New method for detecting slime production by coagulase negative staphylococci.

Abstract

suMMARY An alternative method for detecting the production of slime by coagulase negative staphylococci was compared with the routinely used Christensen method on 124 isolates ofcoagulase negative staphylococci from carriage sites, blood cultures, and infected peritoneal dialysis fluids. The alternative method requires the use ofa specially prepared solid medium-brain heart infusion broth, supplemented with 5% sucrose, and Congo red stain. Of the 124 tests, there was complete agreement between methods in 107 and only one strain was clearly negative by Christensen's method while positive on Congo red agar.The Congo red method is rapid, sensitive, and reproducible and has the advantage that colonies remain viable on the medium. It is also not subject to interbatch variation ofmedia which sometimes affects the reproducibility of the Christensen method.

Evaluation of different detection methods of biofilm formation in the clinical isolates

“…16 There are various methods for biofilm detection. [7][8][9][10][11][12] In this study we evaluated 110 isolates by three screening methods for their ability to form biofilms.…”

“…Freeman et al 9 have described a simple qualitative method to detect biofilm production by using Congo Red Agar (CRA) medium. CRA medium was prepared with brain heart infusion broth (Oxoid, UK) 37 g/L, sucrose 50 g/L, agar No.…”

“…6 There are various methods to detect biofilm production. These include the Tissue Culture Plate (TCP), 7 Tube method (TM), 8 Congo Red Agar method (CRA), 9 bioluminescent assay, 10 piezoelectric sensors, 11 and fluorescent microscopic examination. 12 We screened 110 organisms by three different methods, which could be used in a routine clinical laboratory, for determining their ability to form biofilm.…”

Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

“…Para estudar a capacidade em produzir biofilme todos os isolados foram semeados em Ágar Vermelho Congo (ACR) e posteriormente incubados a 37 o C por até 48h. Após o isolamento, as colônias foram avaliadas quanto à coloração, sendo consideradas positivas aquelas que apresentaram coloração negra (Freeman et al 1989). Como controle positivo da reação foi utilizado uma cepa de Staphylococcus aureus da Americam Type Culture Collection (ATCC 25923).…”

In vitro anti-biofilm and anti-bacterial activity of Junceella juncea for its biomedical application

“…The ability of biofilm formation was studied by the method previously adopted by Freeman et al [8] . Briefly, brain heart infusion (BHI, 37 g/L), sucrose (50 g/L) and agar No.…”