New methods for the determination of aspartame

Prasad, U. Viplava; Divakar, T.E.; Sastry, C. S. P.; Rao, M. V. L.; Kapur, O. P.

doi:10.1016/0308-8146(88)90102-1

Cited by 9 publications

(4 citation statements)

References 12 publications

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“…Sample was scrapped from the TLC plate, eluted from the silica gel, and Fourier transform infrared spectroscopy (FTIR) analysis was performed. The propyl gallate was quantified spectrophotomerically at 560 nm following the method of Prasad et al [21].…”

Section: Synthesis Of Propyl Gallatementioning

confidence: 99%

Propyl Gallate Synthesis Using Acidophilic Tannase and Simultaneous Production of Tannase and Gallic Acid by Marine Aspergillus awamori BTMFW032

Beena

Basheer

Bhat

et al. 2011

Appl Biochem Biotechnol

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Marine Aspergillus awamori BTMFW032, recently reported by us, produce acidophilic tannase as extracellular enzyme. Here, we report the application of this enzyme for synthesis of propyl gallate by direct transesterification of tannic acid and in tea cream solubilisation besides the simultaneous production of gallic acid along with tannase under submerged fermentation by this fungus. This acidophilic tannase enabled synthesis of propyl gallate by direct transesterification of tannic acid using propanol as organic reaction media under low water conditions. The identity of the product was confirmed with thin layer chromatography and Fourier transform infrared spectroscopy. It was noted that 699 U/ml of enzyme could give 60% solubilisation of tea cream within 1 h. Enzyme production medium was optimized adopting Box-Behnken design for simultaneous synthesis of tannase and gallic acid. Process variables including tannic acid, sodium chloride, ferrous sulphate, dipotassium hydrogen phosphate, incubation period and agitation were recognized as the critical factors that influenced tannase and gallic acid production. The model obtained predicted 4,824.61 U/ml of tannase and 136.206 μg/ml gallic acid after 48 h of incubation, whereas optimized medium supported 5,085 U/ml tannase and 372.6 μg/ml of gallic acid production after 36 and 84 h of incubation, respectively, with a 15-fold increase in both enzyme and gallic acid production. Results indicated scope for utilization of this acidophilic tannase for transesterification of tannic acid into propyl gallate, tea cream solubilisation and simultaneous production of gallic acid along with tannase.

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Section: Synthesis Of Propyl Gallatementioning

confidence: 99%

Propyl Gallate Synthesis Using Acidophilic Tannase and Simultaneous Production of Tannase and Gallic Acid by Marine Aspergillus awamori BTMFW032

Beena

Basheer

Bhat

et al. 2011

Appl Biochem Biotechnol

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“…Another common way to study accuracy is with the spike recovery method which adds a known particular concentration of the spiked substance to the sample matrix and measures recovery of its response 17 . Results are listed below in tables (4,5).…”

Section: Accuracymentioning

confidence: 99%

“…Similarly, Aspartame (Fig. 1B) (N-Laspartyl-L-phenylalanine methyl ester), is an artificial sweetener roughly 150-200 times sweeter than table sugar, 5 .…”

Section: Introductionmentioning

confidence: 99%

A Validated HPLC Method for Separation and Determination Aspartame and Acesulfame-K in Food Products

Barakat

Al-Azem²,

Sarkis³

et al. 2022

Bulletin of Pharmaceutical Sciences. Assiut

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A pragmatic, economical, precise, straightforward to execute, and selective method for separation and determination of Aspartame and Acesulfame-k concentrations in non-alcoholic beverages and soft drinks.The separation by HPLC was implemented using a Welchrom C18 column (4.6 x 250 mm, 5µm) at 30°C temperature and a SHIMADZU UV-photo diode array detector at 217, 226 nm for Aspartame and Acesulfame-k respectively.The mobile phase composition consisted of potassium dihydrogen phosphate pH= 4.5 and acetonitrile (80:20 v/v) with a flow rate of 1mL/min and an injection volume of 10 µL.The retention time of Acesulfamek and Aspartame were 2.94, 6.51 respectively. The analysis time was less than 10 minutes. This method demonstrated appropriate results of linearity, precision, and recovery. It was applied efficiently to analyze Aspartame and Acesulfame-k.The calibration curve was linear with R 2 >0.999. The precision values of percentage relative standard deviation were less than 2 %RSD <2.The mean recovery of analytes has ranged between 98.9-101.5, so the method is accurate. The studied analytes were robust at the temperature and pH, whereas Acesulfame-k was robust at the wavelength.

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“…as ninhydrin 5 or N-bromosuccinimide-metol-sulfanilamide. 6 Several ion-selective electrodes have been developed for aspartame: for example, a PVC membrane electrode based on the ion-pair of cetylpyridinium and 2,4,6-trinitrobenzene sulfonate for pharmaceutical formulations. 7 Monitoring the slow reaction of aspartame with 2,4-dinitro-1-fluorobenzene by means of a fluoride-selective electrode makes its kinetic determination possible.…”

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confidence: 99%

Flow-through Spectrophotometric Sensor for the Determination of Aspartame in Low-Calorie and Dietary Products

2004

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A very simple flow-through sensor is presented for the determination of the intense sweetener aspartame in low-calorie and dietary products. The sensor is implemented in a monochannel flow-injection system with UV spectrophotometric detection using a Sephadex CM-C25 cationic exchanger packed 20 mm high in a flow cell. This method is based on the transient retention of a cationic species of the sweetener on the solid phase when a pH 5.0 acetic acid sodium acetate buffer (0.01 M) is used as a carrier (2.6 mL(-1) min). The carrier itself elutes the analyte from the solid support, regenerating a sensing zone. Aspartame was determined by measuring its intrinsic absorbance at 219 nm at its residence time without any derivatization. Calibration graphs were linear over the range of 5.0 - 600.0 microg mL(-1) with an RSD of 0.55% (peak height). This sweetener was determined in several samples by measuring the height or peak area, obtaining recoveries ranging between 95 - 101% and 97.5 - 101%, respectively. The procedure was validated for its use in the determination of aspartame in low-calorie and dietary products, giving reproducible and accurate results.

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New methods for the determination of aspartame

Cited by 9 publications

References 12 publications

Propyl Gallate Synthesis Using Acidophilic Tannase and Simultaneous Production of Tannase and Gallic Acid by Marine Aspergillus awamori BTMFW032

Propyl Gallate Synthesis Using Acidophilic Tannase and Simultaneous Production of Tannase and Gallic Acid by Marine Aspergillus awamori BTMFW032

A Validated HPLC Method for Separation and Determination Aspartame and Acesulfame-K in Food Products

Flow-through Spectrophotometric Sensor for the Determination of Aspartame in Low-Calorie and Dietary Products

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