New Molecular Mechanism of Superbiofilm Elaboration in a Staphylococcus aureus Clinical Strain

Yu, Liansheng; Hisatsune, Junzo; Kutsuno, Shoko; Sugai, Motoyuki

doi:10.1128/spectrum.04425-22

Cited by 3 publications

(1 citation statement)

References 30 publications

(44 reference statements)

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“…For instance, expression of icaA was unaffected by ethanol directly; however, it increased by repressing icaR transcription. Conversely, the induction of icaA expression by glucose or NaCl was icaR independent [ 29 ]. Thus, the study of these genetic markers could further lead to the design of new drugs aimed at biofilm inhibition by inducing the activity of all or some icaADBC operon repressors.…”

Section: Introductionmentioning

confidence: 99%

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

González-Vázquez,

Córdova-Espinoza,

Escamilla-Gutiérrez

et al. 2024

Pathogens

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Methicillin-resistant (MR) Staphylococcus aureus (SA) and others, except for Staphylococcus aureus (SOSA), are common in healthcare-associated infections. SOSA encompass largely coagulase-negative staphylococci, including coagulase-positive staphylococcal species. Biofilm formation is encoded by the icaADBC operon and is involved in virulence. mecA encodes an additional penicillin-binding protein (PBP), PBP2a, that avoids the arrival of β-lactams at the target, found in the staphylococcal cassette chromosome mec (SCCmec). This work aims to detect mecA, the bap gene, the icaADBC operon, and types of SCCmec associated to biofilm in MRSA and SOSA strains. A total of 46% (37/80) of the strains were S. aureus, 44% (35/80) S. epidermidis, 5% (4/80) S. haemolyticus, 2.5% (2/80) S. hominis, 1.25% (1/80) S. intermedius, and 1.25% (1/80) S. saprophyticus. A total of 85% were MR, of which 95.5% showed mecA and 86.7% β-lactamase producers; thus, Staphylococcus may have more than one resistance mechanism. Healthcare-associated infection strains codified type I-III genes of SCCmec; types IV and V were associated to community-acquired strains (CA). Type II prevailed in MRSA mecA strains and type II and III in MRSOSA (methicillin-resistant staphylococci other than Staphylococcus aureus). The operon icaADBC was found in 24% of SA and 14% of SOSA; probably the arrangement of the operon, fork formation, and mutations influenced the variation. Methicillin resistance was mainly mediated by the mecA gene; however, there may be other mechanisms that also participate, since biofilm production is related to genes of the icaADBC operon and methicillin resistance was not associated with biofilm production. Therefore, it is necessary to strengthen surveillance to prevent the spread of these outbreaks both in the nosocomial environment and in the community.

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Section: Introductionmentioning

confidence: 99%

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

González-Vázquez,

Córdova-Espinoza,

Escamilla-Gutiérrez

et al. 2024

Pathogens

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Effects of the quorum sensing related luxS gene and lsr operon on Klebsiella michiganensis resisting copper stress

Gao,

Peng,

Wang

et al. 2024

Environmental Research

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Various mutations in icaR, the repressor of the icaADBC locus, occur in mucoid Staphylococcus aureus isolates recovered from the airways of people with cystic fibrosis

Schwartbeck,

Rumpf,

Hait

et al. 2024

Microbes and Infection

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New Molecular Mechanism of Superbiofilm Elaboration in a Staphylococcus aureus Clinical Strain

Abstract: Poly- N -acetylglucosamine (PNAG) is a major component of S. aureus biofilm. PNAG production is mediated by the products of four genes, icaADBC encoded in the ica operon, and the major negative regulator of this operon is IcaR encoded just upstream of icaADBC .

Cited by 3 publications

References 30 publications

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

Effects of the quorum sensing related luxS gene and lsr operon on Klebsiella michiganensis resisting copper stress

Various mutations in icaR, the repressor of the icaADBC locus, occur in mucoid Staphylococcus aureus isolates recovered from the airways of people with cystic fibrosis

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