New metalloendopeptidase of Morganella morganii ZM

Замалютдинова, Н. М.; Миннуллина, Л. Ф.; Шарипова, М. Р.; Марданова, А. М.

doi:10.1134/s1068162014060156

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Morganella and possibly other Enterobacteriaceae in BSFL guts likely contribute to the digestion of the protein-rich substrate and the breakdown of uric acid, as Morganella morganii possesses genes for proteases (Chen et al, 2012;Zamaliutdinova et al, 2014) and exhibits urease activity (Janda and Abbott, 2015). Furthermore, Morganella, and other Enterobacteriaceae are known for oxidative deamination of various amino acids and nitrate reduction (Janda and Abbott, 2015).…”

Section: The Bsfl Bacterial and Archaeal Communitiesmentioning

confidence: 99%

Cottonseed Press Cake as a Potential Diet for Industrially Farmed Black Soldier Fly Larvae Triggers Adaptations of Their Bacterial and Fungal Gut Microbiota

et al. 2021

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Black soldier fly larvae (Hermetia illucens, Diptera: Stratiomyidae) are used for the bioconversion of organic side products into valuable compounds such as proteins, lipids and chitin. However, the economic competitiveness of farmed insects compared to conventional protein production systems in agriculture and aquaculture depends on the availability of large quantities of inexpensive insect feed. Cottonseed press cake (CPC) is a side-stream of cotton production that is rich in proteins and lipids but unsuitable as feed for several farmed animals, except ruminants, due to the presence of the anti-nutritional sesquiterpenoid gossypol. Here, we tested CPC as a feed for black soldier fly larvae and studied the impact of this diet on the gut microbiome. Larvae reared on CPC developed normally and even showed a shorter life-cycle, but were smaller at the end of larval development than control larvae reared on chicken feed. The adaptability of the larvae to different diets is mediated by their versatile gut microbiome, which facilitates digestion and detoxification. We therefore used amplicon sequencing to analyze the bacterial and fungal communities associated with larvae reared on each diet, revealing differences between the larval guts and frass (residual feed substrate) as well as differences between the two diet groups. For example, Actinomycetaceae and Aspergillaceae were significantly enriched in guts of the CPC diet group and may help to metabolize compounds such as gossypol. Potentially probiotic yeasts and beneficial Enterobacteriaceae, which presumably belong to the core microbiota, were detected in high relative abundance in the gut and frass, indicating a functional role of these microbes, especially the protection against pathogens. We conclude that CPC may be suitable as an inexpensive and environmentally sustainable feed for the industrial rearing of black soldier flies.

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Section: The Bsfl Bacterial and Archaeal Communitiesmentioning

confidence: 99%

Cottonseed Press Cake as a Potential Diet for Industrially Farmed Black Soldier Fly Larvae Triggers Adaptations of Their Bacterial and Fungal Gut Microbiota

et al. 2021

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“…Currently, the activity of PLPs is assayed using protein substrates, most commonly, casein (typically azocasein) 8–16 or less commonly actin 3,15,17 . The applicability of these substrates is somewhat limited.…”

Section: Introductionmentioning

confidence: 99%

An Internally Quenched Fluorescent Peptide Substrate for Protealysin

Karaseva

Chukhontseva

Lemeskina

et al. 2019

Sci Rep

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Protealysin, a metalloprotease of Serratia proteamaculans, is the prototype of a subgroup of the M4 peptidase family. Protealysin-like proteases (PLPs) are widely spread in bacteria but also occur in fungi and certain archaea. The interest in PLPs is primarily due to their putative involvement in the bacterial pathogenesis in animals and plants. Studying PLPs requires an efficient quantitative assay for their activity; however, no such assay has been reported so far. Here, we used the autoprocessing site sequence of the protealysin precursor to construct an internally quenched fluorescent peptide substrate 2-aminobenzoyl-L-arginyl-L-seryl-L-valyl-L-isoleucyl-L-(ε-2,4-dinitrophenyl)lysine. Protealysin and thermolysin, the prototype of the M4 family, proved to hydrolyze only the Ser-Val bond of the substrate. The substrate exhibited a KM = 35 ± 4 μM and kcat = 21 ± 1 s−1 for protealysin as well as a KM = 33 ± 8 μM and kcat = 7 ± 1 s−1 for thermolysin at 37 °C. Comparison of the effect of different enzymes (thermolysin, trypsin, chymotrypsin, savinase, and pronase E) on the substrate has demonstrated that it is not strictly specific for protealysin; however, this enzyme has higher molar activity even compared to the closely related thermolysin. Thus, the proposed substrate can be advantageous for quantitative studies of protealysin as well as for activity assays of other M4 peptidases.

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New metalloendopeptidase of Morganella morganii ZM

Cited by 2 publications

References 16 publications

Cottonseed Press Cake as a Potential Diet for Industrially Farmed Black Soldier Fly Larvae Triggers Adaptations of Their Bacterial and Fungal Gut Microbiota

Cottonseed Press Cake as a Potential Diet for Industrially Farmed Black Soldier Fly Larvae Triggers Adaptations of Their Bacterial and Fungal Gut Microbiota

An Internally Quenched Fluorescent Peptide Substrate for Protealysin

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