New method for effective identification of adulterated Camellia oil basing on Camellia oleifera-specific DNA

Cheng, Xuexiang; Tao, Yang; Wang, Yunhao; Zhou, Bingqian; Li, Yan; Teng, Linzuo; Wang, Fangbin; Chen, Lili; He, Yan; Guo, Kunpeng; Zhang, Dangquan

doi:10.1016/j.arabjc.2017.12.025

Cited by 48 publications

(22 citation statements)

References 32 publications

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“…When leaves of O. Fragrance were under high temperature stress, ADH gene expression would be increased because of the resistance response mechanism activation in order to avoid from heat damage. Many researches about functional components in the leaves, flowers (Ge et al, 2018a and2018b), barks (Ge et al, 2017a and, stems (Ge et al, 2017c and2017d), roots (Ge et al, 2017e;, fruits (Jiang et al, 2018a and2018b), timbers (Peng et al, 2017a and and byproducts (Liu et al, 2017;Xie et al, 2018) of plants showed that they contain many bioactive components (Peng et al, 2016;He et al, 2017;Cheng et al, 2017;Yang et al, 2017;Qin et al, 2017;Xu et al, 2018;Wang et al, 2018). This result was consistent with the resistance response function of ADH (Strommer et al, 2011).…”

Section: Discussionsupporting

confidence: 69%

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Zhang

2018

Emir J Food Agric

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Because of a lot of aroma components in the flowers, Osmanthus fragrans is widely used as ornamental tree species and its flowers is used as materials of spices and food additives. Alcohol dehydrogenase (ADH) plays an important regulation role in growth, development, stress resistance and aroma synthesis of plants. Using the flowers of O fragrans var. semperflorens as materials, the full-length cDNA of ADH gene (termed OfADH) was successfully cloned through rapid amplification of cDNA ends (RACE), which has the 1128 bp open reading frame (ORF) in length and codes 375 amino acids. The results of conserved domain analysis show that the OfADH belongs to the zinc-dependent medium-chain dehydrogenase /reductase (MDR) superfamily, contains 22 NAD(P) binding sites in the liver_alcohol_DH_like conserved domains, and also has the PLN02740 (Alcohol dehydrogenase-like) domain. The results of semi-quantitative RT-PCR indicate that the expression level of OfADH in O. fragrans flowers is consistent with the rule of aroma formation and release, while the expression of OfADH in leaves is positively correlated with high temperature stress, suggesting that OfADH has obvious functional diversity in regulation of aroma synthesis and response to high temperature stress.

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Section: Discussionsupporting

confidence: 69%

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Zhang

2018

Emir J Food Agric

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“…Studies have shown that the nutritional value of camellia oil is extremely high, with unsaturated fatty acids as high as 80%, among which oleic acid content is as high as 74%-85% [2] . Because of its similar fatty acid composition with olive oil, camellia oil is known as oriental olive oil and is becoming more and more popular among consumers in East Asia [3,4] . Camellia oil has benefits for human health, such as lowering blood pressure and atherosclerosis and scavenging free radicals [5,6] .…”

Section: Introductionmentioning

confidence: 99%

Study on stability and in vitro digestion of camellia oil nanoemulsion system

Zheng

Sun

et al. 2020

E3S Web Conf.

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The soy protein isolate (SPI) was combined with tea saponin as an emulsifier to prepare camellia oil nanoemulsion, and the stability of camellia oil nanoemulsion was compared with that of soybean protein isolate or tea saponin as emulsifier. The effects of different pH, ionic strength, heating temperature and storage time on the average particle size, ξ-potential and microstructure of camellia oil nanoemulsion prepared by three emulsifiers were studied. The results showed that the nanoemulsions prepared by combining natural emulsifiers (SPI-TS) in the pH range of 5-9 were stable and remained stable in the range of 0-300mm NaCl concentration, but had poor tolerance to high salt environment. After heating at different temperatures (30 °C-90 °C) for 30min, the average particle size, ξ-potential and microstructure of the three emulsions did not change significantly, showing good heating stability. At different storage temperatures (4, 25, 50°C) SPI-TS and TS emulsion could exist stably about four weeks, and had good storage stability. In addition, we performed in vitro simulated gastrointestinal digestion studies on the digestive properties of camellia oil nanoemulsions. The results showed that the particle size, the ξ-potential of the nanoemulsion changes depend on the type of emulsifier during digestion. The release rate of free fat acids (FFAs) of nanoemulsions after gastrointestinal digestion were all higher than that of the control group. The results showed that the nanoemulsion delivery system could effectively improve the digestion of camellia oil, It was important to improve the bioavailability of camellia oil.

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“…The use of DNA analysis is more complicated for some vegetable oils (e.g., palm oil) that go through a complex milling and refining process involving high temperatures, which can cause extensive fragmentation of the DNA and compromise its amplification in a PCR assay. Nevertheless, early success has been reported for some of these, namely Camellia oil that is rich in unsaturated fatty acids [104]. In this study, DNA analysis successfully detected adulteration of the Camellia oil, which was not possible via GC analysis of fatty acids [104].…”

Section: Dna Analysis As a Tool For Traceability And Authenticitymentioning

confidence: 77%

“…Nevertheless, early success has been reported for some of these, namely Camellia oil that is rich in unsaturated fatty acids [104]. In this study, DNA analysis successfully detected adulteration of the Camellia oil, which was not possible via GC analysis of fatty acids [104]. In the case of palm oil, Ooi et al [105] found it challenging to extract good quality DNA that was free of impurities from CPO.…”

Section: Dna Analysis As a Tool For Traceability And Authenticitymentioning

confidence: 82%

Sustainable Palm Oil—The Role of Screening and Advanced Analytical Techniques for Geographical Traceability and Authenticity Verification

et al. 2020

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Palm oil production from oil palm (Elaeis guineensis Jacq.) is vital for the economy of Malaysia. As of late, sustainable production of palm oil has been a key focus due to demand by consumer groups, and important progress has been made in establishing standards that promote good agricultural practices that minimize impact on the environment. In line with the industrial goal to build a traceable supply chain, several measures have been implemented to ensure that traceability can be monitored. Although the palm oil supply chain can be highly complex, and achieving full traceability is not an easy task, the industry has to be proactive in developing improved systems that support the existing methods, which rely on recorded information in the supply chain. The Malaysian Palm Oil Board (MPOB) as the custodian of the palm oil industry in Malaysia has taken the initiative to assess and develop technologies that can ensure authenticity and traceability of palm oil in the major supply chains from the point of harvesting all the way to key downstream applications. This review describes the underlying framework related to palm oil geographical traceability using various state-of-the-art analytical techniques, which are also being explored to address adulteration in the global palm oil supply chain.

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New method for effective identification of adulterated Camellia oil basing on Camellia oleifera-specific DNA

Cited by 48 publications

References 32 publications

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Study on stability and in vitro digestion of camellia oil nanoemulsion system

Sustainable Palm Oil—The Role of Screening and Advanced Analytical Techniques for Geographical Traceability and Authenticity Verification

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