New Molecular Markers Linked with the High Shoot Regeneration Capacity of the Wild Tomato Species Lycopersicon chilense.

Takashina, Tadashi; Suzuki, Toshiyuki; Egashira, Hiroaki; Imanishi, Shigeru

doi:10.1270/jsbbs1951.48.109

Cited by 17 publications

(18 citation statements)

References 23 publications

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“…Hence, it is possible to introgress genes for high in vitro regeneration capability from a wild P2 to recalcitrant eggplant cultivars like P5 and P3. This is in agreement with the earlier reports on the genetic control of regeneration and introgression of genes responsible to high regeneration capability from wild to recalcitrant tomato cultivars (Frankenberger et al 1981;Koornneef et al 1987;Wijbrandi et al 1988;Faria and Illg 1996;Takashina et al 1998;Faria et al 2002;Marchionni et al 2007). …”

Section: Discussionsupporting

confidence: 93%

Genetic analysis of in vitro callus and production of multiple shoots in eggplant

Chakravarthi¹,

Rao

et al. 2010

Plant Cell Tiss Organ Cult

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Genetic analysis of four in vitro characters (callus initiation, callus productivity, embryogenic callus percentage, and mean number of regenerated shoots per callus) was conducted using a 6 9 6 diallel cross among four cultivars of eggplant (Solanum melongena) and two related species (S. indicum, S. surattense) showing different in vitro responses. Among the 30 hybrid families, the in vitro performance exceeded the midparent in 19, 29, 18, and 22 cases, respectively, for callus initiation, callus productivity, embryogenic callus induction, and number of regenerated shoots. Both additive and dominant effects were significant and additive gene action was predominant for three of four in vitro characters with the exception of callus productivity. Different degrees of dominance were observed in the expression of the four characters. The broad and narrow sense heritabilities were 0.97 and 0.82 for callus initiation frequency, 0.99 and 0.42 for total amount of callus, 0.99 and 0.92 for E-callus productivity, and 0.99 and 0.73 for mean number of shoots, respectively, suggesting that these traits can be easily transferred into economically important cultivars with low tissue culture response or recalcitrance.

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Section: Discussionsupporting

confidence: 93%

Genetic analysis of in vitro callus and production of multiple shoots in eggplant

Chakravarthi¹,

Rao

et al. 2010

Plant Cell Tiss Organ Cult

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“…Previously reported molecular markers were associated with increasing the in vitro shoot formation (Koornneef et al 1993, Torelli et al 1996, Takashina et al 1998. The AFLP markers detected in the present research were associated with a reduction in the regeneration capacity of the RILs.…”

Section: ⎯⎯⎯⎯supporting

confidence: 59%

“…Koornneef et al (1993) mapped a high regeneration QTL to chromosome III in an interspecific tomato cross by RFLP analysis. Torelli et al (1996) detected by the differential display technique, some specific mRNA transcripts expressed during the earlier incubation period of tomato explants that were associated to the shoot formation capacity, while Takashina et al (1998) found at least two RAPD and one isoenzymatic markers linked to the high regeneration capacity of the wild L. chilense.The objective of this research was to evaluate both the in vitro callus production and shoot formation, and to detect cosegregating AFLP markers in a set of elite tomato genotypes. Our ultimate goal was to contribute to the knowledge of the inheritance of in vitro culture response of the tomato.…”

mentioning

confidence: 98%

Variability for the in vitro culture response in tomato recombinant inbred lines

Pratta¹,

Zorzoli²,

Picardi³

et al. 2006

Biol. plant.

