New microsatellite markers for Dacryodes edulis (Burseraceae), an indigenous fruit tree species from Central Africa

Rimlinger, Aurore; Marie, Lison; Avana, Marie-Louise; Bouka, Gaël; Zekraoui, Leïla; Mariac, Cédric; Carrière, Stéphanie M.; Duminil, Jérôme

doi:10.1007/s11033-020-05270-4

Cited by 6 publications

(7 citation statements)

References 14 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We extracted DNA from dried leaves corresponding to 455 trees (among which 402 trees with complete fruit morphology are available) following the protocol from Mariac et al (2006) and genotyped them using 10 out of 21 nuclear microsatellites available for the species, selecting the most polymorphic and unambiguous (DaE‐16, DaE‐20, DaE‐23, DaE‐27, DaE‐29, DaE‐36, DaE‐41, DaE‐42, DaE‐46, DaE‐49; Rimlinger et al, 2020). Individuals were genotyped at the CIRAD Genotyping Platform in Montpellier, France, using an ABI 3500 XL sequencer (Applied Biosystem, Foster City, California, USA).…”

Section: Methodsmentioning

confidence: 99%

Can seed exchange networks explain the morphological and genetic diversity in perennial crop species? The case of the tropical fruit tree Dacryodes edulis in rural and urban Cameroon

Rimlinger,

Duminil,

Avana‐Tientcheu

et al. 2023

Plants People Planet

Self Cite

View full text Add to dashboard Cite

Societal Impact StatementCrop seed exchange networks, shaped by social dynamics, strongly influence the organization and breadth of plant diversity in human‐managed environments. Integrating an urban and market perspective, this study explores the diversity dynamics of a socio‐economically important Central African fruit tree species, the African plum tree. Tree owners in urban, peri‐urban and rural sites use seeds from different origins as their main propagation material, leading to locational variations in genetic diversity and structure. This analysis contributes toward building a framework to inform the research agenda of cultivated African fruit trees, by highlighting the important role of urban centers in safeguarding crop genetic resources.Summary Biocultural factors constrain the dynamics of crop species diversity. Here, we considered different aspects of the social, spatial and temporal dynamics of morphological and genetic diversity in a multi‐purpose perennial crop, the African plum tree (Dacryodes edulis). We assessed (i) how seed exchange networks were organized along urbanization gradients, and how they influenced the distribution of species diversity; (ii) the temporal dynamic of seed exchange network by characterizing species genetic diversity through time. To do so, the study was carried out in Cameroon, where we focused on three urbanization gradients, covering urban, peri‐urban and rural areas, corresponding to three different ethnic groups (Bamileke, Bassa, Beti). We combined interviews with tree owners and nuclear microsatellite‐based genetic analyses. Tree owners from urban and peri‐urban sites primarily used distant seed sources, acquired in the market or from their village of origin, as propagation material, whereas tree owners in rural sites relied primarily on village‐level seeds. In turn, genetic diversity was not evenly distributed, with rural sites exhibiting their own genetic clusters. On the contrary, the genetic diversity of urban sites was enhanced by extensive human‐mediated seed flows. Looking at trees from different age classes, we found that genetic diversity was stable over time. Overall, this first attempt to combine different levels of diversity for African plum trees in commercially connected areas expands the scope for in situ intraspecific conservation by highlighting the contribution of urbanized areas.

show abstract

Section: Methodsmentioning

confidence: 99%

Can seed exchange networks explain the morphological and genetic diversity in perennial crop species? The case of the tropical fruit tree Dacryodes edulis in rural and urban Cameroon

Rimlinger,

Duminil,

Avana‐Tientcheu

et al. 2023

Plants People Planet

Self Cite

View full text Add to dashboard Cite

show abstract

“…The genetic diversity was investigated using twelve nuclear microsatellite markers amplified in two multiplexes following Rimlinger et al . [43]. All the individuals were genotyped using an ABI 3500 XL sequencer (Applied Biosystem, Foster City, California, USA) at the CIRAD Genotyping Platform in Montpellier, France.…”

Section: Methodsmentioning

confidence: 99%

“…We extracted the DNA of these 849 urban and rural samples following the protocol of Mariac et al [42] (S1 Dataset). The genetic diversity was investigated using twelve nuclear microsatellite markers amplified in two multiplexes following Rimlinger et al [43]. All the individuals were genotyped using an ABI 3500 XL sequencer (Applied Biosystem, Foster…”

Section: Genetic Data Collection and Analysismentioning

confidence: 99%

Trees and their seed networks: the social dynamics of urban fruit trees and implications for genetic diversity

