New Methoxyflavone from Casimiroa sapota and the Biological Activities of Its Leaves Extract against Lead Acetate Induced Hepatotoxicity in Rats

Abstract

Flavonoids are agents with strong antioxidant properties and ameliorate many diseases associated with oxidative stress. Leaves of Casimiroa sapota were investigated for components and antioxidant/anti-inflammatory activities against lead acetate ((AcO) Pb) induced hepatotoxicity in rats. Three groups of male albino rats were administrated orally with vehicle or C. sapota (100 and 200 mg/kg b.w/day) for 28 days; other group was injected with sub-acute dose (100 mg/kg b.w/day) of (AcO) Pb. Three protective groups were injected with (AcO) Pb (100 mg/kg b.w/day) for 7 days at day 22 after treatment with either C. sapota (100 or 200 mg/kg b.w/day) or silymarin (SILY) for 28 days. We isolated and identified, from C. sapota, a new compound for the 1st time in nature; 5,6,2',3'-tetramethoxyflavone in addition to the rare compound 5,6,3'-trimethoxyflavone (second report of isolation from nature) and the known compound 5,6,2',3',4'-pentamethoxyflavone. There is an improvement in all hemato-biochemical parameters, antioxidant defense system and anti-inflammatory cytokines of protective groups, which received C. sapota in dose dependent manner. The percentage of changes in all parameters measured in (AcO) Pb groups that received vehicle, CS100, CS200 or SILY were 109.2, 37.3, 12.5%, and 1.2% compared with the healthy control group. The C. sapota groups confer a better antioxidant activity by preventing oxidative stress and inflammation in (AcO) Pb treated rats. The compounds isolated are responsible at least in part for the observed protective effects.

Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts

“…Polyphenols prevent the formation of prostaglandins that cause inflammation [ 38 ]. Tannins and flavonoids also contribute to the anti-inflammatory effect through their ability to inhibit the production of 5 proinflammatory mediators such as lipoxygenase, prostaglandins, cyclooxygenase, serotonin, histamines, and cytokines such as IL-8, TNF- α , or IL-1 β [ 39 – 41 ].…”

Isolation and structure elucidation of compounds from Coccinia grandis leaves extract.

“…However, the significant increase in synthetic drugs' resistance created a demand for exploration for novel classes of drugs with potent biological activity. Low cost, availability and familial experience of herbs made them, again, the best source of new bioactive compounds [2,3]. Nowadays, about 80% of people in the world relay on herbal medicine, which makes it essential to assess their safety and efficacy [1].…”

Investigation of SARS-CoV-2 Main Protease Potential Inhibitory Activities of Some Natural Antiviral Compounds Via Molecular Docking and Dynamics Approaches

“…Nature has provided us with a variety of compounds [36,37], that showed potential antiviral and antioxidant activities [7,38]. The current study indicates compounds having prominent antiviral activity, thus they were selected due to these reported activities.…”