New Method for Catalase Determination

Abstract

6 0.33% gum guaiac, ' 0.08% hydrogen peroxide. d Peroxidase in distilled water.• Peroxidase in pH 6.5 phosphate buffer.and their statistical significance are presented in Table I for unbuffered peroxidase solutions and for peroxidase dilutions prepared in 0.1 M phosphate buffer at pH 6.5; corresponding values for gum guaiac covering the range from 1.0 X 10-4 to 1.0 X 10~6 weight % peroxidase are also given (Table I).Regression lines were calculated using the least mean square method (3).

Non-heme manganese catalase – The ‘other’ catalase

“…It was, of course, the heme-containing catalase enzyme of animal tissues that was responsible for these effects, the enzyme name reflecting its important role in the discovery of biological catalysis. The effervescence resulting from hydrogen peroxide decomposition has been widely used as a qualitative test for catalase activity [9], more recently leading to the discovery of alternative, non-heme catalases in lactic acid bacteria. Since many of these bacteria lack heme and are devoid of cytochromes, the observation of even weak catalase activity was notable, and has been shown to be associated with a class of catalase that is independent of a heme requirement.…”

Molecular Techniques for Understanding the Microbial Community Structure in Mycorrhizosphere

“…Busse et al 1996) Many biochemical, nutritional and physiological characterization tests have been established in bacterial systematics, e.g. tests for indole (Kovacs 1928) oxidation-fermentation (Hugh and Leifson 1953), cytochrome-oxidase (Kovacs 1956;Gaby and Hadley 1957;Cowan and Steel 1964), catalase (Gagnon and Hunting 1959), aminopeptidase (Cerny 1976;Manafi and Kneifel 1990), and KOH-test (Gregersen 1978;Halebian et al 1981;Bourgault and Lamothe 1988). Usually, these tests are carried out in solid and/or liquid media.…”

Evaluation of whole blood catalase estimation for diagnosis of malignancy

“…anticoagulant or venous blood anticoagulated with ethylenediaminetetraacetic acid using the disc flotation method of Gagnon et a1 [9].…”

“…Most tests used for measurement of catalase activity have been time consuming and cumbersome, requiring rigid temperature and pressure controls. The following study was therefore begun to see if measurement of whole blood catalase activity using a simple test system [9] was indeed a practical and useful diagnostic and monitoring test for cancer.…”