New method for standardization of electropherograms obtained in capillary zone electrophoresis

Ikuta, Natsuki; Yamada, Yuji; Yoshiyama, Tatsuya; Hirokawa, Takatsugu

doi:10.1016/s0021-9673(00)00585-9

Cited by 25 publications

(22 citation statements)

References 4 publications

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“…Although different equations have been proposed to calculate the mobilities from experimental data [23], an adequate procedure for CE-MS can be the use of two reference peaks for standardization. The equation originally proposed by Ikuta and colleagues [24] uses one analyte and the EOF marker as reference peaks [24]. However, because no EOF marker is usually observed in CE-MS with a very low pH buffer, two analytes were used in this work as reference.…”

Section: Resultsmentioning

confidence: 99%

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Simplified 2‐D CE‐MS mapping: Analysis of proteolytic digests

Erny

Cifuentes

2007

Electrophoresis

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It has been demonstrated that CE-MS is a very useful hyphenated technique for proteomic studies. However, the huge amount of data stored in a single CE-MS run makes it necessary to account with procedures able to extract all the relevant information made available by CE-MS. In this work, we present a new and easy approach capable of generating a simplified 2-D map from CE-MS raw data. This new approach provides the automatic detection and characterization of the most abundant ions from the CE-MS data including their mass-to-charge (m/z) values, ion intensities and analysis times. It is demonstrated that visualization of CE-MS data in this simplified 2-D format allows: (i) an easy and simultaneous visual inspection of large datasets, (ii) an immediate perception of relevant differences in closely related samples, (iii) a rapid monitoring of data quality levels in different samples, and (iv) a fast discrimination between comigrating polypeptides and ESI-MS fragmentation ions. The strategy proposed in this work does not rely on an excellent mass accuracy for peak detection and filtering, since MS values obtained from an IT analyzer are used. Moreover, the methodology developed works directly with the CE-MS raw data, without interference by the user, giving simultaneously a simplified 2-D map and a much easier and more complete data evaluation. Besides, this procedure can easily be implemented in any CE-MS laboratory. The usefulness of this approach is validated by studying the very similar trypsin digests from bovine, rabbit and horse cytochrome c. It is demonstrated that this simplified 2-D approach allows specific markers for each species to be obtained in a fast and simple way.

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Section: Resultsmentioning

confidence: 99%

“…The electrophoretic mobility values provided by Eq. (3) are logically independent of the applied voltage and capillary dimensions [24]. This point needs to be highlighted, since in CE-MS a large part of the capillary is usually not thermostated.…”

Section: Resultsmentioning

confidence: 99%

Simplified 2‐D CE‐MS mapping: Analysis of proteolytic digests

Erny

Cifuentes

2007

Electrophoresis

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“…Therefore, CE could not be accepted as a routine laboratory technique in this area. Due to these reasons, many workers [36,[66][67][68][69][70][71] have suggested different modifications and alternations to make CE a method of choice for the analysis and speciation of metal ions in different matrices. To achieve good sensitivity and reproducibility, the selection of the capillary wall chemistry, pH and ionic strength of BGE, complexing and visualizing agents, detectors and optimization of BGE have also been described [72][73][74][75][76].…”

Section: Discussionmentioning

confidence: 99%

Metal ion speciation and capillary electrophoresis: Application in the new millennium

2005

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Metal ions are essential for human beings at low concentrations but they are toxic or even carcinogenic at high concentrations. Many metallic ions are found in the environment in different species which are differentiated not only by their physicochemical forms but also by their diverse toxicities with respect to living organisms (speciation). CE has been used for metal ion speciation. The present review article describes the recent trends in metal ion speciation by CE. This article deals with the speciation of metal ions, optimization of the speciation (by independent and dependent variables), hyphenation of CE, validation of the methods, mechanisms of speciation, CE versus chromatography and conclusions.

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“…Another approach for the qualitative and quantitative reproducibilties is the transformation of the total time scale of electrophoretic data into the corresponding effective mobility scale [62,63]. The conversion leads to a better interpretation of the obtained electropherograms in terms of separation, and enables better direct comparison of the electropherograms and easier peak tracking when trying to identify single components from complex matrices, specially when UV-visible signatures of the components are also available [63].…”

Section: Validation Of the Methodsmentioning

confidence: 99%

Chiral resolution of some environmental pollutants by capillary electrophoresis

2003

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The chiral resolution of the environmental pollutants by capillary electrophoresis is reviewed. Various aspects of the chiral resolution such as chiral selectors, optimization of capillary electrophoresis conditions including composition of the background electrolyte (BGE), pH of the BGE, ionic strength of the BGE, structures and types of the chiral selectors, applied voltage, temperature, structures of the chiral pollutants, use of organic modifiers and other parameters are presented. Furthermore, detection, sample treatment, validation of the methods, and the chiral recognition mechanisms, have been discussed.

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New method for standardization of electropherograms obtained in capillary zone electrophoresis

Cited by 25 publications

References 4 publications

Simplified 2‐D CE‐MS mapping: Analysis of proteolytic digests

Simplified 2‐D CE‐MS mapping: Analysis of proteolytic digests

Metal ion speciation and capillary electrophoresis: Application in the new millennium

Chiral resolution of some environmental pollutants by capillary electrophoresis

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