New microsatellite loci for <i>Narcissus papyraceus</i> (Amarillydaceae) and cross‐amplification in other congeneric species

Simón, Violeta I.; Picó, F. Xavier

doi:10.3732/ajb.1000023

Cited by 11 publications

(12 citation statements)

References 11 publications

(10 reference statements)

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“…The population was not in HWE for any locus supporting a prevalence of assortative or disassortative mating instead of random mating. This result has been observed for other Narcissus species (Simón et al 2010; Simón-Porcar et al 2015b; Barranco et al 2019). More studies involving maternal and paternal origins of seeds in natural pollination conditions will be necessary to clarify which type of mating (assortative or disassortative) is predominant in wild populations of N. broussonetii and the effect in the maintenance of style dimorphism (Simón-Porcar et al 2015b).…”

Section: Discussionsupporting

confidence: 84%

“…The microsatellite loci developed for N. papyraceus and used in several other stylar dimorphic species of this genus (Simón et al 2010; Simón-Porcar et al 2015c; Barranco et al 2019) have been useful estimating the heterozygosity, inbreeding coefficients and other genetic population parameters between morphs in a natural population of N. broussonetii . The estimated genetic diversity parameters for all the plants included in the study (Table 3) were lower than those obtained for N. papyraceus (Simón-Porcar et al 2015c).…”

Section: Discussionmentioning

confidence: 99%

“…We used 10 microsatellite loci to determine the heterozygosity, inbreeding coefficients and other genetic diversity parameters within the population and between morphs to test for possible genetic differences between morphs. These microsatellite loci were developed for N. papyraceus and have been tested in other Narcissus species (Simón et al 2010; Barranco et al 2019). We collected two fresh leaves from each of the plants maintained in the glasshouse (a total of 74 L- and 17 S-plants).…”

Section: Methodsmentioning

confidence: 99%

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Avoiding sexual interference: herkogamy and dichogamy in style dimorphic flowers of Narcissus broussonetii (Amaryllidaceae)

Barranco

Santos‐Gally

2019

AoB PLANTS

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Spatial (herkogamy) or temporal (dichogamy) separation of sex organs are mechanisms considered to restrict self-pollination and promote outcrossing. Additionally, avoidance of self-interference is proposed to be the driving force for the evolution of these mechanisms, particularly in self-incompatible species. However, species with anthers and stigmas at different levels may increase the rate of imprecise pollen transfer, resulting in pollen discounting. Non-reciprocal stylar dimorphism has been considered a transitional, unstable stage towards the evolution of reciprocal style dimorphism (distyly), to simultaneously avoid interference and lack of precision. In this study we investigate the spatial and temporal separation of sex organs in a population of the style dimorphic and self-incompatible Narcissus broussonetii and their consequences in the reciprocity between the sex organs of morphs and their fecundity. First, we evaluated the relative growth of sex organs after anthesis. Then, we studied the stigma receptivity along the flower lifespan including its effect on seed production in both morphs. Finally, given the weak reciprocity between the sex organs of morphs of this species, we estimated population genetic diversity parameters in Long- and Short-styled plants to explore differences between them as a result of rates of inbreeding due to different mating strategies. We observed that Long-styled plants and Short-styled plants present different strategies to avoid sexual interference and both of them had negative consequences in the reciprocity between the sex organs of morphs. Long-styled plants exhibited a delay in stigma receptivity and a higher growth rate of the style after anthesis, while Short-styled plants presented higher herkogamy and no delay in stigma receptivity. These findings suggest that the avoidance of self-interference, in stylar dimorphic Narcissus species, seems to be more critical than improving of reciprocity between the sex organs of morphs. This might explain why reciprocal herkogamy (distyly) is rare in the genus.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Avoiding sexual interference: herkogamy and dichogamy in style dimorphic flowers of Narcissus broussonetii (Amaryllidaceae)

Barranco

Santos‐Gally

2019

AoB PLANTS

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“…DNA was isolated following Bernartzky and Tanksley's () protocol without mercaptoethanol, and each individual was genotyped for four specific microsatellite markers that had high genetic variability and good amplification rates (A116, A121, B104, and B112; Simón et al. ). Polymerase chain reactions (PCRs) were performed in 25 μL of reaction mixture containing 50 ng of template DNA, 1× PCR buffer, 1.5 mM MgCl 2 , 0.1 μM fluorescently labeled (6‐FAM, VIC, NED, and PET dyes) forward primer, 0.1 μM reverse primer, 0.05 mM each dNTP, and 1.25 U Taq polymerase.…”

