New model of renal warm ischaemia–reperfusion injury for comparative functional, morphological and pathophysiological studies

Introduction: Renal ischemia-reperfusion (I/R) injury is a primary cause of acute renal failure that results in high mortality. This study aimed to investigate the effect of osthole, a natural coumarin derivative, on renal I/R injury in a rat model. Materials and Methods: Rats were randomly allocated to the sham operation + vehicle, I/R + vehicle, and I/R + osthole groups. Renal I/R injury was induced by clamping the left renal artery for 45 min followed by 12 h of reperfusion and a contralateral nephrectomy. Osthole (40 mg/kg) was intraperitoneally injected 30 min before inducing I/R. Renal function and histological damage were determined subsequently. Myeloperoxidase activity, monocyte/macrophage infiltration, as well as tumor necrosis factor-α, IL-1β, and activated p38 mitogen-activated protein kinase expression in kidneys were also assessed. Results: Osthole treatment significantly ameliorated I/R-induced renal functional and morphological injuries. Moreover, osthole treatment attenuated myeloperoxidase activity, monocyte/macrophage infiltration, and tumor necrosis factor-α, IL-1β, and activated p38 mitogen-activated protein kinase expression in kidneys. Conclusions: Osthole treatment ameliorates renal I/R injury by inhibiting inflammatory responses in kidneys. Thus, osthole may represent a novel practical strategy to prevent renal I/R injury.

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“…Renal I/R injury was induced as previously described [8]. Briefly, a midline laparotomy incision was made, followed by a right nephrectomy.…”

Section: Methodsmentioning

confidence: 99%

Osthole Ameliorates Renal Ischemia-Reperfusion Injury by Inhibiting Inflammatory Response

Zheng

Lü²,

Ma³

et al. 2013

Urol Int

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“…In 8/31 publications, the endto-end technique was described. 5,14,16,19,20,22,24,25,35 In 16 publications, the authors favored the end-to-side technique, 1,6À9,11,12,15,17,20,26À29,30,34 and in 6 publications, the sleeve technique 13,18,23,31À33 was favored. Because of the dissection of the shorter renal vessels, the end-to-side technique had to be used in cases of right-sided anastomoses.…”

Section: Surgical Techniquesmentioning

confidence: 96%

“…It is generally believed that the ''warm ischemic time'' must be less than 30 min, and a number of investigators attempted to reduce this time in an effort to minimize ischemic damage. 35 Weight et al demonstrated good correlation between increasing duration of warm ischemic time and degree of renal impairment, both functionally and morphologically. 35 On day 2 there was progressive impairment in the glomerular filtration rate (GFR), 4 with increasing duration of warm ischemic time.…”

Section: Outcomementioning

confidence: 97%

“…35 Weight et al demonstrated good correlation between increasing duration of warm ischemic time and degree of renal impairment, both functionally and morphologically. 35 On day 2 there was progressive impairment in the glomerular filtration rate (GFR), 4 with increasing duration of warm ischemic time. This was found to be significant in the 30-min, 45-min, and 60-min warm ischemic groups.…”

Section: Outcomementioning

confidence: 97%

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Kidney transplantation in rats: An appraisal of surgical techniques and outcome

2003

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Renal transplantation in rats is an essential experimental tool in transplantation research. The surgical procedure per se could affect the outcome of an experiment, independent of the hypothesis addressed, therefore requiring a standardized method which should be comparable across studies. To date, however, there is little information on the optimal surgical technique. We performed a Medline search on original articles published between 1965-2001 in order to evaluate whether specific technical issues affecting the outcome of the procedure could be defined. Articles that reported on a novel microsurgical procedure, or whose main purpose was the outcome of a surgical technique itself, were included in the analysis. From 2,060 retrieved publications, 34 corresponded to the selection criteria (rats and microsurgery and technique and kidney or renal transplantation). Among the essential determining factors for a good outcome, body weight >200 g and warm ischemic time <30 min were identified. Other important factors were the techniques used for vascular (end-to-end and end-to-side procedure or sleeve technique) and ureteral (bladder patch or end-to-end procedure) anastomosis. Gender, animal strain, type of anesthesia, prophylactic administration of antibiotics, and type of flushing solution did not affect the success of renal allografts. In order to avoid a bias related to the surgical procedure in rat renal transplantation, a warm ischemia time <30 min in animals with a body weight >200 g seems to be essential. Also, end-to-end or end-to-side vascular anastomoses are preferable to the sleeve technique. Other factors do not influence the immediate function of the graft.

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“…The renal IRI model was previously described elsewhere [17]. In brief, a midline abdominal incision was made, followed by right nephrectomy.…”

Section: Experimental Protocol and Renal Iri Modelmentioning

confidence: 99%

Protective Effects of Butyrate on Renal Ischemia-Reperfusion Injury in Rats

Zheng

Zhang

2019

Urol Int

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Background: Renal ischemia-reperfusion injury (IRI) usually causes acute kidney injury. There is an urgent need to develop an effective agent to prevent renal IRI. This study aimed to examine the effect of butyrate on renal IRI in rats. Materials and Methods: Rats were randomly assigned into 3 groups (10 rats in each group): the sham group, the IRI group, and the butyrate group. Rats were injected intravenously with 300 mg/kg of sodium butyrate in the butyrate group and with a saline solution in the sham group and IRI group 30 min before renal ischemia. After 24 h of reperfusion, renal function and histologic damage were examined. Myeloperoxidase (MPO) activity assay, in situ apoptosis examination, enzyme-linked immunosorbent assay, immunohistochemical assay, and Western blot were performed as well. Results: Butyrate pretreatment significantly reduced renal dysfunction and histologic damage induced by renal IRI. Butyrate pretreatment caused a significant attenuation of neutrophil infiltration, which was reflected by the reduction of renal MPO activity. Butyrate also reduced apoptotic tubular cell death and improved caspase-3 activation. The expression of TNF-α was decreased following butyrate pretreatment. Conclusions: Butyrate pretreatment protects rats from renal IRI by inhibiting inflammation and apoptosis. Therefore, butyrate may be a potential therapeutic agent for preventing renal IRI.

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New model of renal warm ischaemia–reperfusion injury for comparative functional, morphological and pathophysiological studies

Abstract: This new model allowed comparative functional, morphological and pathophysiological studies while minimizing the number of animals required. Overall 45 min of warm ischaemia gave significant, recoverable injury and is recommended for investigating renal reperfusion injury.

Cited by 40 publications

References 11 publications

Osthole Ameliorates Renal Ischemia-Reperfusion Injury by Inhibiting Inflammatory Response

Osthole Ameliorates Renal Ischemia-Reperfusion Injury by Inhibiting Inflammatory Response

Kidney transplantation in rats: An appraisal of surgical techniques and outcome

Protective Effects of Butyrate on Renal Ischemia-Reperfusion Injury in Rats

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