Rimlinger¹,

Avana

Awono³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Trees are a traditional component of urban spaces where they provide ecosystem services critical to urban wellbeing. In the Tropics, urban trees’ seed origins have rarely been characterized. Yet, understanding the social dynamics linked to tree planting is critical given their influence on the distribution of associated genetic diversity. This study examines elements of these dynamics (seed exchange networks) in an emblematic indigenous fruit tree species from Central Africa, the African plum tree (Dacryodes edulis, Burseraceae), within the urban context of Yaoundé. We further evaluate the consequences of these social dynamics on the distribution of the genetic diversity of the species in the city. Urban trees were planted predominantly using seeds sourced from outside the city, resulting in a level of genetic diversity as high in Yaoundé as in a whole region of production of the species. Debating the different drivers that foster the genetic diversity in planted urban trees, the study argued that cities and urban dwellers can unconsciously act as effective guardians of indigenous tree genetic diversity.

show abstract

“…In D. edulis, six microsatellite markers were used for niche characterization, gene flow and genetic structuration in selected Cameroonian and Gabonese populations and accessions [291,[311][312][313]. However, these markers failed to segregate clusters and ethnovarietal groups due to the outcrossing reproduction systems of the species added to the social networks, which strengthened gene flow and contributed to an unexpected high level of genetic diversity conservation within and among populations, albeit affected by the selection intensity placed on preferred traits [242,245,314]. Recommendations for next steps in genetic studies include the isolation and characterization of more DNA markers in D. edulis and their use for early selection in progeny trials and advanced studies such as gene discovery, trait-linked marker-assisted selection and gene mapping [291,312,314].…”

Section: Molecular Studiesmentioning

confidence: 99%

“…Most studies on genetic variation during the third decade have been focussed on assessing the genetic and social consequences of the morphological diversity observed in preferred fruits traits among trees within and between populations, such as in D. edulis [245,314].…”

Section: Selection Of Elite Trees and Ideotypesmentioning

confidence: 99%

The Future of Food: Domestication and Commercialization of Indigenous Food Crops in Africa over the Third Decade (2012–2021)

Leakey¹,

Avana

Awazi

et al. 2022

Sustainability

View full text Add to dashboard Cite

This paper follows the transition from ethnobotany to a deeper scientific understanding of the food and medicinal properties of African agroforestry tree products as inputs into the start of domestication activities. It progresses on to the integration of these indigenous trees as new crops within diversified farming systems for multiple social, economic and environmental benefits. From its advent in the 1990s, the domestication of indigenous food and non-food tree species has become a global programme with a strong African focus. This review of progress in the third decade is restricted to progress in Africa, where multi-disciplinary research on over 59 species has been reported in 759 research papers in 318 science publications by scientists from over 833 research teams in 70 countries around the world (532 in Africa). The review spans 23 research topics presenting the recent research literature for tree species of high priority across the continent, as well as that in each of the four main ecological regions: the humid zone of West and Central Africa; the Sahel and North Africa; the East African highlands and drylands; and the woody savannas of Southern Africa. The main areas of growth have been the nutritional/medicinal value of non-timber forest products; the evaluation of the state of natural resources and their importance to local people; and the characterization of useful traits. However, the testing of putative cultivars; the implementation of participatory principles; the protection of traditional knowledge and intellectual property rights; and the selection of elite trees and ideotypes remain under-researched. To the probable detriment of the upscaling and impact in tropical agriculture, there has been, at the international level, a move away from decentralized, community-based tree domestication towards a laboratory-based, centralized approach. However, the rapid uptake of research by university departments and national agricultural research centres in Africa indicates a recognition of the importance of the indigenous crops for both the livelihoods of rural communities and the revitalization and enhanced outputs from agriculture in Africa, especially in West Africa. Thus, on a continental scale, there has been an uptake of research with policy relevance for the integration of indigenous trees in agroecosystems and their importance for the attainment of the UN Sustainable Development Goals. To progress this in the fourth decade, there will need to be a dedicated Centre in Africa to test and develop cultivars of indigenous crops. Finally, this review underpins a holistic approach to mitigating climate change, as well as other big global issues such as hunger, poverty and loss of wildlife habitat by reaping the benefits, or ‘profits’, from investment in the five forms of Capital, described as ‘land maxing’. However, policy and decision makers are not yet recognizing the potential for holistic and transformational adoption of these new indigenous food crop opportunities for African agriculture. Is ‘political will’ the missing sixth capital for sustainable development?

show abstract

New microsatellite markers for Dacryodes edulis (Burseraceae), an indigenous fruit tree species from Central Africa

Cited by 6 publications

References 14 publications

Can seed exchange networks explain the morphological and genetic diversity in perennial crop species? The case of the tropical fruit tree Dacryodes edulis in rural and urban Cameroon

Can seed exchange networks explain the morphological and genetic diversity in perennial crop species? The case of the tropical fruit tree Dacryodes edulis in rural and urban Cameroon

Trees and their seed networks: the social dynamics of urban fruit trees and implications for genetic diversity

The Future of Food: Domestication and Commercialization of Indigenous Food Crops in Africa over the Third Decade (2012–2021)

Contact Info

Product

Resources

About