New methods for high‐accuracy insect chitin measurement

Hahn, Thomas; Roth, Aileen; Febel, Eva; Fijalkowska, Malgorzata; Schmitt, Éric; Arsiwalla, Tarique; Zibek, Susanne

doi:10.1002/jsfa.9044

Cited by 61 publications

(65 citation statements)

References 18 publications

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“…In the present study, cadmium and arsenic were not found, and lead (3.6 mg/kg DM) was below the maximum limit of 10 mg/kg (88% DM) permitted by the EU [33]. The content of chitin in BSFL can be estimated by detergent fibre results (ADF−ADL = 6.60 g/100 g DM; [34]). Chitin has been associated with prebiotic and antimicrobial properties, although it may also be considered as an antinutritional factor when high content is included in the diet [5].…”

Section: Discussioncontrasting

confidence: 50%

Impact of Thermal and High-Pressure Treatments on the Microbiological Quality and In Vitro Digestibility of Black Soldier Fly (Hermetia illucens) Larvae

Campbell

Ortuño

Stratakos

et al. 2020

Animals

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Black soldier fly larvae (BSFL) are gaining importance in animal feeding due to their ability to upcycle low-value agroindustry by-products into high-protein biomass. The present study evaluated the nutritional composition of BSFL reared on brewer’s by-product (BBP) and the impact of thermal (90 °C for 10/15 min) and high-pressure processing (HPP; 400/600MPa for 1.5/10 min) treatments on the microbial levels and in vitro digestibility in both ruminant and monogastric models. BBP-reared BSFL contained a high level of protein, amino acids, lauric acid, and calcium, and high counts of total viable counts (TVC; 7.97), Enterobacteriaceae (7.65), lactic acid bacteria (LAB; 6.50), and yeasts and moulds (YM; 5.07). Thermal processing was more effective (p < 0.05) than any of the HPP treatments in reducing TVC. Both temperature of 90 °C and pressure of 600 MPa reduced the levels of Enterobacteriaceae, LAB, and YM below the detection limit. In contrast, the application of the 400 MPa showed a reduced inactivation (p < 0.05) potential. Heat-treated samples did not result in any significant changes (p > 0.05) on any of the in vitro digestibility models, whereas HPP showed increased and decreased ruminal and monogastric digestibility, respectively. HPP did not seem to be a suitable, cost-effective method as an alternative to heat-processing for the large-scale treatment of BSFL.

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Section: Discussioncontrasting

confidence: 50%

Impact of Thermal and High-Pressure Treatments on the Microbiological Quality and In Vitro Digestibility of Black Soldier Fly (Hermetia illucens) Larvae

Campbell

Ortuño

Stratakos

et al. 2020

Animals

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“…However, considering the final chitin content of diets when substituting 50% of SBM with Hermetia meal, no major impact on pcD of CP can be expected. Nevertheless, there is also a lack of reliable information about the chitin content of insects according to invalid analytical procedures insufficiently taking the N fraction of chitin into account [64].…”

Section: Discussionmentioning

confidence: 99%

Effects of the Partial Replacement of Soybean Meal by Insect or Algae Meal in Chicken Diets with Graded Amino Acid Supply on Parameters of Gut Microbiology and Dietary Protein Quality

Velten¹,

Neumann²,

Schafer³

et al. 2018

OJAS

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“…In contrast, the chitin content of whole insects is generally lower. 78,85 Due to the moderate content, insect breeding only for chitin isolation is not economically feasible without cascade usage of other compounds derived from the insect breeding.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

“…75 H. illucens could be grown on a wide range of organic side streams and contributes to a circular economy. 76,77 Exoskeletons from H. illucens larvae contain up to 35% chitin, 78 which means that it is one of the main compounds that could be isolated from the byproducts of the insect farming industry. 73 Thus, chitin-rich byproducts from insect farming present a new and sustainable source of commercial chitin.…”

Section: Introductionmentioning

confidence: 99%

“…82 To extract chitin from the arthropod cuticle, the purification process remove proteins, lipids, minerals, pigments and catechols contained therein. 83 Whole insects generally contain 30-60% protein, 84 10-25% lipid, 84 5-25% chitin, 50,78,85 5-10% catechols 85 and 2-10% minerals such as calcium, phosphorus, potassium and magnesium salts. 84,86 To date, little has been reported on the extraction methods for insect chitin and its physicochemical properties.…”

Section: Introductionmentioning

confidence: 99%

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Current state of chitin purification and chitosan production from insects

Hahn

Tafi

Paul

et al. 2020

J of Chemical Tech & Biotech

Self Cite

209

113

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Chitin, and especially its deacetylated variant chitosan, has many applications, e.g. as carrier material for pharmaceutical drugs or as a flocculant in wastewater treatment. Despite its versatility and accessibility, chitin, the second most abundant polysaccharide on Earth, has so far been commercially extracted only from crustaceans and to a minor extent from fungi. Insects are a viable alternative source of chitin, but they have not been exploited in the past due to limited availability. Today however, for the sustainable production of animal feed, insect farming is being developed substantially. The availability of large quantities of insect biomass and chitin-rich side products such as exuviae and exoskeletons has been increasing. This review provides an overview of recently published studies of chitin extraction from insects, its subsequent conversion into chitosan and the primary analytical methods used to characterize insect-based chitin and chitosan. We have discovered a large number of research articles published over the past 20 years, confirming the increased attention being received by chitin and chitosan production from insects. Despite numerous publications, we identified several knowledge gaps, such as a lack of data concerning chitin purification degree and chitosan yield. Furthermore, analytical methods used to obtain physicochemical characteristics, structural information and chemical composition meet basic qualitative requirements but do not satisfy the need for a more quantitative evaluation. Despite the current shortcomings that need to be overcome, this review presents encouraging data on the use of insects as an alternative source of chitin and chitosan in the future.

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New methods for high‐accuracy insect chitin measurement

Abstract: According to statistical analysis, ADF-ADL represents a compromise between accuracy and equipment demand and is a suitable method for determining the chitin content of both insects and their residues. © 2018 Society of Chemical Industry.

Cited by 61 publications

References 18 publications

Impact of Thermal and High-Pressure Treatments on the Microbiological Quality and In Vitro Digestibility of Black Soldier Fly (Hermetia illucens) Larvae

Impact of Thermal and High-Pressure Treatments on the Microbiological Quality and In Vitro Digestibility of Black Soldier Fly (Hermetia illucens) Larvae

Effects of the Partial Replacement of Soybean Meal by Insect or Algae Meal in Chicken Diets with Graded Amino Acid Supply on Parameters of Gut Microbiology and Dietary Protein Quality

Current state of chitin purification and chitosan production from insects

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