From the subterranean waters of the Velika Bukovička špilja Cave near Bukovica village (Tomislavgrad region, Bosnia and Herzegovina) is described and figured one new species of the family Niphargidae (Amphipoda, Gammaridea), Niphargus bukovicensis, sp. n. This species is characterized by presence of elevated number of spines on dactylus of pereopods 6 and 7 only, by numerous setae along dactylus outer margin of gnathopods 1-2, pointed epimeral plate 3 and by elongated inner and outer ramus of uropod 3 in males. The taxonomical relation of this species regarding other similar members of genus Niphargus Schiödte, 1849, is discussed.