New monoclonal antibody (4E9R) identifies mouse neural crest cells

Kubota, Yukihiko; Morita, Toshiteru; K, Ito

doi:10.1002/(sici)1097-0177(199608)206:4<368::aid-aja3>3.0.co;2-g

Cited by 24 publications

(22 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regions outside the brain enriched in γ3 mRNA include the trigeminal ganglia, of which the sensory component is derived from the mesencephalic neural crest (Chan and Tam, 1988) and dorsal root ganglia (DRG, spinal ganglia), a derivative of trunk (spinal) neural crest cells that colonize at day 9.5-10.5 p.c. (Kubota et al, 1996), and in the prospective ear, the latter confirmed in rat by Barritt et al (1999). Given the absolute conservation between the rat and mouse subunits (Fig.…”

Section: Spatial Expressionsupporting

confidence: 55%

Mouse G-protein gamma3 expression in the developing CNS and neural crest cell derivatives

Kelly¹,

Saijoh²,

Finkielsztein³

et al. 2008

Int. J. Dev. Biol.

View full text Add to dashboard Cite

show abstract

Section: Spatial Expressionsupporting

confidence: 55%

Mouse G-protein gamma3 expression in the developing CNS and neural crest cell derivatives

Kelly¹,

Saijoh²,

Finkielsztein³

et al. 2008

Int. J. Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Given the prominent NCC defect of mutants deficient in SEMA3A/NRP1 signaling, we next asked why an earlier report erroneously concluded that NCC migration in Nrp1-null mice was normal and that NRP1 was essential only to pattern the neuronal progeny of sympathetic NCCs (10). We noted that this previous work relied on a marker that labels blood vessels in addition to NCCs, the 4E9R antibody (20). We therefore addressed if mutant NCCs migrated in close proximity to blood vessels, as this would make them difficult to visualize with a dual specificity reagent such as 4E9R.…”

Section: Resultsmentioning

confidence: 99%

“…However, others suggested that NCC migration in Nrp1-null mice is normal and that NRP1 is essential only to pattern the neuronal progeny of sympathetic NCCs (10). This idea was derived from studies using the 4E9R antibody, which labels blood vessels in addition to NCCs (20). Because ectopic NCCs migrate in close association with blood vessels (Figs.…”

Section: Discussionmentioning

confidence: 99%

Neuropilin 1 signaling guides neural crest cells to coordinate pathway choice with cell specification

Schwarz

Maden

Vieira

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

142

View full text Add to dashboard Cite

Neural crest cells (NCCs) are highly motile embryonic stem cells that delaminate from the neuroectoderm early during vertebrate embryogenesis and differentiate at defined target sites into various essential cell types. To reach their targets, NCCs follow 1 of 3 sequential pathways that correlate with NCC fate. The firstborn NCCs travel ventrally alongside intersomitic blood vessels to form sympathetic neuronal progenitors near the dorsal aorta, while the lastborn NCCs migrate superficially beneath the epidermis to give rise to melanocytes. Yet, most NCCs enter the somites to form the intermediate wave that gives rise to sympathetic and sensory neurons. Here we show that the repulsive guidance cue SEMA3A and its receptor neuropilin 1 (NRP1) are essential to direct the intermediate wave NCC precursors of peripheral neurons from a default pathway alongside intersomitic blood vessels into the anterior sclerotome. Thus, loss of function for either gene caused excessive intersomitic NCC migration, and this led to ectopic neuronal differentiation along both the anteroposterior and dorsoventral axes of the trunk. The choice of migratory pathway did not affect the specification of NCCs, as they retained their commitment to differentiate into sympathetic or sensory neurons, even when they migrated on an ectopic dorsolateral path that is normally taken by melanocyte precursors. We conclude that NRP1 signaling coordinates pathway choice with NCC fate and therefore confines neuronal differentiation to appropriate locations.semaphorin ͉ sensory neuron ͉ sympathetic neuron ͉ peripheral nervous system

show abstract

“…Immunostainings of embryos and organ culture explants were performed as described previously (Kubota et al, 1996(Kubota et al, , 1999Kubota and Ito, 2000). Embryos or explants were fixed with 4% paraformaldehyde (PFA) in phosphate-buffered saline (pH 7.4) or with methanol containing 0.1% formaldehyde at Ϫ20°C for 10 min in staining using a rat monoclonal antibody, 4E9R.…”

Section: Immunostainingmentioning

confidence: 99%

“…Cryostat sections were cut at 15 m and mounted on albumin-coated glass slides. The following primary antibodies were used: (1) mouse monoclonal anti-Islet-1, 39.4D5 (1:100; Developmental Studies Hybridoma Bank); (2) goat anti-NeuroD (1:100; Santa Cruz); (3) mouse monoclonal anti-Brn3a (1:100; Santa Cruz); (4) mouse monoclonal antiShh, 5E1 (1:10; Developmental Studies Hybridoma Bank); (5) goat antiPatched (1:20; Santa Cruz) to detect the Shh receptor Patched (McMahon, 2000); and (6) rat monoclonal 4E9R to stain migrating mouse neural crest cells (Kubota et al, 1996). Primary antibodies were applied to cryosections at 4°C for 12 hr.…”

Section: Immunostainingmentioning

confidence: 99%

Induction of neurogenin‐1 expression by sonic hedgehog: Its role in development of trigeminal sensory neurons

Ota

2003

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

We have examined the roles of signaling molecules in the mechanisms underlying the induction of neurogenin (ngn)-1 expression. ngn-1 is a basic helix-loop-helix (bHLH) transcription factor, which is essential for the specification of trigeminal sensory neurons. Semiquantitative reverse transcriptase-polymerase chain reaction using cranial explants in organ cultures showed that sonic hedgehog (Shh) promotes ngn-1 expression. This promoting activity was not observed in other signaling molecules examined. The promotion of ngn-1 expression by Shh, furthermore, was inhibited by cyclopamine, a specific inhibitor of Shh signaling. Shh did not affect the expression of ngn-2, a bHLH transcription factor that plays an important role in the specification of epibranchial placode-derived sensory neurons.

show abstract

New monoclonal antibody (4E9R) identifies mouse neural crest cells

Cited by 24 publications

References 38 publications

Mouse G-protein gamma3 expression in the developing CNS and neural crest cell derivatives

Mouse G-protein gamma3 expression in the developing CNS and neural crest cell derivatives

Neuropilin 1 signaling guides neural crest cells to coordinate pathway choice with cell specification

Induction of neurogenin‐1 expression by sonic hedgehog: Its role in development of trigeminal sensory neurons

Contact Info

Product

Resources

About