui, Ya Cheng; Gao, Bingli

doi:10.3724/sp.j.1238.2012.00058

Cited by 10 publications

(11 citation statements)

References 2 publications

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“…Southern rice black-streaked dwarf virus can naturally infect a number of grasses, including rice, maize, Chinese sorghum ( Coix lacryma-jobi ), Avena fatua, Echinochloa crusgalli , Eleusine indica , and Pennisetum flaccidum (Zhou et al, 2008; Zhu et al, 2012). Diseased maize plants are clearly stunted with swollen internodes; densely clumped and darkened foliage; and wider, shorter, fragile leaves with ruffles.…”

Section: Pathogen and Transmissionmentioning

confidence: 99%

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Southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in Asia

Zhou¹,

Xu²,

Zhang³

2013

Front. Microbiol.

154

145

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Southern rice black-streaked dwarf virus (SRBSDV), a non-enveloped icosahedral virus with a genome of 10 double-stranded RNA segments, is a novel species in the genus Fijivirus (family Reoviridae) first recognized in 2008. Rice plants infected with this virus exhibit symptoms similar to those caused by Rice black-streaked dwarf virus. Since 2009, the virus has rapidly spread and caused serious rice losses in East and Southeast Asia. Significant progress has been made in recent years in understanding this disease, especially about the functions of the viral genes, rice–virus–insect interactions, and epidemiology and control measures. The virus can be efficiently transmitted by the white-backed planthopper (WBPH, Sogatella furcifera) in a persistent circulative propagative manner but cannot be transmitted by the brown planthopper (Nilaparvata lugens) and small brown planthopper (Laodelphax striatellus). Rice, maize, Chinese sorghum (Coix lacryma-jobi) and other grass weeds can be infected via WBPH. However, only rice plays a major role in the virus infection cycle because of the vector’s preference. In Southeast Asia, WBPH is a long-distance migratory rice pest. The disease cycle can be described as follows: SRBSDV and its WBPH vector overwinter in warm tropical or sub-tropical areas; viruliferous WBPH adults carry the virus from south to north via long-distance migration in early spring, transmit the virus to rice seedlings in the newly colonized areas, and lay eggs on the infected seedlings; the next generation of WBPHs propagate on infected seedlings, become viruliferous, disperse, and cause new disease outbreaks. Several molecular and serological methods have been developed to detect SRBSDV in plant tissues and individual insects. Control measures based on protection from WBPH, including seedbed coverage, chemical seed treatments, and chemical spraying of seedlings, have proven effective in China.

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Section: Pathogen and Transmissionmentioning

confidence: 99%

“…Diseased maize plants have short, small ears and always fail to fruit. On SRBSDV-infected Chinese sorghum, A. fatua, Echinochloa crusgalli , and Eleusine indica , dark green and ruffled leaves also can be observed (Zhou et al, 2008; Zhu et al, 2012). …”

Section: Pathogen and Transmissionmentioning

confidence: 99%

Southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in Asia

Zhou¹,

Xu²,

Zhang³

2013

Front. Microbiol.

154

145

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“…So far, RT‐PCR is given priority to the accurate diagnosis of SRBSDV (Zhou et al. , ; Ji et al. ; Wang et al.…”

Section: Discussionmentioning

confidence: 99%

“…As the outer capsid of SRBSDV particles is very fragile, and the virus is present in very low titers only in the phloem of the host plants (Zhou et al. , ), it is very difficult to obtain the specific antibody by virion purification. Wang et al.…”

Section: Discussionmentioning

confidence: 99%

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Development of SYBR Green I‐Based One‐Step Real Time RT‐PCR Assay for Quantifying Southern rice black‐streaked dwarf virus in Rice

Zhou

Lan

et al. 2013

Journal of Phytopathology

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Southern rice black‐streaked dwarf virus (SRBSDV) causes southern rice black‐streaked dwarf and maize rough dwarf diseases, which lead to severe yield losses of crops in Southeast Asia. We report here a SYBR Green I‐based One‐Step Real Time RT‐PCR assay for quantifying SRBSDV in rice rapidly and accurately. Primers used for assay were designed from the conserved sequence in S9 RNA among SRBSDV isolates. The RNA standards targeting the S9 region were obtained by transcription in vitro for generation of a standard curve. The assay developed in this study was found to be 100 times more sensitive than the conventional RT‐PCR for SRBSDV detection. The primers were very specific for SRBSDV. This study clearly demonstrated the potential usefulness of developed assay for detection and quantitation of SRBSDV in rice samples.

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Development of proteomic technology of shotgun and label free combined with multiple reaction monitoring to simultaneously detect southern rice black-streaked dwarf virus and rice ragged stunt virus

et al. 2014

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The co-infection of rice caused by southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) was widely found at many regions, such as Yunnan Province, China, and North and Central Vietnam. These rice viral diseases lead to seriously yield loss of rice. In this study, the proteomics technology of shotgun and label free combined with multiple reaction monitoring (MRM) was developed to detect rice sample of a single or/and co-infection. The shotgun assay indicated that some proteins coded by SRBSDV and RRSV were detected via the mode of in-gel digestion, except for P5-2, P7-2 and P9-2 of SRBSDV and P4b, P5, P6, P8a and P8b of RRSV. The technology of label free combined with MRM indicated that P2, P5-1, P4, P8, P7-1, P6, P9-1 and P10 of SRBSDV and P1, P3 and P9 of RRSV were higher abundance in rice plant, and P5-2, P7-2 and P9-2 of SRBSDV and P4b and P5 of RRSV were lower abundance in viruliferous-rice plant. So SRBSDV P9-1 and RRSV P3 was selected as marker molecule to be used in detection technology, and the label free combined with MRM technology was established to detect two kinds of rice virus.

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New natural host of Southern rice black-streaked dwarf virus

Cited by 10 publications

References 2 publications

Southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in Asia

Southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in Asia

Development of SYBR Green I‐Based One‐Step Real Time RT‐PCR Assay for Quantifying Southern rice black‐streaked dwarf virus in Rice

Development of proteomic technology of shotgun and label free combined with multiple reaction monitoring to simultaneously detect southern rice black-streaked dwarf virus and rice ragged stunt virus

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