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The aim of this work was to estimate genetic variability for in vitro culture response of recombinant inbred lines (RILs) of the genus Lycopersicon. The callus percentage (C), the regeneration percentage (R) and the productivity rate (PR) were evaluated 45 d after culture initiation in a set of 16 elite tomato RILs and their parents. The narrow sense heritability (h 2 ) values were 0.38 ± 0.04 for C, 0.46 ± 0.04 for R, and 0.28 ± 0.03 for R, while the genetic correlation (r g ) values were -0.96 ± 0.07 between C and R, 0.81 ± 0.14 between PR and R, and -0.79 ± 0.16 between PR and C. Three AFLP markers associated to the in vitro traits were identified.Additional key words: amplified fragment length polymorphism (AFLP), callus production, Lycopersicon esculentum, Lycopersicon pimpinellifolium, plant breeding, shoot formation. ⎯⎯⎯⎯Intra and interspecific variability for callus proliferation and shoot regeneration has been widely reported in the genus Lycopersicon (Tal et al. 1977, Pratta et al. 1997. Dedifferentiation of leaf explants into a callus, either followed or not by shoot formation, was dependent on genotype, culture medium and physiological stage of the donor plants. Although genetic control of in vitro culture traits was investigated in various crops (Kuroda et al. 1998, Nestares et al. 1998, Ogburia 2003) there is not enough information about the inheritance of callus production and shoot formation in the cultivated tomato (Lycopersicon esculentum Mill.) (Frankerberger et al. 1981, Pratta et al. 2003. Reports on molecular markers associated to the tomato in vitro culture responses are even scarcer. Koornneef et al. (1993) mapped a high regeneration QTL to chromosome III in an interspecific tomato cross by RFLP analysis. Torelli et al. (1996) detected by the differential display technique, some specific mRNA transcripts expressed during the earlier incubation period of tomato explants that were associated to the shoot formation capacity, while Takashina et al. (1998) found at least two RAPD and one isoenzymatic markers linked to the high regeneration capacity of the wild L. chilense.The objective of this research was to evaluate both the in vitro callus production and shoot formation, and to detect cosegregating AFLP markers in a set of elite tomato genotypes. Our ultimate goal was to contribute to the knowledge of the inheritance of in vitro culture response of the tomato.Seeds of 16 elite tomato recombinant inbred lines (RILs) and their parents (Lycopersicon esculentum (L.) Mill. cv. Caimanta and L. pimpinellifolium Mill. LA722, included as testers) were sown in seedling trays under greenhouse conditions at the field station "José F. Villarino" (Facultad de Ciencias Agrarias UNR, Zavalla, Argentina, 33°S and 61°W). RILs were the F 7 filial generation (more than 99 % homozygotes) from the interspecific cross Caimanta × LA722. They were developed through a breeding program to obtain long shelf life and high mass tomato genotypes through divergent and antagonic selection. In vitro culture was performed acc...

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“…In addition, a putative allele of Rg-1 from S . chilense (Dunal) Reiche ( Rg-2 ) was reported by Takashina et al [9] and Satoh et al [22]. Both alleles may act in combination with other alleles of either tomato or the wild relatives S. peruvianum or S. chilense [22,25].…”

Section: Introductionmentioning

confidence: 99%

Localization of QTLs for in vitroplant regeneration in tomato

et al. 2011

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BackgroundLow regeneration ability limits biotechnological breeding approaches. The influence of genotype in the regeneration response is high in both tomato and other important crops. Despite the various studies that have been carried out on regeneration genetics, little is known about the key genes involved in this process. The aim of this study was to localize the genetic factors affecting regeneration in tomato.ResultsWe developed two mapping populations (F2 and BC1) derived from a previously selected tomato cultivar (cv. Anl27) with low regeneration ability and a high regeneration accession of the wild species Solanum pennellii (PE-47). The phenotypic assay indicated dominance for bud induction and additive effects for both the percentage of explants with shoots and the number of regenerated shoots per explant. Two linkage maps were developed and six QTLs were identified on five chromosomes (1, 3, 4, 7 and 8) in the BC1 population by means of the Interval Mapping and restricted Multiple QTL Mapping methods. These QTLs came from S. pennellii, with the exception of the minor QTL located on chromosome 8, which was provided by cv. Anl27. The main QTLs correspond to those detected on chromosomes 1 and 7. In the F2 population, a QTL on chromosome 7 was identified on a similar region as that detected in the BC1 population. Marker segregation distortion was observed in this population in those areas where the QTLs of BC1 were detected. Furthermore, we located two tomato candidate genes using a marker linked to the high regeneration gene: Rg-2 (a putative allele of Rg-1) and LESK1, which encodes a serine/threonine kinase and was proposed as a marker for regeneration competence. As a result, we located a putative allele of Rg-2 in the QTL detected on chromosome 3 that we named Rg-3. LESK1, which is also situated on chromosome 3, is outside Rg-3. In a preliminary exploration of the detected QTL peaks, we found several genes that may be related to regeneration.ConclusionsIn this study we have identified new QTLs related to the complex process of regeneration from tissue culture. We have also located two candidate genes, discovering a putative allele of the high regeneration gene Rg-1 in the QTL on chromosome 3. The identified QTLs could represent a significant step toward the understanding of this process and the identification of other related candidate genes. It will also most likely facilitate the development of molecular markers for use in gene isolation.

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New Molecular Markers Linked with the High Shoot Regeneration Capacity of the Wild Tomato Species Lycopersicon chilense.

Cited by 17 publications

References 23 publications

Genetic analysis of in vitro callus and production of multiple shoots in eggplant

Genetic analysis of in vitro callus and production of multiple shoots in eggplant

Variability for the in vitro culture response in tomato recombinant inbred lines

Localization of QTLs for in vitroplant regeneration in tomato

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