Section: Methodsmentioning

confidence: 99%

Disassortative mating prevails in style-dimorphicNarcissus papyraceusdespite low reciprocity and compatibility of morphs

Simón‐Porcar

Meagher

2015

Evolution

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Evolution to reduce inbreeding can favor disassortative (inter-morph) over assortative (intramorph) mating in hermaphroditic sexually polymorphic plant species. Heterostyly enhances disassortative pollination through reciprocal placement of stigmas and anthers of morphs and appropriate pollinators. Stylar dimorphism in which there is not reciprocal anther placement may compromise disassortative mating, particularly when there is not intra-morph incompatibility. Variable rates of disassortative mating along with differential female fecundity or siring success among floral morphs could lead to variation in morph ratio. We investigated mating patterns, female fecundity and siring success of style-length morphs in Narcissus papyraceus, a self-incompatible but morph-compatible species with dimorphic (long-and short-styled) and monomorphic (long-styled) populations in central and north regions of its range respectively. We established experimental populations in both regions and exposed them to ambient pollinators. Using paternity analysis, we found similar siring success of morphs and high disassortative mating in most populations. Female fecundity of morphs was similar in all populations. Although these results could not completely explain the loss of dimorphism in the species' northern range, they provided evidence for the evolutionary stability of stylar dimorphism in N. papyraceus in at least some populations. Our findings support the hypothesis that prevailing inter-morph mating is key for the maintenance of stylar dimorphism.

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“…A large number of SSR markers have been developed for Oryza sativa [15], [16]. While some of the SSRs developed for Oryza sativa could be amplified from other AA genomes in the Oryza genus, they were not suitable for cross-amplifications from Oryza species with different genome types [17], as preceding cross-amplifications by Miscanthus sinensis ( Poaceae ) and its relative [18] and Narcissus papyraceus ( Amarillydaceae ) and its relatives [19]. Since there had being no sequence information available for the BBCC genome, no special markers have been developed for it.…”

Section: Introductionmentioning

confidence: 99%

Development of Novel Microsatellite Markers for the BBCC Oryza Genome (Poaceae) Using High-Throughput Sequencing Technology

et al. 2014

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Wild species of Oryza are extremely valuable sources of genetic material that can be used to broaden the genetic background of cultivated rice, and to increase its resistance to abiotic and biotic stresses. Until recently, there was no sequence information for the BBCC Oryza genome; therefore, no special markers had been developed for this genome type. The lack of suitable markers made it difficult to search for valuable genes in the BBCC genome. The aim of this study was to develop microsatellite markers for the BBCC genome. We obtained 13,991 SSR-containing sequences and designed 14,508 primer pairs. The most abundant was hexanuclelotide (31.39%), followed by trinucleotide (27.67%) and dinucleotide (19.04%). 600 markers were selected for validation in 23 accessions of Oryza species with the BBCC genome. A set of 495 markers produced clear amplified fragments of the expected sizes. The average number of alleles per locus (Na) was 2.5, ranging from 1 to 9. The genetic diversity per locus (He) ranged from 0 to 0.844 with a mean of 0.333. The mean polymorphism information content (PIC) was 0.290, and ranged from 0 to 0.825. Of the 495 markers, 12 were only found in the BB genome, 173 were unique to the CC genome, and 198 were also present in the AA genome. These microsatellite markers could be used to evaluate the phylogenetic relationships among different Oryza genomes, and to construct a genetic linkage map for locating and identifying valuable genes in the BBCC genome, and would also for marker-assisted breeding programs that included accessions with the AA genome, especially Oryza sativa.

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New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross‐amplification in other congeneric species

Abstract: These markers will enable genetic diversity studies of N. papyraceus across its distribution range and conduct paternity analyses among individuals differing in flower morphology.

Cited by 11 publications

References 11 publications

Avoiding sexual interference: herkogamy and dichogamy in style dimorphic flowers of Narcissus broussonetii (Amaryllidaceae)

Avoiding sexual interference: herkogamy and dichogamy in style dimorphic flowers of Narcissus broussonetii (Amaryllidaceae)

Disassortative mating prevails in style-dimorphicNarcissus papyraceusdespite low reciprocity and compatibility of morphs

Development of Novel Microsatellite Markers for the BBCC Oryza Genome (Poaceae) Using High-Throughput Sequencing Technology

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