New method for the determination of endocrine disrupting chemicals in Mediterranean mussel (Mytilus galloprovincialis) using ultra-high performance liquid chromatography–tandem mass spectrometry

García-Fernández, L.; García-Córcoles, María Teresa; Zafra-Gómez, A.; Martín-Pozo, Laura; Hidalgo, F.

doi:10.1016/j.microc.2021.107102

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…Extracts were subjected to d-SPE clean-up by addition of 0.8 g of C18. The type and amount of clean-up sorbent were selected from previous studies reported for the determination of emerging pollutants in mussels based on d-SPE clean-up [ 36 ]. After C18 addition to the tubes, they were hand-shaken for 1 min and centrifuged at 2900 × g for 10 min.…”

Section: Resultsmentioning

confidence: 99%

“…Matrix effect affecting IBU metabolites was reduced just from 10 (for CBX-IBU) to 30% (for 1-OH-IBU) by the use of the internal standard. Therefore, the correction of matrix effect had to be carried out by matrix-matched calibration curves as reported by other authors [ 33 , 35 , 36 ]. Nevertheless, the internal standard was used to compensate residual potential residual matrix effects [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the correction of matrix effect had to be carried out by matrix-matched calibration curves as reported by other authors [ 33 , 35 , 36 ]. Nevertheless, the internal standard was used to compensate residual potential residual matrix effects [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

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Ultrasound-assisted extraction as an easy-to-perform analytical methodology for monitoring ibuprofen and its main metabolites in mussels

et al. 2022

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Non-steroidal anti-inflammatory drugs (NSAIDs) have been reported to be the main pharmaceutical class accumulated in seafood. Among them, ibuprofen (IBU) is of special concern as it is used worldwide to treat common pain, does not require a medical prescription, it is often taken in a high daily dose, and has been reported to cause potential adverse effects on aquatic organisms. IBU is highly transformed into hydroxy- and carboxy-metabolites and/or degradation products generated not only after its administration but also during wastewater treatment or in the environment. These compounds can be present in the environment at higher concentrations than IBU and present higher toxicity. In this work, a low-cost and affordable routine analytical method was developed and validated for the first-time determination of IBU and its main metabolites in mussels. The method is based on ultrasound-assisted extraction (UAE), clean-up by dispersive solid-phase extraction (d-SPE) and analytical determination by liquid chromatography-tandem mass spectrometry. Box-Behnken experimental design was used for method optimisation to better evaluate the influence and interactions of UAE and d-SPE variables. Extraction recoveries were in the range from 81 to 115%. Precision, expressed as relative standard deviation, was lower than 7%. Method detection limits were in the range from 0.1 to 1.9 ng g−1 dry weight. The method was successfully applied to wild mussels. Graphical abstract

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Section: Resultsmentioning

confidence: 99%