New molecular data for parasites Hammerschmidtiella indicus and Thelandros scleratus (Nematoda: Oxyurida) to infer phylogenetic position

Abstract

A phylogenetic study of nematode species belonging to Nematoda: Oxyurida from India has been conducted using molecular characters. Molecular marker 18S rRNA (18S) from the nuclear gene was tested and analyses were conducted using the minimum evolution, maximum parsimony, and maximum likelihood methods. Phylogenetic analysis revealed that both of the species Hammerschmidtiella indicus and Thelandros scleratus clustered as sister species with species of Thelastoma and Leidynema, and Parapharyngodon, respectively. Interestingly, the results confirm the taxonomic status of H. indicus and T. scleratus from India.

Molecular and ultrastructure characterization of two nematodes(Thelandros scleratus and Physalopteroides dactyluris)based on ribosomal and mitochondrial DNA sequences

“…The phylogenetic tree also groups closely T. scleratus and Parapharyngodon. This supports the previous fi ndings which already pointed on the closeness of these two genera (Chaudhary et al, 2014). In fact, the two genera are morphologically very similar (Mašová et al, 2008).…”

“…Status of Thelandros species in India has also been questionable due to lack of molecular tools. Some phylogenetic studies of T. scleratus have been carried out with nuclear genes like 18S and 28S (Kumari et al, 2011;Chaudhary et al, 2014). The genus Physalopteroides was recognized in China by Wu & Liu, 1940 with the type species of P. dryophisi collected from Dryophis prasinus.…”

Procamallanus spiculogubernaculus Agarwal, 1958 (Nematoda: Camallanidae) from Stinging catfish, Heteropneustes fossilis in India: morphological characterization and molecular data

“…By comparing the sequence differences, it is easy to identify closely related nematode species. The molecular markers based on nuclear and mitochondrial genes are an excellent tool for differentiating nematode species (Floyd et al, 2002;Blaxter et al, 2005;McLean et al, 2012;Singh et al, 2013;Nakacwa et al, 2013;Chaudhary et al, 2015;Mikaeili et al, 2015;Goswami et al, 2015). The present molecular analyses using 18S rDNA and cox1 mtDNA genes support the placement of P. spiculogubernaculus in family Camallanidae.…”

Molecular phylogeny of nematodes (Oxyurida: Travassosinematidae) from orthoptera (Gryllotalpidae) inferred by mitochondrial cytochrome C oxidase subunit 1 gene

“…Several parasitological examinations from India have been conducted on the basis of morphology of travassosinematids. Majority of nematode species infecting G. africana have been described only by the morphology except few studies [ 35 , 36 ]. The descriptions available were based on the morphology of mouth parts, oesophagus structures, tail, male and female reproductive organs and sometimes were not sufficiently informative.…”